Search results for "HAp"
showing 10 items of 2767 documents
Data from: Extinction and recolonization of maritime Antarctica in the limpet Nacella concinna (Strebel, 1908) during the last glacial cycle: toward …
2013
Quaternary glaciations in Antarctica drastically modified geographical ranges and population sizes of marine benthic invertebrates and thus affected the amount and distribution of intraspecific genetic variation. Here, we present new genetic information in the Antarctic limpet Nacella concinna, a dominant Antarctic benthic species along shallow ice-free rocky ecosystems. We examined the patterns of genetic diversity and structure in this broadcast spawner along maritime Antarctica and from the peri-Antarctic island of South Georgia. Genetic analyses showed that N. concinna represents a single panmictic unit in maritime Antarctic. Low levels of genetic diversity characterized this population…
Data from: Seasonal dynamics of Atlantic herring (Clupea harengus L.) populations spawning in the vicinity of marginal habitats
2015
Gillnet sampling and analyses of otolith shape, vertebral count and growth indicated the presence of three putative Atlantic herring (Clupea harengus L.) populations mixing together over the spawning season February–June inside and outside an inland brackish water lake (Landvikvannet) in southern Norway. Peak spawning of oceanic Norwegian spring spawners and coastal Skagerrak spring spawners occurred in March–April with small proportions of spawners entering the lake. In comparison, spawning of Landvik herring peaked in May–June with high proportions found inside the lake, which could be explained by local adaptations to the environmental conditions and seasonal changes of this marginal hab…
Data from: The population genomics of archaeological transition in west Iberia: investigation of ancient substructure using imputation and haplotype-…
2018
We analyse new genomic data (0.05–2.95x) from 14 ancient individuals from Portugal distributed from the Middle Neolithic (4200–3500 BC) to the Middle Bronze Age (1740–1430 BC) and impute genomewide diploid genotypes in these together with published ancient Eurasians. While discontinuity is evident in the transition to agriculture across the region, sensitive haplotype-based analyses suggest a significant degree of local hunter-gatherer contribution to later Iberian Neolithic populations. A more subtle genetic influx is also apparent in the Bronze Age, detectable from analyses including haplotype sharing with both ancient and modern genomes, D-statistics and Y-chromosome lineages. However, t…
Evidence for Shape Coexistence in Neutron-Rich Rh and Ag Nuclei
1988
Neutron-rich nuclei of Rh and Ag have been investigated by γ-ray spectroscopic measurements as well as single-particle transfer reactions like (d,3He) and (3He,d). The obtained level schemes of 105,107,109,111Rh and 113,115Ag show two different types of excitation: (I) proton-hole states related to a spherical shape of the nucleus and (II) proton-particle states with a rotational band-like structure (intruder band) pointing towards deformation of the nucleus. The “fingerprints” for such a shape coexistence and resulting systematics in these nuclei and the possible interpretation of the intruder band as a rotational band built upon the 1/2+[431] Nilsson configuration are discussed.
Hsp60 in Skeletal Muscle: From Molecular Anatomy to Pathophysiology
2019
The chaperoning system of an organism is composed of the entire set of chaperones, co-chaperones, and chaperone co-factors and their interactors and receptors. Its functions pertain typically to protein homeostasis but also to many other activities inside and outside cells. In the skeletal muscle, with its multi-molecular structures rich in proteins and their continuous rearrangements, the chaperoning system plays a crucial role. However, little is known about the details of the workings of the chaperoning system in skeletal muscle development and during exercise and disease. Molecular chaperones are surely involved in muscle formation and maintenance under physiologic conditions and under …
Optical tomography from focus
2007
A model and a method providing a 3D reconstruction of a given translucent object from a series of image acquisitions performed with various focus tunings is proposed. The object is imaged by transmission; refraction, reflection and diffusion effects are neglected. It is modeled as a stack of translucent parallel slices and the acquisition process can be described by a set of linear equations. We propose an efficient inversion technique with O(n) complexity, allowing practical applications with a simple laptop computer in a very reasonable time. Examples of results obtained with a simulated 3D translucent object are presented and discussed.
Hapten Design and Antibody Generation for Immunoanalysis of Spirotetramat and Spirotetramat-enol
2018
Spirotetramat - a tetramic acid insecticide - is rapidly metabolized or degraded to give spirotetramat-enol; so, common residue definitions include the sum of both compounds. In the present study, two spirotetramat-functionalized derivatives (haptens) have been designed to generate immunoreagents to these molecules for rapid immunochemical analysis. Haptens have been synthesized with alternative linker tethering sites and, for the first time, high-affinity antibodies have been generated with different specificities to these active principles. Two sensitive assays have been developed using the same antibody in different formats, and by using linker-site heterologous haptens, the selectivity …
Rationally designed haptens for highly sensitive monoclonal antibody-based immunoanalysis of fenhexamid.
2018
Immunochemical methods have been consolidated during the last few years as complementary analytical strategies for chemical contaminant and residue determination. However, generation of suitable immunoreagents for small organic molecules demands adequate hapten design. In this study, fenhexamid was considered as a model compound and novel haptens were designed and synthesized in order to evaluate the influence of the linker tethering site on antibody binding properties and immunoassay parameters. Haptens were conceived with the spacer arm at different positions, while the more antigenic aromatic moiety was kept free. The synthesis of these functionalized compounds was accomplished by total …
Generation of anti-azoxystrobin monoclonal antibodies from regioisomeric haptens functionalized at selected sites and development of indirect competi…
2012
Azoxystrobin is a modern strobilurin fungicide used around the world to combat prime diseases affecting highly valuable crops. Accordingly, residues of this chemical are frequently found in food, even though mostly under maximum tolerated levels. We herein describe the development of an indirect competitive immunoassay for the determination of azoxystrobin residues. A panel of monoclonal antibodies displaying subnanomolar affinity to azoxystrobin was generated using, as immunizing haptens in mice, four functionalized derivatives carrying the same spacer arm located at different rationally chosen positions. This collection of antibodies was thoroughly characterized with homologous and hetero…
General considerations in the interpretation of I-J genetic restrictions: evidence that the antigen-binding chain of antigen-specific T-suppressor fa…
1987
SUMMARY (CBA × B10)F1 [(H-2k x H-2b)] mice produce two types of antigen-specific T-suppressor factor (TsF), which can be separated by affinity chromatography on anti-I-J monoclonal antibody. After reduction and alkylation, both chains of F1 TsF are required for biological activity. However, the antigen-binding chain (AgBC) of F1 TsFk (AgBCk) is only complemented by I-Jk and likewise for F1 TsFb. In other words, interchain complementation shows the same genetic restriction in interchain complementation in parental and F1 mice. F1 TsF bearing, for example, I-Jk (TsFk), interacts with haptenized ‘antigen-presenting cells’ (‘APC’) of both parental haplotypes, and may be described as showing dua…