Search results for "Gram"
showing 10 items of 9069 documents
Artesunate Activates Mitochondrial Apoptosis in Breast Cancer Cells via Iron-catalyzed Lysosomal Reactive Oxygen Species Production
2011
The antimalarial agent artesunate (ART) activates programmed cell death (PCD) in cancer cells in a manner dependent on the presence of iron and the generation of reactive oxygen species. In malaria parasites, ART cytotoxicity originates from interactions with heme-derived iron within the food vacuole. The analogous digestive compartment of mammalian cells, the lysosome, similarly contains high levels of redox-active iron and in response to specific stimuli can initiate mitochondrial apoptosis. We thus investigated the role of lysosomes in ART-induced PCD and determined that in MCF-7 breast cancer cells ART activates lysosome-dependent mitochondrial outer membrane permeabilization. ART impac…
p62: Friend or Foe? Evidences for OncoJanus and NeuroJanus Roles
2020
p62 is a versatile protein involved in the delicate balance between cell death and survival, which is fundamental for cell fate decision in the context of both cancer and neurodegenerative diseases. As an autophagy adaptor, p62 recognizes polyubiquitin chains and interacts with LC3, thereby targeting the selected cargo to the autophagosome with consequent autophagic degradation. Beside this function, p62 behaves as an interactive hub in multiple signalling including those mediated by Nrf2, NF-κB, caspase-8, and mTORC1. The protein is thus crucial for the control of oxidative stress, inflammation and cell survival, apoptosis, and metabolic reprogramming, respectively. As a multifunctional pr…
Autophagy in development and stress responses of plants.
2006
The uptake and degradation of cytoplasmic material by vacuolar autophagy in plants has been studied extensively by electron microscopy and shown to be involved in developmental processes such as vacuole formation, deposition of seed storage proteins and senescence, and in the response of plants to nutrient starvation and to pathogens. The isolation of genes required for autophagy in yeast has allowed the identification of many of the corresponding Arabidopsis genes based on sequence similarity. Knockout mutations in some of these Arabidopsis genes have revealed physiological roles for autophagy in nutrient recycling during nitrogen deficiency and in senescence. Recently, markers for monitor…
Involvement of PAR-4 in Cannabinoid-Dependent Sensitization of Osteosarcoma Cells to TRAIL-Induced Apoptosis
2014
The synthetic cannabinoid WIN 55,212-2 is a potent cannabinoid receptor agonist with anticancer potential. Experiments were performed to determine the effects of WIN on proliferation, cell cycle distribution, and programmed cell death in human osteosarcoma MG63 and Saos-2 cells. Results show that WIN induced G2/M cell cycle arrest, which was associated with the induction of the main markers of ER stress (GRP78, CHOP and TRB3). In treated cells we also observed the conversion of the cytosolic form of the autophagosome marker LC3-I into LC3-II (the lipidated form located on the autophagosome membrane) and the enhanced incorporation of monodansylcadaverine and acridine orange, two markers of t…
Muzikālo darbu izmantošanas un reproducēšanas tiesiskie aspekti.
2022
Jebkurai ar mūzikas industriju saistītai personai ir svarīgi apzināties, kāda ir viņa loma autortiesībās. Svarīgi saprast kādas tiesības tiek aizsargātas un kādas ir jāievēro, lai nepārkāptu citas personas tiesības. Ar muzikālajiem darbiem saistītās autortiesības un blakustiesības ir ļoti fragmentētas, jo viena muzikāla darba radīšanā tiek iesaistīti dažādi subjekti un veidojas daudz dažādu tiesību. Darba mērķis ir noskaidrot, kā var uzlabot pašreizējo Latvijas autortiesību regulējumu, lai tas būtu piemērots veidiem, kādos mūsdienās tiek radīti un izmantoti muzikālie darbi, tā lai netiktu ierobežotas citas personu tiesības un brīvības. Darba izstrādes procesā autors nonāca pie vairākiem sec…
Autortiesību uz datorprogrammu īstenošanas īpatnības.
