Search results for "HLA-DR"

showing 10 items of 96 documents

The influence of major histocompatibility complex class II genes and T-cell Vbeta repertoire on response to immunization with HBsAg.

1998

Nonresponsiveness to HBsAg vaccination is observed in 5-10% of vaccine recipients and is possibly caused by a defect in the T helper cell compartment. The immune response to HBsAg is influenced by genes of the major histocompatibility complex. We have investigated MHC class I and class II antigens in 53 adult responders and 73 nonresponders. Results obtained in this first study were tested in a second study with 56 responders and 62 nonresponders from an infant vaccination trial. In addition, the peripheral Vbeta-chain T-cell receptor repertoire was investigated using monoclonal antibodies and flow-cytometry in 26 adult responders and 38 nonresponders. As previously reported, nonresponsiven…

AdultHBsAgT cellReceptors Antigen T-Cell alpha-betaImmunologyGenes MHC Class IIMajor histocompatibility complexCohort StudiesImmune systemGene FrequencyMHC class ImedicineImmunology and AllergyHumansHepatitis B VaccinesAllelesDiphtheria-Tetanus-Pertussis VaccineHepatitis B Surface AntigensbiologyT-cell receptorInfantGeneral MedicineT helper cellHLA-DR AntigensVirologyVaccinationmedicine.anatomical_structureImmunologybiology.proteinImmunizationHLA-DRB1 ChainsHuman immunology
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HLA-DRB1*1301 AND *1302 protect against chronic hepatitis B

1997

Abstract Background/Aims: The outcome of acute hepatitis B infection may be influenced by host factors like the major histocompatibility complex (MHC). We have investigated MHC class I and class II antigens in patients with chronic hepatitis B compared to a healthy control population. To confirm the findings of this first study we performed a second study in a group of subjects who had spontaneously recovered from acute hepatitis B infection. Methods: Frequencies of MHC class I and class II antigens were analyzed in patients with chronic hepatitis B virus infection and in control subjects. MHC class I typing was done by standard microlymphocytotoxicity assays. DRB1 and DQA1 genotypes were d…

AdultHepatitis B virusRemission SpontaneousPopulationEnzyme-Linked Immunosorbent AssayMajor histocompatibility complexmedicine.disease_causePolymerase Chain ReactionHLA-DQ alpha-ChainsVirusHLA-DQ AntigensMHC class ImedicineHumansSerologic TestsProspective StudiesHepatitis B AntibodieseducationHLA-DRB1AllelesHepatitis B viruseducation.field_of_studyMHC class IIHepatitis B Surface AntigensHepatologybiologyHLA-DR AntigensHepatitis BVirologyChronic infectionImmunoglobulin GChronic DiseaseDNA ViralImmunologybiology.proteinHLA-DRB1 ChainsJournal of Hepatology
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Correlation of Clinical and Immunologic Parameters of the Inflammatory Activity of Pulmonary Sarcoidosis

1991

The evaluation of activation markers such as T4/T8 ratio and HLA-DR expression of lymphocytes of bronchoalveolar lavage (L-BAL) is an important clinical approach for the staging of sarcoidosis. However, it is not known to what extent this is paralleled by an exaggerated lymphocyte function. We investigated the dependence of L-BAL activation markers on the production of interleukin-2 (IL-2) by L-BAL and on the soluble IL-2 receptor serum level (sIL-2R) in 116 patients with sarcoidosis. In none of the combinations tested was a correlation between the two groups of parameters found; r less than 0.5, upper 90% confidence limit of r less than 0.8. Interestingly, IL-2 production is independent of…

AdultLung DiseasesMalePulmonary and Respiratory MedicineSystemic diseaseSarcoidosisT-LymphocytesLymphocyteCD4-CD8 RatioCD4-CD8 RatioEnzyme-Linked Immunosorbent AssayInflammationLymphocyte ActivationHumansMedicineReceptormedicine.diagnostic_testbusiness.industryRespiratory diseaseReceptors Interleukin-2HLA-DR Antigensrespiratory systemmedicine.diseaserespiratory tract diseasesBronchoalveolar lavagemedicine.anatomical_structureImmunologyInterleukin-2FemaleSarcoidosismedicine.symptombusinessBronchoalveolar Lavage FluidAmerican Review of Respiratory Disease
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Erratum to: Donor age and long-term culture do not negatively influence the stem potential of limbal fibroblast-like stem cells

2016

In regenerative medicine the maintenance of stem cell properties is of crucial importance. Ageing is considered a cause of reduced stemness capability. The limbus is a stem niche of easy access and harbors two stem cell populations: epithelial stem cells and fibroblast-like stem cells. Our aim was to investigate whether donor age and/or long-term culture have any influence on stem cell marker expression and the profiles in the fibroblast-like stem cell population.Fibroblast-like stem cells were isolated and digested from 25 limbus samples of normal human corneo-scleral rings and long-term cultures were obtained. SSEA4 expression and sphere-forming capability were evaluated; cytofluorimetric…

