Search results for "HAp"
showing 10 items of 2767 documents
Chemically induced mouse models of intestinal inflammation
2007
Animal models of intestinal inflammation are indispensable for our understanding of the pathogenesis of Crohn disease and ulcerative colitis, the two major forms of inflammatory bowel disease in humans. Here, we provide protocols for establishing murine 2,4,6-trinitro benzene sulfonic acid (TNBS)-, oxazolone- and both acute and chronic dextran sodium sulfate (DSS) colitis, the most widely used chemically induced models of intestinal inflammation. In the former two models, colitis is induced by intrarectal administration of the covalently reactive reagents TNBS/oxazolone, which are believed to induce a T-cell-mediated response against hapten-modified autologous proteins/luminal antigens. In …
Hsp60, a Novel Target for Antitumor Therapy: Structure-Function Features and Prospective Drugs Design
2013
Heat shock protein 60 kDa (Hsp60) is a chaperone classically believed to be involved in assisting the correct folding of other mitochondrial proteins. Hsp60 also plays a role in cytoprotection against cell stressors, displaying for example, antiapoptotic potential. Despite the plethora of studies devoted to the mechanism of Hsp60's function, especially in prokaryotes, fundamental issues still remain unexplored, including the definition of its role in cancer. Key questions still unanswered pertain to the differences in structure-function features that might exist between the well-studied prokaryotic GroEL and the largely unexplored eukaryotic Hsp60 proteins. In this article we discuss these …
Chaperonopathies and chaperonotherapy. Hsp60 as therapeutic target in cancer: potential benefits and risks.
2013
In this minireview we focus on Hsp60 as a target for anticancer therapy. We discuss the new concepts of chaperonopathies and chaperonotherapy and present information on Hsp60 localization in the cell membrane of human tumor cells. We describe novel mechanisms for Hsp60 reaching the extracellular environment that involve membrane-associated stages, as well as data on anti-Hsp60 antibodies found in human sera, both in normal subjects and patients affected by autoimmune diseases. Finally, we discuss possible therapeutic applications of anti-Hsp60 antibodies in cancer treatment, evaluating also side effects on non-tumor cells. In conclusion, the way for investigating Hsp60-targeted anti-tumor t…
Sea Urchin Mitochondrial Matrix Contains a 56-kDa Chaperonine-like Protein
1997
Abstract Paracentrotus lividus mitochondrial matrix contains a constitutive hsp of 56-KDa which cross reacts with a serum anti-hsp-60 chaperonine from yeast mitochondria. The localization of hsps preexisting or newly synthesized in different subcellular fractions of gastrula embryos is also analyzed by two-dimensional electrophoresis.
Hsp60 in Modifications of Nervous System Homeostasis and Neurodegeneration
2019
Hsp60 is a critical chaperonin for its role in preserving cell survival and protecting mitochondria against stress conditions. Indeed, mutations or malfunctions of Hsp60 are involved in several human diseases, either genetic or acquired, some of them affecting also the brain. In this chapter, we present several experimental observations supporting the role of Hsp60 in some neurodegenerative diseases. Further, Hsp60, as multifunctional protein, contributes to the protein folding system, to protect mitochondria and is involved in several other cellular pathways that are known to be affected in these diseases. Furthermore, due to its role outside of the mitochondria and in the extracellular fl…
The importance of the insular cortex for vestibular and spatial syndromes.
2020
BACKGROUND AND PURPOSE The aim of the study was to identify the neuroanatomical correlates and associations of neuropsychological syndromes after acute unilateral right-hemisphere brain lesions. The neuropsychological syndromes considered were orientation in three-dimensional space such as tilts of the subjective visual vertical or of the subjective haptic vertical, pusher syndrome, visual neglect and unawareness of paresis (anosognosia for hemiparesis). These neuropsychological phenomena have been found to occur separately or in different combinations after lesions to the right insular cortex. METHOD Magnetic resonance imaging scans were obtained from 82 patients with acute right-hemispher…
Oxidative DNA cleavage mediated by a new unexpected [Pd(BAPP)][PdCl4] complex (BAPP = 1,4-bis(3-aminopropyl)piperazine): crystal structure, DNA bindi…
2022
A novel Pd(II) double complex, [Pd(BAPP)][PdCl4], containing the 1,4-bis(3-aminopropyl)piperazine (BAPP) ligand is investigated. X-ray crystallography of a single crystal confirmed the structure of the [Pd(BAPP)][PdCl4] complex. The spectroscopic behavior was also elucidated using elemental analysis, nuclear magnetic resonance and Fourier-transform infrared spectroscopy, and mass spectrometry. The antimicrobial susceptibility of the [Pd(BAPP)][PdCl4] complex against all tested microbial strains was lower than that of the BAPP ligand except for C. albicans. The cytotoxic impacts of the BAPP ligand and its [Pd(BAPP)][PdCl4] complex were evaluated in vitro for HepG2, CaCo-2 and MCF7 cell lines…
Neuromuscular, hormonal and oxidative stress responses to endurance running exercises in well-trained runners
2007
The aim of this study was to investigate to which type of running exercises well trained endurance runners respond more favourably with regard to acute neuromuscular and endocrinological responses and exercise-induced oxidative stress. It was hypothesized that these responses depend on the type of running exercise and relate to the physical characteristics. Altogether nine different running exercises and a long walking exercise were performed by a total of 88 male athletes. An intermittent exercise consisting of progressive 20 s runs decreased vertical jump performance in middle distance runners (MID), but improved it in marathon runners (MAR). An 18 – 24 min exhaustive test run, a 40 min i…
The role of technological culture in the theoretical dichotomy between technique and form/Il ruolo della cultura tecnologica nella dicotomia teorica …
2017
La cultura tecnologica spiega il rapporto tra forma e tecnica basandosi su una dimensione concreta ed esistenziale. «La dimensione esistenziale “verità” si manifesta attraverso la storia, ma il suo significato trascende la situazione storica. Tale concretizzazione dipende in genere dal come siano le cose fatte, ossia dalle forme e dalla tecnologia» (Schulz, 1979). In tal ottica il saggio esplora teorie e prassi della cultura tecnologica sul rapporto tra materia e forma e tra architettura e tecnica, a partire dal concetto di tecnologia: dall’evoluzione storica delle tecniche e dei sistemi costruttivi al valore dell’innovazione, sondando nelle problematiche ideologiche, sociali e materiali ch…
Bar-sur-Aube (Aube). 13 rue Beugnot et 2, 4, 10 rue du Prieuré [notice archéologique]
2019
Un projet immobilier de réhabilitation de l’îlot du prieuré Saint-Pierre (dépendance de l’abbaye Saint-Claude dans le Jura), accolé à l’église éponyme, au NO de la ville ancienne de Bar-sur-Aube, a entraîné la prescription d’un diagnostic archéologique sur les deux « cours » concernées par le projet et sur une analyse du potentiel archéologique conservé en élévation. Ce diagnostic a montré d’importants aménagements médiévaux encore conservés au sein du prieuré Saint-Pierre dont la création est antérieure au milieu du XIIe s., tant dans les élévations que dans les données stratigraphiques. Bien que les remaniements modernes et contemporains soient conséquents, et caractéristiques d’une opéra…