Search results for "Hemichrome"

showing 2 items of 2 documents

Exposure to malondialdehyde induces an early redox unbalance preceding membrane toxicity in human erythrocytes.

2002

This work investigated the oxidative injury to human red blood cells (RBCs) by the exposure to exogenous malondialdehyde (MDA), in a physiological environment. When a 10% RBC suspension was incubated in autologous plasma, in the presence of 50 microM MDA, 30% of MDA entered into the cells. A time-course study showed that MDA caused early (30-120 min) and delayed (3-18 h) effects. MDA caused a fast depletion of reduced glutathione, and loss of the glucose-6-phosphate dehydrogenase activity, followed by a decrease of HbO2. Accumulation of methemoglobin, and formation of small amounts of hemichrome were later evident. Also, an HbO2-derived fluorescent product was measured in the membrane. The …

ErythrocytesTime FactorsOxidative phosphorylationmedicine.disease_causeBiochemistryHemolysisMethemoglobinchemistry.chemical_compoundHemoglobinsMalondialdehydemedicineHumansMethemoglobinHemichromeCell MembraneErythrocyte MembraneGeneral MedicineGlutathionemedicine.diseaseMalondialdehydeMolecular biologyHemolysisOxygenSpectrometry FluorescencechemistryBiochemistryGlucose-6-PhosphatasePotassiumElectrophoresis Polyacrylamide GelOxidation-ReductionIntracellularOxidative stressFree radical research
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Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes

2020

Extracellular vesicles (EVs) released by different cell types play an important role in many physiological and pathophysiological processes. In physiological conditions, red blood cell (RBC)-derived EVs compose 4&ndash

0301 basic medicinePhysiologyClinical BiochemistryCell<i>tert-</i>Bytyl hydroperoxide t-BOOHmedicine.disease_causeBiochemistryband 3ArticleFlow cytometry03 medical and health sciences0302 clinical medicinecalcium ionophore A23187medicineoxidative stressViability assay<i>tert</i>-Bytyl hydroperoxide t-BOOHnitric oxide donorMolecular BiologyBand 3Hemichromemicroparticlesbiologymedicine.diagnostic_testChemistryMicrovesiclelcsh:RM1-950Cell Biologytert-Bytyl hydroperoxide t-BOOHvesiculationRed blood cell030104 developmental biologymedicine.anatomical_structurelcsh:Therapeutics. Pharmacology030220 oncology & carcinogenesisphysiologybiology.proteinBiophysicserythrocytesOxidative stressAntioxidants
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