AdultMale0301 basic medicineStage-Specific Embryonic AntigensPrimary Cell CultureGene ExpressionMedicine (miscellaneous)Limbus CorneaeBiologyBiochemistry Genetics and Molecular Biology (miscellaneous)Donor age03 medical and health sciencesCell MovementSpheroids CellularmedicineATP Binding Cassette Transporter Subfamily G Member 2HumansFibroblastAgedCell ProliferationStem CellsAge FactorsEpithelium CornealCell DifferentiationEpithelial CellsHLA-DR AntigensNanog Homeobox ProteinCell BiologyFibroblastsMiddle AgedMolecular medicinehumanitiesNeoplasm ProteinsCell biology030104 developmental biologymedicine.anatomical_structureLeukocyte Common AntigensMolecular MedicineFemaleErratumStem cellOctamer Transcription Factor-3BiomarkersStem Cell Research & Therapy
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Antibodies to soluble liver antigen/liver pancreas and HLA risk factors for type 1 autoimmune hepatitis.

2002

Antibodies to soluble liver antigen/liver-pancreas are highly specific markers of type 1 autoimmune hepatitis that have been associated with relapse. Our aim was to determine if these antibodies are reflective of a genetic predisposition for recrudescent disease.One hundred forty-four white North American patients were evaluated by an enzyme immunoassay and by Western blot using recombinant soluble liver antigen/liver-pancreas; 122 were assessed for class II human leukocyte antigens (HLAs).Twenty-two patients (15%) had antibodies to soluble liver antigen/liver-pancreas. These patients were indistinguishable from seronegative patients by clinical, laboratory, and histological features at pre…

AdultMaleAnti-nuclear antibodyBlotting WesternEnzyme-Linked Immunosorbent AssayAutoimmune hepatitisHuman leukocyte antigenAutoantigensRisk AssessmentSensitivity and SpecificityStatistics NonparametricHLA-DR3 AntigenRisk FactorsmedicineHLA-DR4 AntigenHumansProbabilityAutoimmune diseaseHepatitisHepatologybiologybusiness.industryGastroenterologyPanel reactive antibodyPancreatic DiseasesMiddle Agedmedicine.diseaseHepatitis Autoimmunemedicine.anatomical_structureSolubilityAntibodies AntinuclearImmunologybiology.proteinDrug Therapy CombinationFemaleAntibodyPancreasbusinessThe American journal of gastroenterology
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Clinical significance of autoantibodies to soluble liver antigen in autoimmune hepatitis.

1999

Abstract Background/Aims: Classification of autoimmune hepatitis (AIH) into different subgroups according to autoantibody status has been proposed: type I (ANA/SMA), type II (LKM-1) and type III (anti-SLA). However, whether type III AIH forms a clinically distinct disease entity remains controversial. The aim of this study was to evaluate the subclassification of AIH into ANA/SMA and anti-SLA positive patients with regard to clinical, biochemical and histologic differences. Methods: Ninety-seven consecutive patients with a well-documented long-term course of AIH with ANA/SMA and/or anti-SLA autoantibodies were studied. Clinical, biochemical and histological features of patients with ANA/SMA…

AdultMaleAnti-nuclear antibodyHLA-DR3Autoimmune hepatitisAutoantigensAutoimmune DiseasesHepatitisimmune system diseasesmedicineHumansClinical significanceAutoantibodiesAutoimmune diseaseHepatitisImmunosuppression TherapyHepatologybusiness.industryfungiAutoantibodyMuscle SmoothMiddle Agedmedicine.diseaseSMA*PrognosisAntibodies AntinuclearImmunologyFemalebusinessJournal of hepatology
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Association of smoking but not HLA-DRB1*15:01, APOE or body mass index with brain atrophy in early multiple sclerosis

2019

Background: The course of multiple sclerosis (MS) shows substantial inter-individual variability. The underlying determinants of disease severity likely involve genetic and environmental factors. Objective: The aim of this study was to assess the impact of APOE and HLA polymorphisms as well as smoking and body mass index (BMI) in the very early MS course. Methods: Untreated patients ( n = 263) with a recent diagnosis of relapsing-remitting (RR) MS or clinically isolated syndrome underwent standardized magnetic resonance imaging (MRI). Genotyping was performed for single-nucleotide polymorphisms (SNPs) rs3135388 tagging the HLA-DRB1*15:01 haplotype and rs7412 (Ɛ2) and rs429358 (Ɛ4) in APOE. …

