Search results for "Gel"
showing 10 items of 3561 documents
BLASCO CARRASCOSA, Juan Ángel. Miquel Navarro. "Catálogo razonado. Esculturas, dibujos,pinturas y fotografías 1964-2000"
2016
Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis
2017
Background Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4–6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins. Methods Kv extracts were separated by 1D-SDS-PAGE and 2D-DIGE and then underwent mass spectrometric analysis for protein identification. Sarcoidosis and con…
Comparison of pulsed-field gel electrophoresis and whole-genome-sequencing-based typing confirms the accuracy of pulsed-field gel electrophoresis for…
2020
Summary Aim To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to clusters defined by techniques based on whole-genome sequencing (WGS) using Pseudomonas aeruginosa as a model. Methods We selected 65 isolates of ST395 P. aeruginosa isolated in seven European hospitals between 1998 and 2012. Isolates were typed by PFGE and sequenced by WGS. A core genome multi-locus sequence typing (cgMLST) analysis based on 3831 genes was performed with a homemade pipeline. Findings PFGE identified eight pulsotypes and cgMLST differentiated nine clusters and nine singletons. Five cgMLST clusters and pulsotypes (31/65 isolates) coincided perfectly. Isolates …
Shifts in size, genetic structure and activity of the soil denitrifier community by nematode grazing
2010
International audience; Bacterial-feeding nematodes represent an important driver of the soil microbial activity and diversity. This study aimed at characterizing the impact of nematode grazing on a model functional bacterial guild involved in N-cycling, the denitrifiers. Bacterial-feeding nematodes (Cephalobus pseudoparvus) were inoculated into soil microcosms whose indigenous nematofauna had previously been removed. The size, genetic structure and activity of the soil denitrifier community were characterized 15 and 45 days after nematodes inoculation using quantitative PCR of the nirK, nirS and nosZ denitrification genes, fingerprinting of the nirK and nirS genes and denitrification enzym…
Balance as Symbol and Object of Art
2014
The symbolic use of the balance is reviewed, covering mythology and religion, icons and arts, its use in astronomy and astrology as well as in literature and music. In Old Egypt many paintings show men’s height balances symbolically used for soul weighing. In Greece the fate balance was known. In the Roman Empire the symbolic use changed to justice fairness, and legitimacy of coins and this holds until modern time. In the Bible and the Koran fair weighing in trade are demanded. In the Book of Revelation the balance is symbol for misery. Michael archangel became guide of the deceased to the last judgement and weighed the poor sinner against his good deeds. In the witch balance the mass of th…
Solar PAR and UVR modify the community composition and photosynthetic activity of sea ice algae
2015
The effects of increased photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) on species diversity, biomass and photosynthetic activity were studied in fast ice algal communities. The experimental set-up consisted of nine 1.44 m(2) squares with three treatments: untreated with natural snow cover (UNT), snow-free (PAR + UVR) and snow-free ice covered with a UV screen (PAR). The total algal biomass, dominated by diatoms and dinoflagellates, increased in all treatments during the experiment. However, the smaller biomass growth in the top 10-cm layer of the PAR + UVR treatment compared with the PAR treatment indicated the negative effect of UVR. Scrippsiella complex (mainly…
Le Istituzioni di Gaio e il Glücksstern di Niebuhr
2012
Sulla base della documentazione manoscritta, anche inedita, e di altre fonti si ripercorrono le vicende che portarono nel 1816 Barthold Georg Niebuhr a scoprire il palinsesto delle Istituzioni di Gaio nella Biblioteca Capitolare di Verona e si discute criticamente la casualità di tale scoperta.
Der ‚Glücksstern‘ Niebuhrs und die Institutionen des Gaius. Deutsch-italienische Wissenschaftspolitik im frühen 19. Jahrhundert, 2. erweiterte Auflage
2014
In diesem Buch ist die Geschichte der Wiederentdeckung des Palimpsests mit den Gaius-Institutionen durch Barthold Georg Niebuhr neu verhandelt. Die jüngst wiederbelebte These von einer „angekündigten Entdeckung“ wird in Frage gestellt. Das verzerrte Bild des Niebuhrschen Fundes in der Sicht dieser These ist kein Zufall. Es stellt sich vielmehr als später Nachklang wissenschaftlicher Querelen jener Zeit dar. Die These der „angekündigten Entdeckung“ erweist sich bei gründlicher Nachprüfung anhand der Quellen als spekulativ, der Fund ist als eigene Leistung Niebuhrs anzuerkennen. In dieser Abhandlung werden auch bislang unbeachtete oder missverstandene handschriftliche Dokumente textgetreu edi…
Le ultime lettere del carteggio di Niebuhr e Mai (febbraio 1828 – gennaio 1829)
2014
Alla luce delle ultime lettere del carteggio fra Barthold Georg Niebuhr e Angelo Mai, di cui si pubblica il testo sulla base degli originali, si ricostruiscono i rapporti fra i due studiosi.
A low repeat length in oligodendrocyte chromatin
1985
Abstract: The behavior of oligodendrocyte chromatin after micrococcal nuclease digestion of nuclei was assayed in brains of rats of four different ages. During oligodendrocyte differentiation, a decreasing sensitivity of the chromatin to enzymatic attack was observed. On the other hand, the nucleosomal repeat length showed a slight tendency to increase during development. It is worth noting that even the highest values reported here for “oligodendrocyte’ chromatin repeat lengths are significantly lower than 200 base pairs, the value previously reported by others for “non‐astrocytic glia.” Copyright © 1985, Wiley Blackwell. All rights reserved