Search results for "HEMOGLOBIN"

The Measurement of the CO2 Hb Binding Curve of Human Hemoglobin by 13C-NMR Spectrometry

1980

It is well known that under physiological conditions CO2 binds to the four terminal amino groups of hemoglobin (Hb) (Kilmartin and Rossi-Bernardi, 1971). The carbamino compounds which are formed by this reaction are involved in the Haldane effect.

The Root effect—a physiological perspective

2004

Reaction of melatonin with hemoglobin-derived oxoferryl radicals and inhibition of the hydroperoxide-induced hemoglobin denaturation in red blood cel…

2001

Melatonin has been shown to act as a radical scavenger in various chemical and biological model systems in vitro. Kinetic evidence is now provided showing that melatonin inhibits the irreversible degradation of hemoglobin (Hb), when incubated with red blood cells exposed to the oxidant activity of cumene hydroperoxide (cumOOH). A decrease of heme loss and accumulation of soluble methemoglobin (met-Hb) are explained in terms of the interaction of the indoleamine with perferryl Hb ( . Hb[Fe IV = O]), a highly reactive Hb-derived radical species responsible for the irreversible Hb degradation. A kinetic study, in pure chemical solution, showed that melatonin can effectively reduce the oxoferry…

An electrospray ionization mass spectrometric study of the extracellular hemoglobins from Chironomus thummi thummi.

1998

The aquatic larvae of the dipteran, Chironomus thummi thummi contain extracellular hemoglobins which exhibit stage-specific expression. We have used maximum entropy-based deconvolution of the complex, multiply charged electrospray ionization mass spectra, to demonstrate the presence of more than 20 components, ranging in mass from 14,417.3 Da to 17,356.5 Da in the 4th instar larvae. Of the 15 major peaks with intensities > 10 relative to 100 for the 14,417.3 Da-component (CTT-IV), only the 15,528.2-Da peak does not correspond to a known amino acid sequence. Since the number of C. thummi thummi globin genes now stands at 27, including one cDNA and not counting three that must encode known gl…

Alkaline haematin D-575, a new tool for the determination of haemoglobin as an alternative to the cyanhaemiglobin method. I. description of the method

1984

A new method for the rapid and accurate measurement of haemoglobin has been developed as an alternative to the conventional cyanhaemiglobin method. This method is based on the conversion of all haeme, haemoglobin, and haemiglobin species into a stable end product by an alkaline solution of a non-ionic detergent ('AHD reagent'). The reaction product, designated as alkaline haematin D-575, is extremely stable and shows a characteristic absorption peak at 575 nm. As compared to the cyanhaemiglobin method, the determination of haemoglobin by alkaline haematin D-575 offers several advantages such as (1) extreme stability of the AHD reagent and the conversion product, (2) decreased conversion tim…

Photometric Determination of the O2 Status of Human Blood Using the Oxystat System: cO2 (mL/dL), sO2 (%), cHb (g/dL)

1994

The O2 status of arterial human blood (Zander, 1990) can be completely described by a set of four parameters: cO2 (mL/dL), sO2 (%), cHb (g/dL) and pO2 (mm Hg).

Digital holographic microscopy as a screening technology for diabetes

2019

Label-free quantitative phase imaging (QPI) is the hallmark of digital holographic microscopy (DHM). One of the most interesting medical applications of QPI-DHM is that it can be used to analyze illnesses in which the refractive index or/and the morphology of cells/tissues are distorted, from the acquisition of a single image. In this contribution, we obtain the phase maps of red blood cells (RBCs) samples of patients suffering from diabetes mellitus type 1 (DM1) by using a DHM. Our experimental results show that the measured phase values are significantly different between control non-diabetic and diabetic patients. The high correlation coefficient between the phase and the glycated hemogl…

Immigration and changes in the epidemiology of hemoglobin disorders in Italy : an emerging public health burden

2012

Abstract Background In the last years Italy is confronting with massive migratory movements from developing countries where hemoglobinopathies are widespread. This is causing a large diffusion and a changing spectrum in the epidemiology of hemoglobin disorders in Italy. Methods Investigations recently published in Italy on hemoglobinopathies among immigrants were revised in order to appreciate the impact of immigration from developing countries on epidemiology of these pathologies and to outline adequate guidelines of prevention. Results Although in Italy there is a limited number of investigations regarding the relation between immigration and hemoglobin disorders, published data show that…

Hemoconcentration induced by exercise: Revisiting the Dill and Costill equation

2014

The Dill and Costill equation is used to estimate the exercise-induced hemoconcentration. However, this cal- culation requires drawing an extra whole-blood sample, which cannot be frozen and has to be analyzed with dedi- cate instrumentation in a relative short time. The aim of the present study was to explore the usefulness of some serum biochemical parameters to estimate hemoconcen- tration induced by exhaustive exercise. Fourteen healthy male subjects (19-34 years) performed a15-min running test at 110% of anaerobic threshold speed. Hemoglobin, hematocrit, brain natriuretic peptide (BNP), creatinine, gamma-glutamyltransferase (GGT), total-proteins, albu- min, total calcium (Ca), K + ,N a…

Quantitative corneal anatomy: evaluation of the effect of diabetes duration on the endothelial cell density and corneal thickness.

2014

Purpose To evaluate the differences in endothelial cell density (ECD) and central corneal thickness (CCT) between type II diabetic patients and age-matched healthy controls, and determine the impact of time from diagnosis. Methods This is a comparative study of 77 eyes of type II diabetic patients (33 males, 44 females) and 80 eyes of healthy subjects (42 males, and 38 females) whose ages ranged from 38 to 56 years. CCT, ECD, HbA1c levels, and Goldmann tonometry were measured. Results The CCT was significantly higher and the ECD significantly lower in long-term diabetic patients (10 years + since diagnosis) when compared with short-term diabetic patients (<1 year since diagnosis) and contro…