Search results for "Hepatitis B e Antigen"

showing 10 items of 43 documents

Proliferative response of CD4+ T cells and hepatitis B virus clearance in chronic hepatitis with or without hepatitis B e-minus hepatitis B virus mut…

1995

To assess the significance of cell-mediated immunity, T cells were derived from the peripheral blood and liver tissue of hepatitis B virus (HBV)-infected patients and controls. The analysis of the 3H-thymidine-uptake in response to a panel of recombinant HBV antigens revealed that peripheral blood mononuclear cells (PBMC) of the 25 viremic patients with inflammatory active, chronic hepatitis B, 16 with wild-type and nine with HBe-minus HBV mutant infection, showed stronger proliferative responses to HBc and HBe antigens than 16 asymptomatic nonviremic HBsAg carriers with normal aminotransferase levels (HBc: SI 19.3 +/- 3.9 vs. 13.0 +/- 3.2 vs. 8.0 +/- 1.2; P.01 and HBe: SI 16.6 +/- 4.0 vs. …

CD4-Positive T-LymphocytesHBsAgHepatitis B virusmedicine.disease_causeVirusAntigenmedicineHumansHepatitis B e AntigensHepatitis B virusHepatologybiologybusiness.industryvirus diseasesInterferon-alphaHepatitis Bmedicine.diseasebiology.organism_classificationHepatitis BVirologyHepatitis B Core Antigensdigestive system diseasesHBcAgHBeAgHepadnaviridaeImmunologyChronic DiseaseMutationbusinessCell DivisionHepatology (Baltimore, Md.)
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Naïve hepatitis B e antigen-negative chronic hepatitis B patients are at risk of carotid atherosclerosis: A prospective study

2021

BACKGROUND There is an increased risk of atherosclerosis in patients with chronic hepatitis C or human immunodeficiency virus, but there is scarce data on hepatitis B virus infection. The hypothesis of this study is that hepatitis B virus infection increases the risk of carotid plaques and subclinical atherosclerosis in naïve hepatitis B e antigen (HBeAg) negative subjects. AIM To assess the rate of carotid plaques and subclinical atherosclerosis in naïve HBeAg negative subjects in comparison with a cohort of healthy controls. METHODS Prospective case-control collaborative study conducted in two tertiary hospitals. Four hundred and two subjects prospectively recruited at the outpatient clin…

Carotid Artery DiseasesHepatitis B virusmedicine.medical_specialtyIntima-media thicknessCardiovascular risk Carotid plaques Endothelial dysfunction Hepatitis B virus Intima-media thickness Subclinical atherosclerosis Carotid Intima-Media Thickness Hepatitis B e Antigens Humans Prospective Studies Carotid Artery Diseases Hepatitis B Chronicmedicine.disease_causeCarotid Intima-Media ThicknessGastroenterologyHepatitis B ChronicInternal medicinemedicineHumansOutpatient clinicCarotid plaquesSubclinical atherosclerosisEndothelial dysfunctionHepatitis B e AntigensProspective StudiesRisk factorProspective cohort studyHepatitis B virusbusiness.industryGastroenterologyGeneral MedicineOdds ratioHepatitis BCardiovascular riskmedicine.diseaseIntima-media thicknessHBeAgProspective StudybusinessWorld Journal of Gastroenterology
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Low frequency of cytotoxic liver-infiltrating T lymphocytes specific for endogenous processed surface and core proteins in chronic hepatitis B.

1993

To investigate the role of hepatitis B virus (HBV)-specific CD8+ T cells in chronic hepatitis B, the lytic activity of peripheral blood mononuclear cells (PBMC) and liver-infiltrating T cell clones and cytotoxic T cell (CTL) lines stimulated by recombinant vaccinia virus-infected cells were analyzed. Autologous and allogeneic Epstein-Barr virus-transformed B cells infected with vaccinia vectors (VAC) that contain sequences of the surface (S), secretory core (E), cytoplasmatic core (C) VAC antigen of HBV, or the wild-type (WT) VAC served as target cells. ELISA and immunoblotting showed HBV antigen expression in infected cells. Neither PBMC nor C- or E-VAC-stimulated CTL lines showed specific…

Cytotoxicity ImmunologicHerpesvirus 4 HumanT cellGenes MHC Class IVaccinia virusBiologymedicine.disease_causeHepatitis B AntigensAntigenCell MovementmedicineImmunology and AllergyCytotoxic T cellHumansHepatitis B e AntigensHepatitis ChronicHepatitis B virusHepatitisB-LymphocytesHepatitis B Surface AntigensHepatitis Bmedicine.diseasebiology.organism_classificationCell Transformation ViralHepatitis BVirologyHepatitis B Core AntigensRecombinant ProteinsCTL*Infectious Diseasesmedicine.anatomical_structureHepadnaviridaeLiverProtein Processing Post-TranslationalT-Lymphocytes CytotoxicThe Journal of infectious diseases
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Cellular cytotoxicity against autologous hepatocytes in children with different forms of chronic hepatitis B.

