Search results for "HeLa"

showing 10 items of 738 documents

Inhibition by cellular vacuolar ATPase impairs human papillomavirus uncoating and infection.

2014

ABSTRACT Several viruses, including human papillomaviruses, depend on endosomal acidification for successful infection. Hence, the multisubunit enzyme vacuolar ATPase (V-ATPase), which is mainly responsible for endosome acidification in the cell, represents an attractive target for antiviral strategies. In the present study, we show that V-ATPase is required for human papillomavirus (HPV) infection and that uncoating/disassembly but not endocytosis is affected by V-ATPase inhibition. The infection inhibitory potencies of saliphenylhalamide, a proven V-ATPase inhibitor, and its derivatives, as well as those of other V-ATPase inhibitors, were analyzed on different HPV types in relevant cell l…

Vacuolar Proton-Translocating ATPasesEndosomeCell SurvivalCellBiologyAlphapapillomavirusEndocytosisInhibitory postsynaptic potentialAntiviral AgentsCell LineViral ProteinsmedicineHumansPharmacology (medical)Vacuolar ATPasePharmacologychemistry.chemical_classificationVacuolar Proton-Translocating ATPasesVirologyEndocytosisCell biologyInfectious Diseasesmedicine.anatomical_structureEnzymechemistryCell cultureHeLa CellsAntimicrobial agents and chemotherapy

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Hypoxia-stimulated expression of angiogenic growth factors in cervical cancer cells and cervical cancer-derived fibroblasts

2001

It is generally accepted that local growth of solid tumors and their ability to establish distant metastases are dependent on the formation of new blood vessels arising from preexisting ones (angiogenesis). The angiogenic response of the host is mediated by angiogenic molecules that are released from cancer and normal stroma cells, especially fibroblasts. The goal of the present study was to quantitatively compare the expression of the two most important angiogenic growth factors (VEGF, angiogenin) of cervical cancer cells (HeLa and Me-180) with that of cervical cancer-derived fibroblasts (from one tumor/patient) under defined normoxic and hypoxic conditions in vitro. The growth kinetics of…

Vascular Endothelial Growth Factor APathologymedicine.medical_specialtyStromal cellAngiogeninAngiogenesismedicine.medical_treatmentCellUterine Cervical NeoplasmsEnzyme-Linked Immunosorbent AssayEndothelial Growth FactorsHeLamedicineHumansHypoxiaLymphokinesNeovascularization PathologicbiologyVascular Endothelial Growth FactorsGrowth factorObstetrics and GynecologyRibonuclease PancreaticFibroblastsbiology.organism_classificationIn vitroGene Expression Regulation NeoplasticKineticsmedicine.anatomical_structureOncologyCell cultureCancer researchFemaleCell DivisionHeLa CellsInternational Journal of Gynecological Cancer

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Signal sequences modulate the immunogenic performance of human hepatitis C virus E2 gene

2005

Abstract Envelope protein E2 of human hepatitis C virus (HCV) is an attractive component of a prototype HCV vaccine. Delivered by DNA immunogens, E2 evokes specific immune response of Th1-type, failing to induce either considerable antibody production, or T-helper cell proliferation. We aimed at modulating the immunogenic performance of E2 gene by changing the mode of protein expression in eukaryotic cells. Plasmids were constructed encoding full-length E2 and nonstructural protein 1 (p7) fused to either 13 or 38 C-terminal amino acids (aa) of HCV E1 that contain second hydrophobic segment of E1 stop-transfer signal, or a complete E1 stop-transfer signal with duplicated second hydrophobic s…

Viral Hepatitis VaccinesSignal peptideGenes ViralMolecular Sequence DataImmunologyHeterologousHepacivirusProtein Sorting SignalsBiologyInjections IntramuscularEpitopeMiceViral ProteinsPlasmidViral Envelope ProteinsChlorocebus aethiopsEscherichia coliAnimalsHumansAmino Acid SequenceMolecular BiologyGeneCellular localizationCell Line TransformedMice Inbred BALB CImmunogenicityGenetic VariationCell Transformation ViralMolecular biologyCOS Cellsbiology.proteinAntibodyHeLa CellsPlasmidsMolecular Immunology

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Efficient, non-toxic anion transport by synthetic carriers in cells and epithelia.

2016

Transmembrane anion transporters (anionophores) have potential for new modes of biological activity, including therapeutic applications. In particular they might replace the activity of defective anion channels in conditions such as cystic fibrosis. However, data on the biological effects of anionophores are scarce, and it remains uncertain whether such molecules are fundamentally toxic. Here, we report a biological study of an extensive series of powerful anion carriers. Fifteen anionophores were assayed in single cells by monitoring anion transport in real time through fluorescence emission from halide-sensitive yellow fluorescent protein. A bis-(p-nitrophenyl)ureidodecalin shows especial…

Yellow fluorescent proteinpotencyGeneral Chemical Engineeringsynthetic anion carriersCystic Fibrosis Transmembrane Conductance Regulator01 natural sciencesMadin Darby Canine Kidney CellsCell membranedeliverabilityta116Drug CarriersbiologyMolecular StructureChemistryBiological activitypersistenceCystic fibrosis transmembrane conductance regulatorTransmembrane proteinanionophoresmedicine.anatomical_structureBiochemistryPhosphatidylcholinesSteroidsChlorineAnionsCell SurvivalNaphthalenesta3111010402 general chemistryDogsBacterial ProteinsCyclohexanesmedicineAnimalsHumansIon transporterCell ProliferationIon Transport010405 organic chemistryCell MembranetoxicityTransporterEpithelial CellsHydrogen BondingGeneral ChemistryRats Inbred F3440104 chemical sciencesElectrophysiological PhenomenaLuminescent ProteinsMicroscopy FluorescenceCell cultureDrug Designbiology.proteinHeLa CellsNature chemistry

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Synthesis of bis(diphenylphosphinocyclopentadienyl) yttrium chloride complexes and heterodimetallic derivatives. X-ray structure of bis[(μ-chloro)bis…

1996

Abstract Reaction of lithium diphenylphosphinocyclopentadienide with YCl 3 or YCl 3 (THF) 3 and working lead to the formation of three yttrocene phosphines: the lithium metal adduct isolated as (Ph 2 PC 5 H 4 ) 2 Y(μ-Cl) 2 Li(THF) 2 · 0.5 LiCl ( 1 ), the chloride-bridged dimeric species {(Ph 2 PC 5 H 4 ) 2 Y(μ-Cl)} 2 ( 2 ), and the coordinated monometal species [(Ph 2 PC 5 H 4 ) 2 YCl(THF)] ( 3 ). The X-ray structure of 2 is remarkable in that the crystal exhibits two independent chloride-bridged dimers that differ in the arrangement ( syn, anti ) of the diphenylphosphino groups. Chelation of phosphorus atoms to a molydenum carbonyl moiety is also reported.