Search results for "HAp"
showing 10 items of 2767 documents
Mitochondrial DNA Regionalism and Historical Demography in the Extant Populations of Chirocephalus kerkyrensis (Branchiopoda: Anostraca)
2012
BackgroundMediterranean temporary water bodies are important reservoirs of biodiversity and host a unique assemblage of diapausing aquatic invertebrates. These environments are currently vanishing because of increasing human pressure. Chirocephalus kerkyrensis is a fairy shrimp typical of temporary water bodies in Mediterranean plain forests and has undergone a substantial decline in number of populations in recent years due to habitat loss. We assessed patterns of genetic connectivity and phylogeographic history in the seven extant populations of the species from Albania, Corfu Is. (Greece), Southern and Central Italy.Methodology/principal findingsWe analyzed sequence variation at two mito…
Finding Prediction Limits for a Future Number of Failures in the Prescribed Time Interval under Parametric Uncertainty
2012
Computing prediction intervals is an important part of the forecasting process intended to indicate the likely uncertainty in point forecasts. Prediction intervals for future order statistics are widely used for reliability problems and other related problems. In this paper, we present an accurate procedure, called ‘within-sample prediction of order statistics', to obtain prediction limits for the number of failures that will be observed in a future inspection of a sample of units, based only on the results of the first in-service inspection of the same sample. The failure-time of such units is modeled with a two-parameter Weibull distribution indexed by scale and shape parameters β and δ, …
Trial Methods for Nonlinear Bernoulli Problem
1997
In this article we consider a free boundary problem which is related to formation of waves on a fluid surface (for example the ship waves). We study the possibility to construct ‘trial’ methods where one solves a sequence of standard flow problems formulated in different geometries that converge to the final free boundary. Furthermore, we use the shape optimization techniques to analyse the convergence of the fixed point iteration near a fixed point. For stream function case we conclude that the fast convergence can be obtained by using non-standard boundary conditions and we present numerical results to confirm the analysis.
The Datafication of Hate: Expectations and Challenges in Automated Hate Speech Monitoring.
2020
Laaksonen, S-M.; Haapoja, J.; Kinnunen, T., Nelimarkka, M. & Pöyhtäri, R. (2020, accepted). . Frontiers in Big Data: Data Mining and Management / Critical Data and Algorithm Studies. doi:10.3389/fdata.2020.00003 Hate speech has been identified as a pressing problem in society and several automated approaches have been designed to detect and prevent it. This paper reports and reflects upon an action research setting consisting of multi-organizational collaboration conducted during Finnish municipal elections in 2017, wherein a technical infrastructure was designed to automatically monitor candidates' social media updates for hate speech. The setting allowed us to engage in a 2-fold investiga…
A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten
2017
he development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with I…
Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs.
2021
Spirotetramat is employed worldwide to fight insect pests due to its high efficiency. This chemical is quickly metabolized by plants into spirotetramat-enol, so current regulations establish that both compounds must be determined in foodstuffs for monitoring purposes. Nowadays, immunochemical methods constitute rapid and cost-effective strategies for chemical contaminant analysis at trace levels. However, high-affinity binders and suitable bioconjugates are required. In this study, haptens with opposite functionalisation sites were synthesized in order to generate high-affinity monoclonal antibodies. A direct competitive enzyme-linked immunosorbent assay with an IC50 value for the sum of sp…
Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad
2018
Nowadays, instrumental methodologies and rapid bioanalytical techniques complement each other for the analysis of toxic chemical compounds. Fluxapyroxad was commercialized a few years ago as a fungicide and today it is being used worldwide to control a variety of pests. In the present study, the development of monoclonal antibody-based immunochemical methods for the analysis of this chemical in food samples was evaluated for the first time. Novel haptens were synthesized and protein bioconjugates were prepared. High-affinity and specific monoclonal antibodies to fluxapyroxad were generated from two haptens with alternative linker tethering sites. Haptens with linker site heterology and a st…
Mammal assemblage composition predicts global patterns in emerging infectious disease risk
2021
Abstract As a source of emerging infectious diseases, wildlife assemblages (and related spatial patterns) must be quantitatively assessed to help identify high‐risk locations. Previous assessments have largely focussed on the distributions of individual species; however, transmission dynamics are expected to depend on assemblage composition. Moreover, disease–diversity relationships have mainly been studied in the context of species loss, but assemblage composition and disease risk (e.g. infection prevalence in wildlife assemblages) can change without extinction. Based on the predicted distributions and abundances of 4466 mammal species, we estimated global patterns of disease risk through …
Phytoplankton distribution along trophic gradients within and among reservoirs in Catalonia (Spain)
2008
SUMMARY 1. Longitudinal gradients in the epilimnetic waters of stratified reservoirs provide a useful database to study changing environmental conditions. The spatial distribution, assemblage structure and specific adaptations of phytoplankton assemblages can be analysed along these gradients over short time scales. 2. Four reservoirs with a similar typology, located along an altitudinal gradient in the same eco-region, were sampled along their longitudinal axes. In total, 19 sampling stations provided a trophic spectrum, ranging from oligo-mesotrophy to hypertrophy, which was quantified by calculating the trophic state index of each sampling station in the four reservoirs. 3. Several patte…
Trehalose effects on α-crystallin aggregates
2007
alpha-Crystallin in its native state is a large, heterogeneous, low-molecular weight (LMW) aggregate that under certain conditions may progressively became part of insoluble high-molecular weight (HMW) systems. These systems are supposed to play a relevant role in eye lens opacification and vision impairment. In this paper, we report the effects of trehalose on alpha-crystallin aggregates. The role of trehalose in alpha-crystallin stress tolerance, chaperone activity and thermal stability is studied. The results show that trehalose stabilizes the alpha-crystallin native structure, inhibits alpha-crystallin aggregation, and disaggregates preformed LMW systems not affecting its chaperone acti…