Search results for "GREEN"
showing 10 items of 1660 documents
The polypyrimidine tract-binding protein (PTB) is involved in the post-transcriptional regulation of human inducible nitric oxide synthase expression.
2006
Human inducible nitric oxide synthase (iNOS) expression is regulated by transcriptional and post-transcriptional mechanisms. We have recently shown that the multifunctional RNA-binding proteins KH-type splicing regulatory protein and tristetraprolin are critically involved in the post-transcriptional regulation of human iNOS expression. Several reports have shown that KH-type splicing regulatory protein colocalizes with the polypyrimidine tract-binding protein (PTB), and both RNA-binding proteins seem to interact with the same mRNAs. Therefore we analyzed the involvement of PTB in human iNOS expression. In human DLD-1 cells, cytokine incubation necessary to induce iNOS expression did not ch…
Expression and trafficking of fluorescent viral membrane proteins in baculovirus-transduced BHK cells
2004
Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envelope proteins, E1 and E2, of rubella virus (RV) were chosen as a model. The enhanced green fluorescent protein (EGFP) and a red fluorescent protein (RFP) were fused to the C-terminus of E1 and E2, respectively. The proteins were cloned under a cytomegalovirus (CMV) promoter and expressed as fluorescent fusion proteins in baculovirus-transduced baby hamster kidney (BHK) cells. Ex…
Specific Binding of Baculoviruses Displaying gp64 Fusion Proteins to Mammalian Cells
2001
Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG binding domains (ZZ) derived from protein A of Staphylococcus aureus. In addition, the vectors were engineered to incorporate a reporter gene encoding the enhanced green fluorescent protein (EGFP) under the transcriptional regulation of the cytomegalovirus (CMV) IE promoter. Display of the targeting moieties on the viral surface was achieved through fusion to the N-terminus of gp…
Development of a biosensor for copper detection in aqueous solutions using an Anemonia sulcata recombinant GFP.
2014
Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the spectroscopic behaviours of the native and recombinant GFPs were investigated as a function of Cu2+, Cd2+, Pb 2+ and Ni2+ concentration. Our results suggest the high selectivity of both proteins at copper than the other metals and, for the recombinant protein, a great sensitivity at a very low concentration (0.1-1 μM). Moreover, starting from these data, using the combination of …
Growth of zircon and titanite during metamorphism in the granitoid-gneiss terrane south of the Barberton greenstone belt, South Africa
2005
AbstractSHRIMP U-Pb zircon and titanite dating have been used to constrain the timing of mid- to lower- crustal metamorphism (∼650—700°C and 8—11 kbar) and syn-kinematic melting in the granitoid gneiss- dominated terrane south of the Barberton greenstone belt, South Africa. This study is concentrated on a clastic metasedimentary unit exposed in one of several greenstone remnants and a late-kinematic trondhjemite intrusive into spatially associated mixed gneisses. Locally, the clastic metasediments show extensive replacement of garnet and plagioclase by epidote and titanite. The titanites yield an upper intercept date of 3229±9 Ma, and provide a minimum age for the peak of metamorphism. Zirc…
Chemical variations in clay minerals of the Archaean Barberton Greenstone Belt (South Africa)
1996
Abstract Four rock profiles of shales, carbonates and greywacke-type sediments were sampled from the mafic compositional influenced sedimentary Fig Tree Group and from overlying, more felsic related, sedimentary Moodies Group of the Archaean Barberton Greenstone Belt in South Africa. Clay separates of forty sedimentary rocks were examined by XRD and SEM to study the mineralogical, chemical and morphological variations of the constitutive illite and chlorite. The clay minerals showed a significant K-enrichment in the illites and Mg- and Fe-depletions in the chlorites upwards the stratigraphic sequence. Three independent parameters influenced these variations: (1) a change in the origin of th…
Impact of the environmental taxes on reduction of emission from transport in Latvia
2021
Transport has a significant contribution to climate change and many post-Soviet republics, including Latvia, struggle to decrease these emissions to reach carbon neutrality by 2050. In this paper, ...
¿Conduce la neurociencia a una naturalización del deontologismo? «Juicios morales deontológicos» en perspectiva neuroética
2017
A tenor de las investigaciones en el campo de la neuroética y más en concreto en la teoría del proceso dual del juicio moral en autores como Joshua Greene, nos planteamos la cuestión de si la neurociencia conduce necesariamente a una «naturalización del deontologismo». De este modo en nuestra comunicación ponemos en cuestión un modo de hacer neurociencia que pretenda reducir el deontologismo a un tipo de respuesta exclusivamente emocional. Frente a este modelo planteamos otra forma de hacer «neuroética» donde se consideren las estructuras psicofísicas de la moralidad sin incurrir en el reduccionismo miope naturalista. Se trata, por lo tanto, de descubrir la neurociencia de la ética, esto es…
Green taxes, quotas and equality : preserving social justice whilst averting climate change
2016
The need for green fiscal reform is urgent in the face of climate change. Some oppose it, however, arguing that such reforms disproportionately burden poorer individuals whose emissions are far smaller than those of wealthier individuals. Defusing these criticisms, this paper argues that this is not an inevitable feature of green fiscal reform. We should adopt a more scientific attitude not only towards climate change but towards testing fiscal proposals to mitigate it, and avoid dividing, with rushed assumptions, responsible voters who care about both equality and climate change.
Carbon efficiency analysis in the provision of drinking water : Estimation of optimal greenhouse gas emissions
2023
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