2022
Informācijas un komunikāciju tehnoloģiju nozare mūsdienās ir viena no Latvijas straujāk augošajām un pelnošākajām tautsaimniecības nozarēm. Šī nozare pēdējo gadu laikā ir apliecinājusi lielu eksporta potenciālu, 2019. gadā eksportam sasniedzot vienu miljardu eiro. Informācijas tehnoloģiju izstrāde var izpausties divos veidos: 1) aparatūras (angļu valodā – “hardware”) izstrādē; 2) programmatūras (angļu valodā – “software”) izstrādē. Lai nodrošinātu nozares drošu attīstību un veicinātu sadarbību nozares speciālistu un komersantu starpā, nepieciešams iespēju robežās jau laicīgi novērst potenciālas juridiskās neskaidrības. Tajā skaitā arvien lielāku uzmanību nepieciešamas pievērst tieši intelek…
Evolution Within a Bizarre Phylum: Homologies of the First Echinoderms
1998
SYNOPSIS. The Extraxial/Axial Theory (EAT) of echinoderm skeletal homologies describes two major body wall types: axial and extraxial. The latter is subdivided into perforate and imperforate regions. Each of the regions has a distinctly different source in early larval development. Axial skeleton originates in the rudiment, and develops in association with the pentaradially arranged hydrocoel according to specific ontogenetic principles. Perforate and imperforate extraxial regions are associated with the left and right somatocoels respectively, are not governed by ontogenetic principles of plate addition, and are products of the non-rudiment part of the larval body. The morphology of even t…
Enterococcal meningitis caused by Enterococcus casseliflavus. First case report
2005
Abstract Background Enterococcal meningitis is an uncommon disease usually caused by Enterococcus faecalis and Enterococcus faecium and is associated with a high mortality rate. Enterococcus casseliflavus has been implicated in a wide variety of infections in humans, but never in meningitis. Case presentation A 77-year-old Italian female presented for evaluation of fever, stupor, diarrhea and vomiting of 3 days duration. There was no history of head injury nor of previous surgical procedures. She had been suffering from rheumatoid arthritis for 30 years, for which she was being treated with steroids and methotrexate. On admission, she was febrile, alert but not oriented to time and place. H…
2020
BAG3, a multifunctional HSP70 co-chaperone and anti-apoptotic protein that interacts with the ATPase domain of HSP70 through its C-terminal BAG domain plays a key physiological role in cellular proteostasis. The HSP70/BAG3 complex determines the levels of a large number of selective client proteins by regulating their turnover via the two major protein degradation pathways, i.e. proteasomal degradation and macroautophagy. On the one hand, BAG3 competes with BAG1 for binding to HSP70, thereby preventing the proteasomal degradation of its client proteins. By functionally interacting with HSP70 and LC3, BAG3 also delivers polyubiquitinated proteins to the autophagy pathway. BAG3 exerts a numbe…
Pharmacy brands "Mēness Aptieka" and "BENU aptieka"communication in social media Instagram and Facebook
2020
Bakalaura darba “Farmācijas zīmolu “Mēness Aptieka” un “BENU aptieka” komunikācija sociālajos medijos Instagram un Facebook” mērķis ir noskaidrot, kas ietekmē aptiekas zīmola izvēli, kā arī –“BENU aptieka” un “Mēness Aptieka” komunikācijas tendences un to izmaiņas vīrusa "Covid-19" laikā “Instagram” un “Facebook" platformās. Darba teorētiskā bāze balstās uz akadēmisko literatūru par zīmolu, satura mārketingu, sociālajiem medijiem un auditorijas uztveri. Metodoloģijā tiek aprakstītas bakalaura darbā izmantotās pētījuma metodes, kas ir daļēji strukturēta intervija, internetaptauja un kvantitatīvā kontentanalīze. Empīriskajā daļā tiek aprakstīti iegūtie rezultāti.