Search results for "Green"
showing 10 items of 1660 documents
C57BL/6-specific conditions for efficient in utero electroporation of the central nervous system.
2014
Abstract Background In utero electroporation is a fast an efficient tool to specifically address gene expression in the murine central nervous system. This technique was originally established in ICR/CD-1 outbred mice. Neuroanatomical differences between the different mouse strains and variations in gestation length require the optimization of the conditions for each strain to avoid severe complications. Furthermore the relevant position information is currently only scarcely standardized and not always easy to transfer to C57BL/6 mice. New method In this study we present an improved method for in utero electroporation of C57BL/6 including a detailed atlas that allows for specific and effic…
Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model.
2009
Summary Background The angiogenic potential of vascular endothelial growth factor (VEGF) and its oxygen pressure-dependent regulation suggest a strong connection between this growth factor and the ‘delay phenomenon'. In this study we focused on the chronological changes in VEGF concentration and flap perfusion in order to optimise the duration of surgical delay. Methods The VEGF concentration in skin and underlying muscle was measured in oversized, random-pattern flaps on 38 male Sprague-Dawley rats after 3, 5 or 7 days of surgical delay. Additionally, flaps were raised 5 or 7 days past preconditioning. The effect on flap perfusion was measured using indocyanine green fluoroscopy and the si…
Exclusive transduction of human CD4+ T Cells upon systemic delivery of CD4-targeted lentiviral vectors
2015
Abstract Playing a central role in both innate and adaptive immunity, CD4+ T cells are a key target for genetic modifications in basic research and immunotherapy. In this article, we describe novel lentiviral vectors (CD4-LV) that have been rendered selective for human or simian CD4+ cells by surface engineering. When applied to PBMCs, CD4-LV transduced CD4+ but not CD4− cells. Notably, also unstimulated T cells were stably genetically modified. Upon systemic or intrasplenic administration into mice reconstituted with human PBMCs or hematopoietic stem cells, reporter gene expression was predominantly detected in lymphoid organs. Evaluation of GFP expression in organ-derived cells and blood …
Sustainable Development and Technological Impact on CO2 Reducing Conditions in Romania
2015
Climate change is a reality all over the world, and its complexity is increasing. Therefore, sustainability has become a national and international concern, ingrained in many organizational processes. The ability of organizations to respond to sustainability concerns is sometimes hindered by the complexity of integrating sustainability into business models and by the need to rethink their strategic directions. In Romania, sustainable development has become a priority for businesses, but even though companies are showing some concern, there are yet to demonstrate any full commitment (they are mainly concerned with areas such as society and the environment). This paper assesses Romania’s invo…
Pollutant Emissions in Ports: A Comprehensive Review
2021
In recent decades, maritime transport demand has increased along with world population and global trades. This is associated with higher pollution levels, including the emissions of GHG and other polluting gases. Ports are important elements within maritime transport and contribute themselves to pollutant emissions. This paper aims to offer a comprehensive yet technical review of the latest related technologies, explaining and covering aspects that link ports with emissions, i.e., analyzing, monitoring, assessing, and mitigating emissions in ports. This has been achieved through a robust scientific analysis of very recent and significant research studies, to offer an up-to-date and reliable…
A comparative study on campus sustainability in higher education sector in Hong Kong and Finland
2015
This thesis presents a comparative study examining the practices of campus sustainability in Higher Education Sectors between Hong Kong and Finland. Campus sustainability is a trend during the last decade as a result of numerous declarations and meetings for sustainable development in Higher education all over the world. In this thesis, the investigated areas are focused on environmental sustainability and Social sustainability. This study used the content analysis method to analyze public available documents from the selected fifteen Higher education institutions in Hong Kong and Finland. The first stage was a description of the current overall situations of campus sustainability in the tw…
Intrahippocampal transplantation of mesenchymal stromal cells promotes neuroplasticity
2012
Multipotent mesenchymal stromal cells (MSC) secrete soluble factors that stimulate the surrounding microenvironment. Such paracrine effects might underlie the potential benefits of many stem cell therapies. We tested the hypothesis that MSC are able to enhance intrinsic cellular plasticity in the adult rat hippocampus.Rat bone marrow-derived MSC were labeled with very small superparamagnetic iron oxide particles (VSOP), which allowed for non-invasive graft localization by magnetic resonance imaging (MRI). Moreover, MSC were transduced with lentiviral vectors to express the green fluorescent protein (GFP). The effects of bilateral MSC transplantation on hippocampal cellular plasticity were a…
A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing
2002
We describe here the definition and characterization of antigen CT-8/HOM-TES-85 encoded by a previously unknown gene and identified by serological expression screening using antibodies from a seminoma patient. Intriguingly, the leucine zipper region of CT-8/HOM-TES-85 shows an atypical amphipathy with clusters of hydrophobic residues that is exclusively shared by the N-myc proto-oncogene. CT-8/HOM-TES-85 gene is tightly silenced in normal tissues except for testis. However, it is frequently activated in human neoplasms of different types including lung cancer, ovarian cancer, melanoma and glioma. Endogenous as well as heterogeneously expressed CT-8/HOM-TES-85 targets predominantly to the nu…
Abstract 3015: Precise investigation of cancer stem cells in mouse glioblastoma
2018
Abstract In this study, we employ mouse models to investigate features and roles of cancer stem cells (CSCs) in glioblastoma (GBM). A nestin-TK-GFP transgene is firstly used to label CSCs in a fully penetrant mouse model of GBM (M7: hGFAP-Cre; Nf1fl/+; p53fl/fl; Ptenfl/+). Food-mediated ganciclovir (GCV) delivery kills proliferative transgene positive cells and significantly prolongs the lives of the transgene bearing mice. Isolation and transplantation of the tumor cells indicates the GFP+ cells are more tumorigenic than the GFP- cells. We then generate and characterize a novel transgene (CGD: nestin-CreERT2-H2BeGFP-hDTR) that labels all the neural stem/progenitor cells in the subventricul…
Sentinel lymph node mapping with indocyanine green in cervical cancer patients undergoing open radical hysterectomy: a single-institution series
2020
Abstract Purpose To assess the rate of bilateral sentinel lymph node (SLN) detection with indocyanine green (ICG), to evaluate the sensitivity and the negative predictive value of cervical cancer patients undergoing open radical hysterectomy; to compare open versus minimally invasive SLN biopsy performance and to assess factors related to no/unilateral SLN mapping. Methods We retrospectively reviewed consecutive patients with FIGO 2018 stage IA1 with lymph-vascular space involvement to IIB and IIIC1p cervical carcinoma who underwent SLN mapping with ICG followed by systematic pelvic lymphadenectomy between 05/2017 and 06/2020. Patients were divided according to surgical approach for statist…