Search results for "Green"
showing 10 items of 1660 documents
Destabilized green fluorescent protein detects rapid removal of transcription blocks after genotoxic exposure
2007
High stabilities of reporter proteins and their messenger RNAs (mRNAs) interfere with the detection of rapid transient changes in gene expression, such as transcriptional blocks posed by genotoxic DNA lesions. We have modified a green fluorescent protein (GFP) gene within the episomal pMARS vector by addition of a fragment encoding for mouse ornithine decarboxylase (ODC) proline-glutamate-serine-threonine-rich (PEST) sequence in order to target the protein to the proteasomes and achieved an unprecedentedly fast GFP turnover in permanently transfected human cells. As early as 1 h after inhibition of protein synthesis by cycloheximide, the number of fluorescent cells decreased more than 5-fo…
Towards light-mediated sensing of bacterial comfort
2013
Abstract Bacterial comfort is central to biotechnological applications. Here, we report the characterization of different sensoring systems, the first step within a broader synthetic biology-inspired light-mediated strategy to determine Escherichia coli perception of environmental factors critical to bacterial performance. We did so by directly ‘asking’ bacterial cultures with light-encoded questions corresponding to the excitation wavelength of fluorescent proteins placed under the control of environment-sensitive promoters. We built four genetic constructions with fluorescent proteins responding to glucose, temperature, oxygen and nitrogen; and a fifth construction allowing UV-induced exp…
Chloroplast morphology and pyrenoid ultrastructural analyses reappraise the diversity of the lichen phycobiont genus Trebouxia (Chlorophyta)
2022
Abstract Trebouxiophyceae is a wide class of green algae comprising coccoid and elliptic unicells, filaments, blades and colony-forming species that occur in diverse terrestrial and aquatic environments. Within this class, the genus Trebouxia Puymaly is among the most widespread lichen phycobionts worldwide. However, the 29 formally described species based on the combination of morphological traits and genetic diversity woefully underrepresented the overall species-level diversity recognized in the genus. In Trebouxia, reliable differentiation and characterization of the species-level lineages can be achieved by studying the diversity of key diagnostic features of pyrenoid ultrastructure an…
Intraguild interactions between two egg parasitoids exploring host patches
2010
Intraguild interactions between two egg parasitoids, Trissolcus basalis (Hymenoptera: Scelionidae) and Ooencyrtus telenomicida (Hymenoptera: Encyrtidae), exploring egg masses of the Southern Green Stink Bug (SGSB) Nezara viridula (Heteroptera: Pentatomidae), were investigated in laboratory conditions by single, simultaneous and sequential host attack experiments. Mortality of N. viridula eggs was higher in simultaneous and sequential releases compared to single species releases. In simultaneous host exploitations, T. basalis females displayed an aggressive behavior against O. telenomicida females. The outcome of multiparasitism showed that interspecific larval competition was dominated by O…
The response of Trissolcus basalis to footprint contact kairomones from Nezara viridula females is mediated by leaf epicuticular waxes.
2009
Chemical footprints left behind by true bugs are perceived as contact kairomones by scelionid egg parasitoids. Female wasps encountering a contaminated artificial substrate display a characteristic arrestment posture, holding the body motionless and antennating the surface. In the system Nezara viridula (L.) and its egg parasitoid Trissolcus basalis (Wollaston), previous studies have shown that the kairomone mediating such behavior is part of N. viridula's cuticular hydrocarbons (CHC) and furthermore that the wasp's ability to discriminate host male and female footprints is mainly based on the presence/absence of nonadecane (nC(19)). In this study, the effect of epicuticular waxes of leaves…
The Tropical Forest Ecosystem: Reviewing the Effects of Deforestation on Climate and Environment
1991
The rapid decline of tropical rain forests in the 1980s, particularly caused by man-made forest clearance for farmland and timber exploitation, is accompanied by adverse climatic and environmental effects. In particular, rainfall and temperature conditions are adversely changed, with lower rainfall and high-er temperatures. Through deforestation, the atmospheric emissions of CO2 are increased and thus the global greenhouse effect is being strengthened. Tropical deforestation also increases soil erosion, depending upon the type of ecosystem. It also can be seen that floods more often occur in cases of deforestation. An international “action programme” for the protection and preservation of t…
Global sensitivity of aviation NO<sub>x</sub> effects to the HNO<sub>3</sub>-forming channel …
2013
Abstract. The impact of a recently proposed HNO3-forming channel of the HO2 + NO reaction on atmospheric ozone, methane and their precursors is assessed with the aim to investigate its effects on aviation NOx induced radiative forcing. The first part of the study addresses the differences in stratospheric and tropospheric HOx-NOx chemistry in general, by comparing a global climate simulation without the above reaction to two simulations with different rate coefficient parameterizations for HO2 + NO → HNO3. A possible enhancement of the reaction by humidity, as found by a laboratory study, particularly reduces the oxidation capacity of the atmosphere, increasing methane lifetime significantl…
The Constraints of Vehicle Range and Congestion for the Use of Electric Vehicles for Urban Freight in France
2015
The 9th International Conference on City Logistics, Tenerife, Espagne, 17-/06/2015 - 19/06/2015; Electric vehicle is a solution to reduce pollutant emissions from road urban freight. This paper assesses the potential CO2 reduction by transferring urban freight from diesel to electric vehicles while simultaneously looking at the two main technical constraints: electric vehicle range and the impact on congestion linked to change diesel heavy duty vehicles (with a load up to 25 tons) to much smaller electric vehicles. The data used has been computed from a survey (ECHO) that describes in details a very large sample of French shipments. Two scenarios were set up, which differ mainly by the type…
Research on the Origin of Strawberry Plug Plants in Soilless Cultivation
2008
In recent years, the main advances in strawberry cultivation in Italy have regarded cultivation techniques, the use of alternatives to methyl bromide and the type of propagation material used. Fresh plants have taken over from cold-stored plants in 60% of cases. This prompted the need for new studies on the cultivation of fresh strawberry plants carried out in Sicilian nurseries. This report illustrates the results of a trial carried out in Sicily to find the best plug plants grown by four nurseries located at different altitudes (Madonie mountains, Sicani mountains, Nebrodi mountans) and on the plain (Marsala) and with an open cycle soilless system. The trial was carried out at our experim…
Induction of rapid and reversible cytokeratin filament network remodeling by inhibition of tyrosine phosphatases
2002
The cytokeratin filament network is intrinsically dynamic, continuously exchanging subunits over its entire surface, while conferring structural stability on epithelial cells. However, it is not known how cytokeratin filaments are remodeled in situations where the network is temporarily and spatially restricted. Using the tyrosine phosphatase inhibitor orthovanadate we observed rapid and reversible restructuring in living cells, which may provide the basis for such dynamics. By examining cells stably expressing fluorescent cytokeratin chimeras, we found that cytokeratin filaments were broken down and then formed into granular aggregates within a few minutes of orthovanadate addition. After …