Search results for "HUVECs"

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Dexamethasone Inhibits the Pro-Angiogenic Potential of Primary Human Myoblasts

2021

Tissue regeneration depends on the complex processes of angiogenesis, inflammation and wound healing. Regarding muscle tissue, glucocorticoids (GCs) inhibit pro-inflammatory signalling and angiogenesis and lead to muscle atrophy. Our hypothesis is that the synthetic GC dexamethasone (dex) impairs angiogenesis leading to muscle atrophy or inhibited muscle regeneration. Therefore, this study aims to elucidate the effect of dexamethasone on HUVECs under different conditions in mono- and co-culture with myoblasts to evaluate growth behavior and dex impact with regard to muscle atrophy and muscle regeneration. Viability assays, qPCR, immunofluorescence as well as ELISAs were performed on HUVECs,…

Vascular Endothelial Growth Factor A0301 basic medicineMuscle tissueCD31endocrine systemQH301-705.5AngiogenesisMyoblasts SkeletalNeovascularization PhysiologicInflammationdexamethasonehuman primary myoblastsArticleCatalysisInorganic Chemistry03 medical and health sciences0302 clinical medicineHuman Umbilical Vein Endothelial Cellsmedicinepolycyclic compoundsHumansMyocyteBiology (General)Physical and Theoretical ChemistryQD1-999Molecular BiologySpectroscopyHUVECsTube formationChemistryOrganic ChemistryGeneral Medicineco-cultureVEGFCoculture TechniquesMuscle atrophyComputer Science ApplicationsCell biologyChemistry030104 developmental biologymedicine.anatomical_structureGene Expression RegulationCD31medicine.symptomWound healing030217 neurology & neurosurgeryhormones hormone substitutes and hormone antagonistsInternational Journal of Molecular Sciences

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