Search results for "Gene expression."
showing 10 items of 4076 documents
Role of dioxygenase α-DOX2 and SA in basal response and in hexanoic acid-induced resistance of tomato (Solanum lycopersicum) plants against Botrytis …
2015
Resistance of tomato (Solanum Lycopersicum) to the fungal pathogen Botrytis cinerea requires complex interplay between hormonal signalling. In this study, we explored the involvement of new oxylipins in the tomato basal and induced response to this necrotroph through the functional analysis of the tomato α-dioxygenase2 (α-DOX2)-deficient mutant divaricata. We also investigated the role of SA in the defence response against this necrotrophic fungus using SA-deficient tomato nahG plants. The plants lacking dioxigenase α-DOX2, which catalyses oxylipins production from fatty acids, were more susceptible to Botrytis, and hexanoic acid-induced resistance (Hx-IR) was impaired; hence α-DOX2 is requ…
Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes▿
2010
ABSTRACT Enzymes involved in oxidation of long-chain n -alkanes are still not well known, especially those in Gram-positive bacteria. This work describes the alkane degradation system of the n -alkane degrader actinobacterium Gordonia sp. strain SoCg, which is able to grow on n -alkanes from dodecane (C 12 ) to hexatriacontane (C 36 ) as the sole C source. SoCg harbors in its chromosome a single alk locus carrying six open reading frames (ORFs), which shows 78 to 79% identity with the alkane hydroxylase (AH)-encoding systems of other alkane-degrading actinobacteria. Quantitative reverse transcription-PCR showed that the genes encoding AlkB (alkane 1-monooxygenase), RubA3 (rubredoxin), RubA4…
Absence of the endo-beta-1,4-glucanases Cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato.
2007
Cel1 and Cel2 are members of the tomato (Solanum lycopersicum Mill) endo-beta-1,4-glucanase (EGase) family that may play a role in fruit ripening and organ abscission. This work demonstrates that Cel1 protein is present in other vegetative tissues and accumulates during leaf development. We recently reported the downregulation of both the Cel1 mRNA and protein upon fungal infection, suggesting the involvement of EGases in plant-pathogen interactions. This hypothesis was confirmed by assessing the resistance to Botrytis cinerea infection of transgenic plants expressing both genes in an antisense orientation (Anti-Cel1, Anti-Cel2 and Anti-Cel1-Cel2). The Anti-Cel1-Cel2 plants showed enhanced …
Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model
2006
Abstract Background The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new play…
Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study
2010
Background: Insect diapause is an important biological process which involves many life-history parameters important for survival and reproductive fitness at both individual and population level. Drosophila montana, a species of D. virilis group, has a profound photoperiodic reproductive diapause that enables the adult flies to survive through the harsh winter conditions of high latitudes and altitudes. We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes…
Sparse relative risk survival modelling
2016
Cancer survival is thought to closed linked to the genimic constitution of the tumour. Discovering such signatures will be useful in the diagnosis of the patient and may be used for treatment decisions and perhaps even the development of new treatments. However, genomic data are typically noisy and high-dimensional, often outstripping the number included in the study. Regularized survival models have been proposed to deal with such scenary. These methods typically induce sparsity by means of a coincidental match of the geometry of the convex likelihood and (near) non-convex regularizer.
Molecular and agronomic responses to soil inoculation with plant-growth promoting rhizobacteria and arbuscular mycorrhizae in Triticum durum
2012
Plant-growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal (AM) fungi contribute to plant nutrient uptake by increasing the availability of nutrients and the root adsorbing surface. However, the molecular mechanisms of plant responses to these associations are still under-investigated. Gene expression analysis could lead to the identification of biomarkers usable to early select genotypes for an increased nutrient uptake efficiency. The main objective of this study was to determine the effects of these associations on plant total biomass and grain yield in durum wheat (cv. Anco Marzio). Secondly, we aimed to analyze the expression pattern of key genes involved in nutrient uptake…
Supplementary data to: Sequence and Regulatory Variation in Acetylcholinesterase Genes Contribute to Insecticide Resistance in Different populations …
2021
This is data used in Aigi Margus, Saija Piiroinen, Philipp Lehmann, et al. Sequence and Regulatory Variation in Acetylcholinesterase Genes Contribute to Insecticide Resistance in Different populations of Leptinotarsa decemlineata. Authorea. September 26, 2021. Data contains bioassay data, enzyme activity data, gene expression data and gene sequence data used to research insect herbivores resistance to insecticides. See the attached data description file for more information.
Original data for article: Acute and chronic response to a change in salinity of the euryhaline polychaete Pygospio elegans (Claparède)
2019
These data were collected in an experimental study designed to evaluate the ability of the polychaete Pygospio elegans to cope with an acute as well as a long-term change in salinity. Specimens from Herslev, Denmark, originating from a salinity of about 15 were exposed to salinity 15 as control and 5 and 30 as low and high salinity treatments. After acute exposure, we measured body volume changes as well as gene expression of seven genes related to volume- and osmoregulation within 4 hours of exposure.
Testing chemical carcinogenicity by using a transcriptomics HepaRG-based model?
2014
The EU FP6 project carcinoGENOMICS explored the combination of toxicogenomics and in vitro cell culture models for identifying organotypical genotoxic- and non-genotoxic carcinogen- specific gene signatures. Here the performance of its gene classifier, derived from exposure of metabolically competent human HepaRG cells to prototypical non-carcinogens (10 compounds) and hepatocarcinogens (20 compounds), is reported. Analysis of the data at the gene and the pathway level by using independent biostatistical approaches showed a distinct separation of genotoxic from non-genotoxic hepatocarcinogens and non-carcinogens (up to 88 % correct prediction). The most characteristic pathway responding to …