Search results for "GRI"
showing 10 items of 10209 documents
Transcriptional study after Beauvericin and Enniatin B combined exposure in Jurkat T cells
2019
Simultaneous mycotoxins toxicity is complex and non-predictable based on their individual toxicities. Beauvericin and Enniatins are emerging mycotoxins highly co-occurrent in food and feed, and their cytotoxicity has been reported in several human cell lines. RNA-seq studies of individual exposure in Jurkat cells demonstrated human genome perturbation mainly affecting mitochondrial pathways, however, both mycotoxins showed differences between their toxic responses. This study investigates the transcriptional effects of combined exposure to Beauvericin and Enniatin B (1:1) (0.1, 0.5, 1.5 μM; 24 h) in Jurkat cells by qPCR on 30 selected target genes (10 mitochondrial, 20 nuclear). Gene expres…
Multifaceted effects of oligodendroglial exosomes on neurons: impact on neuronal firing rate, signal transduction and gene regulation.
2014
Exosomes are small membranous vesicles of endocytic origin that are released by almost every cell type. They exert versatile functions in intercellular communication important for many physiological and pathological processes. Recently, exosomes attracted interest with regard to their role in cell–cell communication in the nervous system. We have shown that exosomes released from oligodendrocytes upon stimulation with the neurotransmitter glutamate are internalized by neurons and enhance the neuronal stress tolerance. Here, we demonstrate that oligodendroglial exosomes also promote neuronal survival during oxygen–glucose deprivation, a model of cerebral ischaemia. We show the transfer from…
L-asparaginase inhibits invasive and angiogenic activity and induces autophagy in ovarian cancer
2012
Recent work identified L-asparaginase (L-ASP) as a putative therapeutic target for ovarian cancer. We suggest that L-ASP, a dysregulator of glycosylation, would interrupt the local microenvironment, affecting the ovarian cancer cell-endothelial cell interaction and thus angiogenesis without cytotoxic effects. Ovarian cancer cell lines and human microvascular endothelial cells (HMVEC) were exposed to L-ASP at physiologically attainable concentrations and subjected to analyses of endothelial tube formation, invasion, adhesion and the assessment of sialylated proteins involved in matrix-associated and heterotypic cell adhesion. Marked reduction in HMVEC tube formation in vitro, HMVEC and ovari…
Pirmsskolas vecuma bērnu patstāvības veicināšana celtniecības rotaļās
2018
Darba autore: Inga Branca Darba nosaukums: “Pirmsskolas vecuma bērnu patstāvības veicināšana celtniecības rotaļās” Darba mērķis: Izzināt pirmsskolas vecuma bērnu patstāvības sekmēšanu celtniecības rotaļās Pētījuma mērķis ir izzināt pirmsskolas bērnu patstāvības sekmēšanu celtniecības rotaļās. Pirmajā nodaļā tiks analizētas pedagogu un psihologu teorijas un atziņas par celtniecības rotaļām un to kā sekmēt bērna patstāvību šajās rotaļās. Šajā nodaļā uzzināsim, kas ir patstāvība un kas nepieciešams, lai to attīstu. Tika izvirzīta hipotēze, ka bērnu patstāvības veidošanās celtniecības rotaļā būs veiksmīga, ja bērnam ir brīvi pieejama vieta, materiāli un laiks rotaļai savas ieceres izstrādei. La…
Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements
2019
Background The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements. Material and methods The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to t…
Porous structure and mechanical strength of cement-lime pastes during setting
2015
Abstract The acceleration of a cement paste setting as a result of lime addition may be shown from isothermal calorimetry measurements. We investigated the underlying mechanisms through two techniques that provide information on porous structure (using Nuclear Magnetic Resonance) and mechanical properties (elastic modulus measured by rheometry). The correlation of the two sets of results on a cement paste clearly reveals the successive steps of setting, and particularly highlights the so-called induction period. We show that this induction period disappears in the presence of lime, leading to an acceleration of the setting. We also show that beyond some critical concentration of added lime …
Prediction of Long-Term Chemical Evolution of a Low-pH Cement Designed for Underground Radioactive Waste Repositories
2012
Low-pH cements, also referred as low-alkalinity cements, are binders with a pore solution pH ≤ 11. They can be designed by replacing significant amounts of Portland cement (OPC) (≥40 %) by silica fume, which can be associated in some cases to low-CaO fly ash and/or ground granulated blast furnace slag to decrease the heat output during hydration by dilution of OPC and improve the mechanical strength of the final material. With the prospect of using these materials in a geological repository, it is of main importance to estimate their long-term properties and the influence of external and internal factors (chemical composition of the binder, storage temperature) on their characteristics. For…
Cement selection criteria for full coverage restorations: A comprehensive review of literature
2021
Background Proper cement selection in fixed prosthesis plays a determinative role in providing long-term serviceability, retention, caries prevention, and patient satisfaction. This study, reviews different luting agent characteristics and their application based on different clinical situations and different types of full coverage restorations. Material and methods An electronic search was conducted through PubMed, Medline, and Google scholar using following keywords or combinations: restoration, full coverage, PFM, porcelain fused to metal, all ceramic, zirconia, ceramic, casting, fixed partial denture, cement*, dental cement, cement selection, and retention. The most related articles wer…
Retention of alkali ions by hydrated low-pH cements: Mechanism and Na+/K+ selectivity
2013
Low-pH cements, also referred to as low-alkalinity cements, can be designed by replacing significant amounts of Portland cement by pozzolanic materials. Their pore solution is characterized by a pH near 11, and an alkali concentration much lower than that of Portland cement. This work investigates the retention of sodium and potassium by a hydrated low-pH cement comprising 60% Portland cement and 40% silica fume. It is shown that sorption of potassium is higher than that of sodium and mainly results from counterion charge balancing of the C-S-H negative surface charge. To explain the greater retention of potassium compared to sodium, it is postulated that potassium, unlike sodium, may enter…
USING ROTATING SPRINKLER GUNS IN CENTRE‐PIVOT IRRIGATION SYSTEMS †
2019
The use of centre-pivot irrigation systems has significantly increased in the last few decades, since they are easier and less time-consuming to manage compared to other irrigation systems. Standard high-pressure impact sprinklers, low-pressure spray sprinklers or LEPA (Low Energy Precision Application) systems are usually combined with a centre-pivot irrigation system (CP-SYS). However, when applying waste water or with reclaimed effluents that are extremely dirty, small nozzle sizes that require CP-SYS could get plugged. As an alternative to common centre-pivot irrigation systems, this paper analyses the ‘geometry’ of areas irrigated by rotating sprinkler guns in a centre-pivot irrigation…