Search results for "Genetica"
showing 10 items of 906 documents
DNMT1 SILENCING ELICITS DIFFERENT CELL CYCLE RESPONSES IN PRIMARY VERSUS TUMOR CELLS AND IS ASSOCIATED WITH ANEUPLOIDY GENERATION
2011
DNMT1 depletion activates a pathway p14ARF/TP53 controlled that induces G1 arrest preventing DNA demethylation and aneuploidy
2011
Background: Aneuploidy is considered the result of chromosome segregation errors caused by defects in the mitotic spindle assembly, centrosome duplication, cell-cycle checkpoints and epigenetic changes. Usually, aneuploidy affects negatively proliferation of normal cells. However, it is frequently associated with cancer that is characterized by a uncontrolled proliferation. Thus, understanding the pathway(s) that block proliferation of aneuploid cells might open new avenue to exploit new cancer therapies. O bservations: We found that in primary human fi broblasts (IMR90) knocking down of DNMT1, a member of epigenetic machinery is perceived by the cell as a stress signal that induces p14ARF …
CMYC PROMOTER BINDINGPROTEIN 1 (MBP1) AS A NOVEL MARKER TO IDENTIFY NEW SUBTYPES OF ERBB2 NEGATIVE BREAST TUMORS
2011
La Genetica ... sulle tavole di Ustica
2019
Quale variabile crescente fa in modo che dei gemelli monozigoti, che notoriamente condividono DNA identico, mostrino, dalla nascita alla vecchiaia, sempre maggiori differenze fra loro? Si potrebbe intuitivamente rispondere: il tempo e l’ambiente, o meglio il tempo passato a vivere in un diverso ambiente. Ma fino a qualche decennio fa nessun genetista avrebbe mai ammesso che l’interazione fra il DNA e l’ambiente potesse influenzare l’espressione genica anche non mutando il DNA. Oggi, invece, e ormai da circa 15 anni, è oggetto di studio, l’Epigenetica, secondo la quale possono esistere modificazioni chimiche ed ereditabili del DNA che non scaturiscono da mutazioni della sua sequenza nucleoti…
METHYLOMIC SIGNATURE AND MOLECULAR MODELLING TO BETTER UNDERSTAND AUTOPHAGY INDUCED BY PHYTOCHEMICAL IN CACO-2 CELLS
2019
The binomial “autophagy-cancer” is intricate and methylomic studies can help to understand it by changing point of view from a gene level to an -omic one. Recently, autophagy-modulating properties of several phytochemicals have attracted attention in anticancer research. We evaluated whether Indicaxanthin (IND), the peculiar known beneficial phytochemical of prickly pear, seasonally available in the southern Italy, could induce autophagy in Caco2 cells, and whether it results from an epigenomic modification and/or a direct molecular interaction. IND increased autophagy in Caco-2 cells; the methylomic signature, obtained by Reduced Representation Bisulfite Sequencing (15 million of clusters)…
Farmacogenética de la Tuberculosis: Nuevo modelo de predicción de hepatotoxicidad inducida por fármacos antituberculosis
2017
Introducción: La hepatotoxicidad inducida por fármacos antituberculosis (HIFA) es una reacción adversa grave y potencialmente fatal del tratamiento de la tuberculosis (TB). Tres de los cuatro fármacos utilizados como terapia de primera línea (isoniacida, rifampicina, pirazinamida), han sido asociados a HIFA. Estudios sobre farmacogenética de la TB han asociado el desarrollo de HIFA con variaciones en genes de enzimas que metabolizan estos fármacos. Objetivos: Debido a que en Argentina la TB es una enfermedad re-emergente y a la elevada prevalencia de HIFA encontrada en pacientes internados, nos propusimos evaluar la posible asociación de factores ambientales y variantes genéticas en enzimas…