1990

Cell-mediated immune reactions play the most important role in the pathogenesis of chronic viral and auto-immune hepatitis. Cellular cytotoxicity (CC) of peripheral blood lymphocytes against autologous hepatocytes isolated from liver biopsies was studied in 29 children with different types of hepatitis B surface antigen (HBsAg)-positive hepatitis. Children with chronic hepatitis B showed higher cytotoxicity than control patients. However, a correlation of cytotoxicity to serum amino-transferases, HBeAg-/Anti-HBe-status, and hepatitis B virus DNA in serum could not be found. Children with a higher percentage of hepatitis B core antigen (HBcAg) expression in their liver tissue presented lower…

Cytotoxicity ImmunologicMaleHBsAgAdolescentmedicine.disease_causePathogenesisAntigenmedicineHumansHepatitis B e AntigensCytotoxicityChildTransaminasesHepatitis ChronicHepatitis B virusHepatitisbusiness.industryInfantHepatitis Bmedicine.diseaseCytotoxicity Tests ImmunologicHepatitis BVirologyHepatitis B Core AntigensHBcAgLiverChild PreschoolPediatrics Perinatology and Child HealthImmunologyDNA ViralFemalebusinessEuropean journal of pediatrics
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H-2(d) mice born to and reared by HBeAg-transgenic mothers do not develop T cell tolerance toward the hepatitis B virus core gene products.

2000

The function of the secretory core gene product (HBeAg) of the human hepatitis B virus (HBV) is unknown. It has been proposed that this protein may be passed from the mother to her offspring at the perinatal stage where it might induce immune tolerance. In a previous study we have shown that the murine placenta presents an efficient barrier for the HBe protein and that H-2(b) mice born to HBeAg-positive transgenic mothers do not develop tolerance of specific cytotoxic T cells. In the present work we demonstrate that transgenic mice expressing high serum levels of HBeAg secrete only small amounts of this protein into their milk and excrete minute amounts of the viral gene product in their ur…

Cytotoxicity ImmunologicMaleHepatitis B virusT cellvirusesT-LymphocytesMothersMice TransgenicBiologymedicine.disease_causeLymphocyte ActivationImmune toleranceMiceImmune systemVirologymedicineImmune ToleranceCytotoxic T cellAnimalsHepatitis B e AntigensHepatitis B AntibodiesHepatitis B virusMice Inbred BALB CH-2 Antigensvirus diseasesT-Lymphocytes Helper-InducerHepatitis Bmedicine.diseaseHepatitis BVirologydigestive system diseasesPeptide Fragmentsmedicine.anatomical_structureMilkHBeAgAnimals NewbornImmunologyFemaleCD8T-Lymphocytes CytotoxicVirology
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Association of hepatitis Be antigen (HBeAg) with the core of the hepatitis B virus (HBcAg).

2008

— Three substances (pronase E, sodium dodecylsulfate (SDS) and guanidine hydrochloride) with different chemical actions partially convert HBcAg to HBeAg. This process retains the integrity of the HBcAg particle, which was not different between HBcAg subpopulations, and does not generate HBcAg or HBeAg sub-units. DNA polymerase activity was destroyed by SDS and guanidine hydrochloride, but not by pronase E. Serum HBeAg could not be converted into HBcAg, suggesting that this might be an irreversible process. The data are consistent with the assumption that HBcAg and HBeAg are coded for by the same gene (C gene of the HBV-DNA).

DNA polymerasePronaseDNA-Directed DNA Polymerasemedicine.disease_causeGuanidinesHepatitis B Antigenschemistry.chemical_compoundAntigenmedicineHumansHepatitis B e AntigensGuanidineGuanidineHepatitisHepatitis B virusHepatologybiologyChemistryvirus diseasesSodium Dodecyl Sulfatemedicine.diseaseHepatitis BVirologyHepatitis B Core Antigensdigestive system diseasesHBcAgHBeAgPronasebiology.proteinLiver
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Interferon-α for HBeAg-positive chronic hepatitis B

2004

HBEAG POSITIVEHepatologybusiness.industrymedicine.medical_treatmentInterferon-alphaAlpha interferonImmunotherapyHepatitis Bmedicine.diseaseAntiviral AgentsVirologyHepatitis B ChronicChronic hepatitisInterferon αImmunologyHumansMedicineHepatitis B e AntigensViral diseasebusinessInterferon alfamedicine.drug
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Translation of hepatitis B virus (HBV) surface proteins from the HBV pregenome and precore RNAs in Semliki Forest virus-driven expression.

