Search results for "HORTICULTURE"
showing 10 items of 1421 documents
Training experiments with Bombus terrestris and Apis mellifera on artificial ‘ Salvia ’ flowers
2016
Abstract Many bee flowers demand physical force from their pollinators. An example is Salvia with lever-like anthers restricting access to nectar. Though the needed force is generally low, it is largely unknown how far even low forces affect flower preference and foraging behaviour of bees. In the present paper, we introduce a method to measure the response of bees to a force mechanism. We explain the construction of artificial flowers mimicking the lever mechanism of a Salvia flower and the procedure to train honeybees and bumblebees to the models. We document the behaviour of the bees on models without barriers and on those with low forces aiming at the same time to determine the bees’ se…
Whole-genome resequencing of Cucurbita pepo morphotypes to discover genomic variants associated with morphology and horticulturally valuable traits
2019
[EN] Cucurbita pepo contains two cultivated subspecies, each of which encompasses four fruit-shape morphotypes (cultivar groups). The Pumpkin, Vegetable Marrow, Cocozelle, and Zucchini Groups are of subsp. pepo and the Acorn, Crookneck, Scallop, and Straightneck Groups are of subsp. ovifera. Recently, a de novo assembly of the C. pepo subsp. pepo Zucchini genome was published, providing insights into its evolution. To expand our knowledge of evolutionary processes within C. pepo and to identify variants associated with particular morphotypes, we performed whole-genome resequencing of seven of these eight C. pepo morphotypes. We report for the first time whole-genome resequencing of the four…
Gametic embryogenesis through isolated microspore culture in Corylus avellana L.
2015
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report…
Microspore Embryogenesis in Almond (Prunus dulcis Mill.)
2018
Almond is the most important tree nut crop in terms of commercial production. Its production is limited to areas characterized by a Mediterranean climate, including regions in the Mediterranean countries, the Central Valley of California, Central Asia, the Himalayan slopes and some equivalent areas in the Southern Hemisphere, including Argentina, Australia and South Africa (Kester et al. 1975).
Twig and Shoot Dieback of Citrus, a New Disease Caused by Colletotrichum Species
2021
(1) Background: This study was aimed at identifying the Colletotrichum species associated with twig and shoot dieback of citrus, a new syndrome occurring in the Mediterranean region and also reported as emerging in California. (2) Methods: Overall, 119 Colletotrichum isolates were characterized. They were recovered from symptomatic trees of sweet orange, mandarin and mandarin-like fruits during a survey of citrus groves in Albania and Sicily (southern Italy). (3) Results: The isolates were grouped into two distinct morphotypes. The grouping of isolates was supported by phylogenetic sequence analysis of two genetic markers, the internal transcribed spacer regions of rDNA (ITS) and β-tubulin …
Effects of plant density and cutting-type on rooting and growth of an extensive green roof of Sedum sediforme (Jacq.) Pau in a Mediterranean environm…
2020
Abstract Species of the Sedum genus are a popular choice for green roofs as they thrive in shallow growth layers and are resilient to extreme environmental conditions; they are also easy to propagate from seed, plantlings or plant parts. The use of rooted cuttings is most widespread due to good rates of establishment. In the interest of cost reduction, the direct spreading of Sedum cuttings over the roof area, especially over large areas, has attracted attention in recent years. Considering the interest on the above method and the seeming lack of experimental data available, this study aims to further knowledge in this area. Experimental tests on direct spreading of unrooted cuttings of Sed…
Stress integrated tests and cytological analyses reveal Brassica villosa subsp. drepanensis seed quality decrease upon long-term storage
2016
Under stress integrated germination test (SIGT), seeds undergo osmo-saline stresses, which enable to detect differences in vigour of long-term stored seeds with high germination percentage (G%). The quality of Brassica villosa subsp. drepanensis seeds stored in a genebank (at -20°C for 16 years) was compared with seeds at harvest by standard germination tests (GT), SIGT and cytogenetic analysis. No differences were detected in G% and mean germination time under GT. Conversely, SIGT performed with NaCl -0.9MPa osmotic potential did not influence G% at harvest but reduced that of stored seeds, SIGT at -1.4MPa reduced G% of both. Cytogenetic analysis showed reduction of mitotic index, appearan…
Effects of transgenic expression of Brevibacterium linens methionine gamma lyase (MGL) on accumulation of Tylenchulus semipenetrans and key aminoacid…
2017
Key message Carrizo transgenic plants overexpressing methionine-gamma-lyase produced dimethyl sulfide. The transgenic plants displayed more resistance to nematode attacks (Tylenculus semipenetrans) and may represent an innovative strategy for nematode control. Abstract Tylenchulus semipenetrans is a nematode pest of many citrus varieties that causes extensive damage to commercial crops worldwide. Carrizo citrange vr. (Citrus sinensis L. Usb × Poncirus trifoliate L. Raf) plants overexpressing Brevibacterium linens methionine-gamma-lyase (BlMGL) produced the sulfur volatile compound dimethyl sulfide (DMS). The aim of this work was to determine if transgenic citrus plants expressing BlMGL show…
Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions
2018
Dickeya and Pectobacterium are responsible for causing blackleg of plants and soft rot of tubers in storage and in the field, giving rise to losses in seed potato production. In an attempt to improve potato health, biocontrol activity of known and putative antagonists was screened using in vitro and in planta assays, followed by analysis of their persistence at various storage temperatures. Most antagonists had low survival on potato tuber surfaces at 4 °C. The population dynamics of the best low‐temperature tolerant strain and also the most efficient antagonist, Serratia plymuthica A30, along with Dickeya solani as target pathogen, was studied with TaqMan real‐time PCR throughout the stora…
Cultivar- and Wood Area-Dependent Metabolomic Fingerprints of Grapevine Infected by Botryosphaeria Dieback
2020
International audience; Botryosphaeria dieback is one of the most significant grapevine trunk diseases that affects the sustainability of the vineyards and provokes economic losses. The causal agents, Botryosphaeriaceae species, live in and colonize the wood of the perennial organs causing wood necrosis. Diseased vines show foliar symptoms, chlorosis, or apoplexy, associated to a characteristic brown stripe under the bark. According to the susceptibility of the cultivars, specific proteins such as PR-proteins and other defense-related proteins are accumulated in the brown stripe compared with the healthy woody tissues. In this study, we enhanced the characterization of the brown stripe and …