Search results for "Genome sequencing"

showing 8 items of 188 documents

Differential Contribution of the Parental Genomes to a

2019

In European regions of cold climate, S. uvarum can replace S. cerevisiae in wine fermentations performed at low temperatures. S. uvarum is a cryotolerant yeast that produces more glycerol, less acetic acid and exhibits a better aroma profile. However, this species exhibits a poor ethanol tolerance compared with S. cerevisiae. In the present study, we obtained by rare mating (non-GMO strategy), and a subsequent sporulation, an interspecific S. cerevisiae × S. uvarum spore-derivative hybrid that improves or maintains a combination of parental traits of interest for the wine industry, such as good fermentation performance, increased ethanol tolerance, and high glycerol and aroma productions. G…

genome sequencingS. uvarumwine fermentationartificial hybridfungifood and beveragesBioengineering and BiotechnologySaccharomyces cerevisiaeRNA-seqethanol toleranceOriginal ResearchFrontiers in bioengineering and biotechnology
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Data from: The population genomics of archaeological transition in west Iberia: investigation of ancient substructure using imputation and haplotype-…

2018

We analyse new genomic data (0.05–2.95x) from 14 ancient individuals from Portugal distributed from the Middle Neolithic (4200–3500 BC) to the Middle Bronze Age (1740–1430 BC) and impute genomewide diploid genotypes in these together with published ancient Eurasians. While discontinuity is evident in the transition to agriculture across the region, sensitive haplotype-based analyses suggest a significant degree of local hunter-gatherer contribution to later Iberian Neolithic populations. A more subtle genetic influx is also apparent in the Bronze Age, detectable from analyses including haplotype sharing with both ancient and modern genomes, D-statistics and Y-chromosome lineages. However, t…

medicine and health carehaplotypesLife SciencesMedicineGenome sequencingPaleogenetics
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Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain

2020

This article belongs to the Special Issue Bacteriophage—Molecular Studies.

phage therapyPhage therapyPhage therapyKlebsiella pneumoniae<i>Klebsiella pneumoniae</i>medicine.medical_treatmentGenome ViralArticleHost SpecificityCatalysisMicrobiologylcsh:ChemistryInorganic ChemistryBacteriophageViral Proteins03 medical and health sciencesPodoviridaeProtein DomainsbacteriophagemedicineHumansBacteriophagesTypingPhysical and Theoretical ChemistryBacteriophagelcsh:QH301-705.5Molecular BiologyPhylogenySpectroscopy030304 developmental biologyWhole genome sequencingInfectivityLikelihood Functions0303 health sciencesbiology030306 microbiologyOrganic ChemistryGeneral Medicinebiology.organism_classification3. Good healthComputer Science ApplicationsKlebsiella pneumoniaelcsh:Biology (General)lcsh:QD1-999Lytic cycleSpainInternational Journal of Molecular Sciences
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Genome degeneration and adaptation in a nascent stage of symbiosis

2014

Symbiotic associations between animals and microbes are ubiquitous in nature, with an estimated 15% of all insect species harboring intracellular bacterial symbionts. Most bacterial symbionts share many genomic features including small genomes, nucleotide composition bias, high coding density, and a paucity of mobile DNA, consistent with long-term host association. In this study, we focus on the early stages of genome degeneration in a recently derived insect-bacterial mutualistic intracellular association. We present the complete genome sequence and annotation of Sitophilus oryzae primary endosymbiont (SOPE). We also present the finished genome sequence and annotation of strain HS, a close…

pseudogènePseudogene[SDV]Life Sciences [q-bio]Molecular Sequence DataIS elements;comparative genomics;degenerative genome evolution;pseudogenes;recent symbiontpseudogenesBacterial genome sizedegenerative genome evolutioncomparative genomicsBiologyGenomeIS elementsEvolution Molecular03 medical and health sciencesEnterobacteriaceaeGeneticsAnimalsdonnée de séquence moléculaireInsertion sequenceSymbiosisGeneEcology Evolution Behavior and SystematicsComputingMilieux_MISCELLANEOUS030304 developmental biology2. Zero hungerGeneticsComparative genomicsWhole genome sequencing0303 health sciencesBase Sequence030306 microbiologygénomique comparativeAdaptation PhysiologicalColeopterarecent symbiontAdaptationsymbiosedégradation du génomeGenome Bacterialséquence d'insertionResearch Article
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Data from: Genomics of speciation and introgression in Princess cichlid fishes from Lake Tanganyika

2016

How variation in the genome translates into biological diversity and new species originate has endured as the mystery of mysteries in evolutionary biology. African cichlid fishes are prime model systems to address speciation-related questions for their remarkable taxonomic and phenotypic diversity, and the possible role of gene flow in this process. Here, we capitalize on genome sequencing and phylogenomic analyses to address the relative impacts of incomplete lineage sorting, introgression and hybrid speciation in the Neolamprologus savoryi-complex (the ‘Princess cichlids’) from Lake Tanganyika. We present a time-calibrated species tree based on whole-genome sequences and provide strong ev…

recombination biasmedicine and health carewhole genome sequencingNeolamprologusMedicinegenomic landscapesLinked selectionhuman activitiesLife sciencesintrogressive hybridization
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Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MF(T.).

2014

The neotype strain Methanobacterium formicicum MFT (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074 bp in size, featuring a GC content of 41.23%. M. formicicum MFT encodes several genes predicted to be involved in adaptation to abiotic stress such as high osmolarity. The strain MFT is of biotechnological importance since M. formicicum strains are often found in production-scale biogas plants and it is suggested as a starter culture for the anaerobic biomethanation process. (C) 2014 Elsevier B.V. All rights reserved.

synthesisMethanogenesisMolecular Sequence DataBioengineeringMethanogenesisApplied Microbiology and BiotechnologyGenomeWaste Disposal FluidMicrobiologyGeneWhole genome sequencingStrain (chemistry)biologyInoculant cultureBase SequenceMethanobacteriumCompatible soluteGeneral MedicineSequence Analysis DNAbiology.organism_classificationBiogas productionMethanogenMethaneGC-contentSludgeGenome BacterialBiotechnologyJournal of biotechnology
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Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity▿ †

2008

ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.

virusesMutantMolecular Sequence DataMutation MissenseGenetics and Molecular BiologyBacillus PhagesGenome ViralViral Plaque AssayApplied Microbiology and BiotechnologySyntenyBacteriophageBacteriolysisGene OrderPoint MutationBacillus (shape)Whole genome sequencingGeneticsEcologybiologyBase SequenceTectivirusVirionSequence Analysis DNAbiology.organism_classificationBacillus anthracisOpen reading frameBacillus anthracisDNA ViralTectiviridaeFood ScienceBiotechnologyTectiviridae
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A New Phylogenetic Framework for the Animal-Adapted

2018

Tuberculosis (TB) affects humans and other animals and is caused by bacteria from the Mycobacterium tuberculosis complex (MTBC). Previous studies have shown that there are at least nine members of the MTBC infecting animals other than humans; these have also been referred to as ecotypes. However, the ecology and the evolution of these animal-adapted MTBC ecotypes are poorly understood. Here we screened 12,886 publicly available MTBC genomes and newly sequenced 17 animal-adapted MTBC strains, gathering a total of 529 genomes of animal-adapted MTBC strains. Phylogenomic and comparative analyses confirm that the animal-adapted MTBC members are paraphyletic with some members more closely relate…

whole-genome sequencingspecificityhost rangegenetic diversityMicrobiologyOriginal Researchhost–pathogen interactionsFrontiers in microbiology
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