Search results for "Generation"

The naturalization of the almond Prunus dulcis in different ecological contexts in the Mediterranean: An underestimated process?

2022

Although Prunus dulcis has been frequently listed in non-native floras all around the world, its naturalization has been rarely studied in the field. Probably, because this species has generally invaded synanthropic habitats. We assessed the density of natural regeneration of almond, biometric parameters (height and basal stem diameter), and the distribution in life stage classes in three different ecological contexts in Sicily (Mediterranean Italy): one natural habitat (maquis), one pine afforestation and two prickly pear orchards. We recorded 236 naturally regenerating almond individuals, with density ranging from 140 individuals per hectare (prickly pear orchard 1) up to 2,400 individual…

Spatio-temporal patterns and drivers of secondary succession in a Mediterranean landscape

2017

In recent years, many Mediterranean agricultural lands have been abandoned, reforested, rewilded and ecologically restored, creating different templates and trajectories for the development of secondary succession. Our objective was to verify whether past legacies of woody vegetation (remnant forest, shrub patches and reforestations) influenced the current spatial pattern of woody cover in a mid-successional Mediterranean area, identifying spatial cold and hotspots (i.e: low/slow or high/fast cover increase) and looking for footprints of seed-disperser and herbivore-mediated processes at landscape and local scales. The study took place in three 500x200 m plots in a formerly managed and part…

Effect of 2,4-D and 4-CPPU on somatic embryogenesis from stigma and style transverse thin cell layers of Citrus

2002

Callus induction, somatic embryogenesis and plant regeneration were obtained in lemon [Citrus limon (L.) Burm. cv `Femminello'] and sweet orange [C. sinensis (L.) Osb. cv `Washington Navel GS'] from cultures of stigma and style transverse thin cell layer explants [(t)TCLs]. Explants were cultured on 16 different media, based on the nutrients and vitamins of Murashige and Tucker medium (MT) supplemented with different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-Nprime-phenylurea (4-CPPU). Sucrose (146 mM) was used as the sole carbon source. Somatic embryos arose from callus at the surface of stigma and style (t)TCLs 3–5 months after culture initiation of…

Evaluation of Alpha-Synuclein Cerebrospinal Fluid Levels in Several Neurological Disorders

2022

(1) Background: Alpha-synuclein (α-syn) is a presynaptic neuronal protein that regulates several neuronal functions. In recent decades, the role of α-syn as a biomarker of neurodegenerative diseases has been explored, especially in synucleinopathies. However, only a few studies have assessed its role as biomarker in other neurological disorders. The aim of the study was to evaluate cerebrospinal fluid (CSF) α-syn levels in several neurological disorders; (2) Methods: We measured CSF α-syn levels by a commercial ELISA kit in 158 patients classified in the following group: controls, Alzheimer’s Disease (AD), cerebrovascular diseases, inflammatory central nervous …

Retinal neurodegeneration and an innovative nanostructured approach in an iron overload in vivo model

2020

New frontiers in regenerative medicine in cardiology: the potential of Wharton's jelly mesenchymal stem cells.

2013

Cardiomyopathies are still the first cause of death in the world. The identification of resident stem cells, comprising those derived from sub-endocardial stroma, suggests the possible self regeneration of the heart under autocrine/paracrine modulation in the cardiac microenvironment. Nevertheless, because of the limited in vivo regeneration potential of damaged cardiac tissue, the use of drugs and ultimately cardiac transplantation remain the common treatments of heart diseases and defects. The differentiative potential of embryonic and mesenchymal stem cells (MSCs) derived from different tissues (such as bone marrow and adipose tissue) was extensively explored in cell therapy for regenera…

Magnetic Resonance Spectroscopy: principles and clinical applications

2009

Evaluation of a Cell-Free Collagen Type I-Based Scaffold for Articular Cartilage Regeneration in an Orthotopic Rat Model.

2020

The management of chondral defects represents a big challenge because of the limited self-healing capacity of cartilage. Many approaches in this field obtained partial satisfactory results. Cartilage tissue engineering, combining innovative scaffolds and stem cells from different sources, emerges as a promising strategy for cartilage regeneration. The aim of this study was to evaluate the capability of a cell-free collagen I-based scaffold to promote cartilaginous repair after orthotopic implantation in vivo. Articular cartilage lesions (ACL) were created at the femoropatellar groove in rat knees and cell free collagen I-based scaffolds (S) were then implanted into right knee defect for the…

Current Perspectives on Adult Mesenchymal Stromal Cell-Derived Extracellular Vesicles: Biological Features and Clinical Indications.

2022

Extracellular vesicles (EVs) constitute one of the main mechanisms by which cells communicate with the surrounding tissue or at distance. Vesicle secretion is featured by most cell types, and adult mesenchymal stromal cells (MSCs) of different tissue origins have shown the ability to produce them. In recent years, several reports disclosed the molecular composition and suggested clinical indications for EVs derived from adult MSCs. The parental cells were already known for their roles in different disease settings in regulating inflammation, immune modulation, or transdifferentiation to promote cell repopulation. Interestingly, most reports also suggested that part of the properties of pare…

Can Early Life Exposure to Permethrin lead to intergenerational effects?

2016

Pesticides are largely used in agriculture against pests and consequently are present in fruits and vegetables. The wide presence of pesticide residues in breast milk underline the risk for the population, focalizing the long-term consequence of early life pyrethroid exposure. The significant presence of pyrethroid metabolites in the urine of population over the world confirms that their presence in food is a global problem. It has been demonstrated that there is a correlation between the environmental exposure to pesticides and the development of neurodegenerative diseases. Neonatal exposure to Permethrin (PERM), a member of the family of synthetic pyrethroids, can induce neurodegeneration…