Search results for "HL-60"

showing 3 items of 43 documents

Enzymatically modified, nonoxidized LDL induces selective adhesion and transmigration of monocytes and T-lymphocytes through human endothelial cell m…

1999

Abstract —Circulating monocytes and T lymphocytes extravasate through the endothelium at sites of developing atheromatous lesions, where they tend to accumulate and mediate the progression of the disease. We have previously demonstrated the presence of an enzymatically degraded, nonoxidized form of LDL (E-LDL) in early human fatty streaks, which possesses major biological properties of an atherogenic lipoprotein. The effects of E-LDL on human endothelial cells have now been studied with respect to adhesion and transmigration of monocytes and T lymphocytes. E-LDL induced a rapid and dose-dependent selective adhesion of monocytes and T lymphocytes to endothelial cell monolayers within 30 min…

Umbilical VeinsP-selectinArteriosclerosisT-LymphocytesIntercellular Adhesion Molecule-1HL-60 CellsBiologyMonocytesMuscle Smooth VascularCell MovementE-selectinmedicineCell AdhesionHumansLymphocyte homing receptorCell adhesionDose-Response Relationship DrugMonocyteT lymphocyteCholesterol LDLIntercellular Adhesion Molecule-1Molecular biologyEndothelial stem cellLipoproteins LDLPlatelet Endothelial Cell Adhesion Molecule-1KineticsP-Selectinmedicine.anatomical_structureImmunologybiology.proteinlipids (amino acids peptides and proteins)Endothelium VascularCardiology and Cardiovascular MedicineE-SelectinArteriosclerosis, thrombosis, and vascular biology
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Supramolecular Association of Halochromic Switches and Halloysite Nanotubes in Fluorescent Nanoprobes for Tumor Detection

2022

Fluorescence imaging has become an indispensable tool in the biomedical laboratory to elucidate the fundamental dynamic and structural factors regulating cellular processes. The development of fluorescent nanoprobes represents a challenge to detect any cellular process under a microscope. Herein, a fluorescent nanomaterial was synthesized by exploiting the supramolecular interaction between a halochromic switch (1Cl) and halloysite nanotubes (HNTs). The successful synthesis of a HNTs/1Cl nanomaterial was confirmed by thermogravimetric analysis and Fourier transform infrared. The aqueous mobility was investigated by dynamic light scattering and ζ-potential measurements as well. Furthermore, …

fluorescent probesMCF-7 cell linesSettore BIO/14 - FarmacologiaHL-60R cell linesGeneral Materials Sciencehalloysite nanotubesSettore CHIM/06 - Chimica Organicatumor detectionhalochromic switchessupramolecular interactionsSettore CHIM/12 - Chimica Dell'Ambiente E Dei Beni CulturaliSettore CHIM/02 - Chimica Fisica
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No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI.

2003

The purpose of this study was to examine whether exposure to magnetic fields (MFs) relevant for magnetic resonance imaging (MRI) in clinical routine influences cell cycle progression in two tumor cell lines in vitro. HL60 and EA2 cells were exposed to four types of MFs: (i) static MF of 1.5 and 7.05 T, (ii) extremely low frequency magnetic gradient fields (ELFMGFs) with ± 10 mT/m and 100 Hz, as well as ± 100 mT/m and 100 Hz, (iii) pulsed high frequency MF in the radiofrequency (RF) range (63.6 MHz, 5.8 μT), and (iv) a combination of (i–iii). Exposure periods ranged from 1 to 24 h. Cell cycle distribution (G0/G1, S, and G2/M phases) was analyzed by flow cytometry. Cell cycle analysis did not…

medicine.diagnostic_testPhysiologyChemistryCell CycleBiophysicsMagnetic resonance imagingDose-Response Relationship RadiationHL-60 CellsGeneral MedicineEnvironmental ExposureCell cycleMagnetostaticsRadiation DosageMagnetic Resonance ImagingFlow cytometryNuclear magnetic resonanceElectromagnetic FieldsCell culturemedicineHumansRadiology Nuclear Medicine and imagingExtremely low frequencyIrradiationRadiometryBioelectromagneticsBioelectromagnetics
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