AdultMaleApolipoprotein EMultiple SclerosisAdolescentPolymorphism Single NucleotideBody Mass IndexYoung Adult03 medical and health sciencesApolipoproteins E0302 clinical medicineAtrophyMedizinische FakultätmedicineHumansSNPGenetic Predisposition to Disease030212 general & internal medicineddc:610Risk factorHLA-DRB1Agedbusiness.industryMultiple sclerosisSmokingNeurodegenerationBrainMiddle Agedmedicine.diseaseNeurologyImmunologyFemaleNeurology (clinical)AtrophybusinessBody mass index030217 neurology & neurosurgeryHLA-DRB1 Chains
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C4A deficiency and nonresponse to hepatitis B vaccination

2002

Hepatitis B vaccination failure has been linked to the presence of certain human leukocyte antigen class II alleles. However, the functional background of these associations has remained unclear. Complement component C 4 is encoded within the major histocompatibility complex and is essential for classical pathway activation.Healthy individuals (n=4269) were vaccinated in a prospective trial with Engerix B. Nonresponse was classified as anti-HBs10 U/l after the last vaccination. Seventy-three nonresponders (NR) (1.7%) were identified. For comparison 53 responders (R) (anti-HBs10 IU/l) were drawn randomly from the same cohort. C4 allotyping was carried out by high-voltage agarose gel electrop…

AdultMaleBiologyMajor histocompatibility complexClassical complement pathwaySeroepidemiologic StudiesHumansHepatitis B VaccinesProspective StudiesTreatment FailureHepatitis B AntibodiesSouthern blotGel electrophoresisB-LymphocytesHepatologyHaplotypeComplement C4aHLA-DR AntigensMiddle AgedHepatitis BVirologyComplement systemLogistic ModelsHaplotypesAgarose gel electrophoresisImmunologybiology.proteinFemaleVaccine failureGene DeletionHLA-DRB1 ChainsJournal of Hepatology
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Pathogenesis of autoimmune diseases associated with 8.1 ancestral haplotype: a genetically determined defect of C4 influences immunological parameter…

2003

Abstract Subjects with certain HLA alleles have a higher risk of specific autoimmune diseases than those without these alleles. The 8.1 ancestral haplotype (AH) is a common Caucasoid haplotype carried by most people who type for HLA-B8,DR3. It is unique in its association with a wide range of immunopathological diseases. To gain insight into the identification of the mechanism(s) of disease susceptibility of 8.1 AH carriers, we have investigated the prevalence of circulating immune complexes and non-organ-specific autoantibodies in healthy carriers of the haplotype. The results show that carriers of 8.1 AH display both a significant increased prevalence of immune complexes and higher titers…

AdultMaleEnzyme-Linked Immunosorbent AssayHuman leukocyte antigenBiologyAutoimmune DiseasesHLA-B8 AntigenImmune systemHLA-DR3 AntigenAntigenGene FrequencyHLA AntigensGenetic predispositionmedicineHumansAlleleAllelesPharmacologyAutoimmune diseaseGeneticsHaplotypeAutoantibodyComplement C4General MedicineMiddle Agedmedicine.diseaseHaplotypesImmunologyFemaleBiomedicinepharmacotherapy = Biomedecinepharmacotherapie
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Differential genetic determination of immune responsiveness to hepatitis B surface antigen and to hepatitis A virus: a vaccination study in twins.

2002

Summary Background The course of viral hepatitis is thought to be affected by genetic host variability and, in particular, by genes of the major histocompatibility locus. Hepatitis A and B vaccination is a useful model to study the effect of host factors on the immune response to viral antigens. We aimed to assess the heritability of the HBsAg (anti-HBs) and anti-hepatitis A virus (anti-HAV) immune response and to estimate the effect of the HLA-DRB1 locus and other genetic loci unlinked to HLA. Methods We did an open prospective study and vaccinated 202 twin pairs with a combined recombinant HBsAg/inactivated hepatitis A vaccine. We measured antibodies to HBsAg and HAV and determined HLA-DR…

AdultMaleHBsAgAdolescentHepatitis A vaccineHuman leukocyte antigenBiologyHepatitis A AntibodiesmedicineHumansHepatitis B VaccinesHepatitis B AntibodiesAgedGeneticsHepatitis B Surface AntigensVaccinationvirus diseasesHepatitis AGeneral MedicineHLA-DR AntigensHeritabilityMiddle Agedmedicine.diseasebiology.organism_classificationdigestive system diseasesRecombinant ProteinsImmunity ActiveHepadnaviridaeImmunologybiology.proteinTwin Studies as TopicFemaleHepatitis A virusAntibodyViral hepatitisHLA-DRB1 ChainsLancet (London, England)
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