2004

Hepatitis B virus (HBV) pregenome RNA (pgRNA) serves as a translation template for the HBV core (HBc) protein and viral polymerase (Pol). HBV precore RNA (pcRNA) directs the synthesis of the precore (preC) protein, a precursor of the hepatitis B e antigen (HBeAg). pgRNA and pcRNA were expressed in the Semliki Forest virus (SFV) expression system. Besides the HBc and preC proteins, there was revealed the synthesis of all three forms of HBV surface (HBs) proteins: long (LHBs), middle (MHBs) and short (SHBs), the start codons of which are located more than 1000 nt downstream of the HBc and preC start codons. Moreover, other HBV templates, such as 3′-truncated pgRNA lacking 3′ direct repeat and…

HBV RNA encapsidation signal epsilonHepatitis B virusvirusesGene ExpressionLeaky scanningDNA-Directed DNA Polymerasemedicine.disease_causeSemliki Forest virusVirus ReplicationCell LineViral Envelope ProteinsVirologymedicineAnimalsHepatitis B e AntigensRNA MessengerCloning MolecularProtein PrecursorsHepatitis B virusHepatitis B Surface Antigensbiologyvirus diseasesRNA virusTemplates Geneticbiology.organism_classificationVirologyMolecular biologyHepatitis B Core AntigensImmunohistochemistrySemliki forest virusdigestive system diseasesGenetic translationHBeAgHepadnaviridaeProtein BiosynthesisRNA ViralThe Journal of general virology
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Mixed cryoglobulinemia type II in chronic hepatitis B associated with HBe-minus HBV mutant: Cellular immune reactions and response to interferon trea…

1994

The case of a young female patient with chronic active hepatitis B, vasculitic purpura, edema, and circulating immune complexes due to mixed Cryoglobulinemia is described. Serum transami-nases were elevated. Serological assays showed hepatitis B surface antigen (HBsAg), antibody to hepatitis B e antigen (anti-HBe), and antibody to hepatitis B core antigen (anti-HBc) antibodies but no antibody to hepatitis C virus (anti-HCV) or antibody to hepatitis delta virus (anti-HDV) antibodies. Using hepatitis B virus-polymerase chain reaction (HBV-PCR) and direct sequencing a precore/core (preC/C) mutant unable to synthesize HBeAg was detected in serum. HBV antigens were demonstrated in the circulatin…

Hepatitis B virusHBsAgAdolescentT-Lymphocytesmedicine.disease_causeAntigenVirologymedicineHumansHepatitis B e AntigensHepatitis ChronicHepatitis B virusImmunity Cellularbiologybusiness.industryInterferon-alphavirus diseasesHepatitis BHepatitis Bmedicine.diseasebiology.organism_classificationVirologydigestive system diseasesHBcAgInfectious DiseasesCryoglobulinemiaHBeAgHepadnaviridaeMutationImmunologyLeukocytes MononuclearFemalebusinessImmune complex diseaseFollow-Up StudiesJournal of Medical Virology
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Ligase Chain Reaction (LCR®) assay for semi-quantitative detection of HBV DNA in mononuclear leukocytes of patients with chronic hepatitis B

1996

Summary. A ligase chain reaction (LCR®)-based approach to detect hepatitis B virus (HBV) DNA in peripheral blood mononuclear cells (PBMC) is described. Using this new amplification technique, we determined semi-quantitatively the amount of a short HBV S-gene fragment in PBMC lysates of 25 patients with different forms of chronic hepatitis (group A (n= 8), hepatitis B s antigen (HBsAg)+/hepatitis B e antigen (HBeAg)+; group B (n= 9), HBsAg+/HBeAg-; group C (n= 8), HBsAg-/HBeAg-). The LCR results were compared with the findings obtained with polymerase chain reaction (PCR) amplification of three distinct HBV gene regions (preS1/2, S and C) and related to the serological profiles of the patien…

Hepatitis B virusHBsAgHepatitis B virus DNA polymerasemedicine.disease_causePolymerase Chain ReactionSensitivity and Specificitylaw.inventionLigaseslawVirologymedicineHumansHepatitis B e AntigensLigase chain reactionPolymerase chain reactionHepatitis B virusHepatitis B Surface AntigensHepatologyClinical Laboratory Techniquesbusiness.industryvirus diseasesHepatitis BHepatitis Bmedicine.diseaseVirologyMolecular biologydigestive system diseasesInfectious DiseasesHBeAgDNA ViralLeukocytes MononuclearPrimer (molecular biology)business
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