Search results for "HAp"

Development of functionalized SYBR green II related cyanine dyes for viral RNA detection

2020

Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements of RNA, are unavailable. Herein we developed cyanine dyes that can be conjugated to viral capsid or other targets. First, we solved the crystal structure of SYBR Green II. The structural elucidation of this commonly used RNA probe provided the basis for synthesizing similar molecules with much desired function for post-synthetic conjugation. To address this need, cyanine dyes were prepared using an alternative synthesis protocol. All studied compounds showed considerabl…

Selective Fatty Acid Retention and Turnover in the Freshwater Amphipod Pallaseopsis quadrispinosa

2021

Gammarid amphipods are a crucial link connecting primary producers with secondary consumers, but little is known about their nutritional ecology. Here we asked how starvation and subsequent feeding on different nutritional quality algae influences fatty acid retention, compound-specific isotopic carbon fractionation, and biosynthesis of omega-3 and omega-6 polyunsaturated fatty acids (PUFA) in the relict gammarid amphipod Pallaseopsis quadrispinosa. The fatty acid profiles of P. quadrispinosa closely matched with those of the dietary green algae after only seven days of refeeding, whereas fatty acid patterns of P. quadrispinosa were less consistent with those of the diatom diet. This was ma…

Variation in ω-3 and ω-6 Polyunsaturated Fatty Acids Produced by Different Phytoplankton Taxa at Early and Late Growth Phase

2020

Phytoplankton synthesizes essential ω-3 and ω-6 polyunsaturated fatty acids (PUFA) for consumers in the aquatic food webs. Only certain phytoplankton taxa can synthesize eicosapentaenoic (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3), whereas all phytoplankton taxa can synthesize shorter-chain ω-3 and ω-6 PUFA. Here, we experimentally studied how the proportion, concentration (per DW and cell-specific), and production (µg FA L-1 day-1) of ω-3 and ω-6 PUFA varied among six different phytoplankton main groups (16 freshwater strains) and between exponential and stationary growth phase. EPA and DHA concentrations, as dry weight, were similar among cryptophytes and diatoms. However, Crypto…

Rahapelaaminen ja siihen liittyvä terveyskäyttäytyminen lukion ja ammattioppilaitosten 1. ja 2. vuoden opiskelijoilla

2013

Anthropometric Indicators as a Tool for Diagnosis of Obesity and Other Health Risk Factors: A Literature Review

2021

[EN] Obesity is characterized by the accumulation of an excessive amount of fat mass (FM) in the adipose tissue, subcutaneous, or inside certain organs. The risk does not lie so much in the amount of fat accumulated as in its distribution. Abdominal obesity (central or visceral) is an important risk factor for cardiovascular diseases, diabetes, and cancer, having an important role in the so-called metabolic syndrome. Therefore, it is necessary to prevent, detect, and appropriately treat obesity. The diagnosis is based on anthropometric indices that have been associated with adiposity and its distribution. Indices themselves, or a combination of some of them, conform to a big picture with di…

Changes in Fat Oxidation and Body Composition after Combined Exercise Intervention in Sedentary Obese Chinese Adults.

2022

(1) Background: Evidence suggests that aerobic exercise and high-intensity interval training (HIIT) might increase fat oxidation and reduce fat. However, limited research has examined the effects of combining progressive aerobic exercise and HIIT interventions in sedentary adults with overweight and obesity, and differences in its effects between men and women remain unclear. The purpose of this study was to investigate the effects of combined progressive aerobic exercise and HIIT (CAEH) on fat oxidation and fat reduction in sedentary Chinese adults and compare sex differences in sedentary adults after seven weeks. (2) Methods: Eighty-four sedentary obese adults were enrolled and allocated …

Backbending in the pear-shaped 223(90)Th nucleus: Evidence of a high-spin octupole to quadrupole shape transition in the actinides

2017

International audience; Relatively neutron-rich thorium isotopes lie at the heart of a nuclear region of nuclei exhibiting octupole correlation effects. The detailed level structure of Th223 has been investigated in measurements of γ radiation following the fusion-evaporation channel of the Pb208(O18,3n)Th223 reaction at 85 MeV beam energy. The level structure has been extended up to spin 49/2, and 33 new γ rays have been added using triple-γ coincidence data. The spins and parities of the newly observed states have been confirmed by angular distribution ratios. In addition to the two known yrast bands based on a K=5/2 configuration, a non-yrast band has been established up to spin 35/2. We…

Morphological characterization of neuronal and non-neuronal phenotypes of the human neuroblastoma SH-SY5Y cell line

2008

Engineering of charge, biotin-binding and oligomerization of avidin : new tools for avidin-biotin technology

2002

Avidin is a tetrameric glycoprotein found in chicken egg white. Due to its remarkably high affinity for biotin, avidin has widely been used in biological and biotechnical applications in life sciences. Avidin is also a highly stable protein, maintaining its functional tetrameric structure at high temperatures, extremes of pH, and in the presence of strong denaturants. In the present study we wanted to find out whether different properties of avidin can be modified with protein engineering using rational design. We used the 3D-structure of avidin together with sequence information from various avidin-like proteins to plan these changes. Besides shedding more light on the protein chemical bas…

Differential effects of perceptions of equal, favourable and unfavourable autonomy support on educational and well-being outcomes

2019

Abstract In this study, we examined whether high-school students experienced optimal educational and well-being outcomes when they perceived that they and their classmates received an equal, rather than unequal, and high amount of autonomy support from teachers. In a prospective study that aimed to predict academic grades and well-being outcomes, surface analyses of polynomial regression equations pointed that perceptions of equal autonomy support were the most optimal in terms of yielding highest levels of need satisfaction, autonomous forms of motivation and happiness with math courses. Additionally, in accordance with tenets of self-determination theory, we demonstrated that effects asso…