Search results for "Histology"

showing 10 items of 985 documents

Lung injury does not aggravate mechanical ventilation-induced early cerebral inflammation or apoptosis in an animal model.

2018

INTRODUCTION:The acute respiratory distress syndrome is not only associated with a high mortality, but also goes along with cognitive impairment in survivors. The cause for this cognitive impairment is still not clear. One possible mechanism could be cerebral inflammation as result of a "lung-brain-crosstalk". Even mechanical ventilation itself can induce cerebral inflammation. We hypothesized, that an acute lung injury aggravates the cerebral inflammation induced by mechanical ventilation itself and leads to neuronal damage. METHODS:After approval of the institutional and state animal care committee 20 pigs were randomized to one of three groups: lung injury by central venous injection of …

MaleARDSCritical Care and Emergency MedicinePulmonologySwinePhysiologymedicine.medical_treatmentVentilator-Induced Lung InjuryInterleukin-1betalcsh:MedicineApoptosisPathology and Laboratory MedicineHippocampusPositive-Pressure RespirationRandom Allocation0302 clinical medicineAnimal CellsImmune PhysiologyMedicine and Health Scienceslcsh:ScienceImmune ResponseAcute Respiratory Distress SyndromeTidal volumeCerebral CortexNeuronsCognitive ImpairmentRespiratory Distress SyndromeInnate Immune SystemMultidisciplinarymedicine.diagnostic_testCognitive NeurologyBrainGeneral MedicineLung InjuryNeurologyAnesthesiaBreathingCytokinesTumor necrosis factor alphamedicine.symptomAnatomyCellular TypesGeneral Agricultural and Biological SciencesResearch ArticleHistologyCognitive NeuroscienceImmunology10208 Institute of NeuropathologyInflammation610 Medicine & healthGenetics and Molecular BiologyGlial Cells1100 General Agricultural and Biological SciencesLung injury03 medical and health sciencesSigns and SymptomsRespiratory Failure1300 General Biochemistry Genetics and Molecular BiologyDiagnostic MedicinemedicineAnimalsMicroglial CellsMechanical ventilationInflammation1000 Multidisciplinarybusiness.industryInterleukin-6Tumor Necrosis Factor-alphalcsh:RBiology and Life Sciences030208 emergency & critical care medicineCell BiologyMolecular Developmentmedicine.diseaseRespiration ArtificialBronchoalveolar lavage030228 respiratory systemImmune SystemCellular NeuroscienceGeneral Biochemistry570 Life sciences; biologyCognitive Sciencelcsh:QbusinessDevelopmental BiologyNeurosciencePloS one
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Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.

2012

Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models, a direct comparison between cell sources has not been performed. The aim of our study was to assess the effect of human multipotent adult progenitor cells (hMAPCs) and human mesenchymal stem cells (hMSCs) on endogenous neurogenesis, angiogenesis and inflammation following stroke. BALB/Ca-RAG 2(-/-) γC(-/-) mice subjected to FeCl(3) thrombosis mediated stroke were intracranially injected with 2 × 10(5) hMAPCs or hMSCs 2 days after stroke and followed for up to 28 days. We could not detect long-term engraftment of either cell population. However, in comparison w…

MaleAnatomy and PhysiologyMousemedicine.medical_treatmentCell therapyMiceCell MovementMolecular Cell BiologyNeurobiology of Disease and RegenerationMedicineMultidisciplinaryNeuronal MorphologyNeurogenesisQRBrainInfarction Middle Cerebral ArteryAnimal ModelsStem-cell therapyStrokeAdult Stem Cellsmedicine.anatomical_structureNeurologyMedicineResearch ArticleAdult stem cellCell PhysiologyHistologyCell SurvivalCerebrovascular DiseasesScienceTherapeutic effectsNeurophysiologyNeovascularization PhysiologicSubventricular zoneMesenchymal Stem Cell TransplantationNeurological SystemModel OrganismsAnimalsHumansProgenitor cellBiologyTransplantationbusiness.industryMultipotent Stem CellsMesenchymal stem cellMultipotent Stem CellCellular NeuroscienceImmunologyCancer researchMolecular NeurosciencebusinesshMAPC and hMSCNeurosciencePLoS ONE
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Mobile microscopy as a screening tool for oral cancer in India: A pilot study

2017

Oral cancer is the most common type of cancer among men in India and other countries in South Asia. Late diagnosis contributes significantly to this mortality, highlighting the need for effective and specific point-of-care diagnostic tools. The same regions with high prevalence of oral cancer have seen extensive growth in mobile phone infrastructure, which enables widespread access to telemedicine services. In this work, we describe the evaluation of an automated tablet-based mobile microscope as an adjunct for telemedicine-based oral cancer screening in India. Brush biopsy, a minimally invasive sampling technique was combined with a simplified staining protocol and a tablet-based mobile mi…

MaleBiopsylcsh:MedicinePilot ProjectsPathology and Laboratory MedicineCytopathologyAutomationUser-Computer Interface0302 clinical medicineImage Processing Computer-AssistedMedicine and Health SciencesMedicineMedical diagnosislcsh:ScienceEarly Detection of CancerMouth neoplasmMicroscopyMultidisciplinaryMiddle AgedOncology030220 oncology & carcinogenesisFemaleMouth NeoplasmsAnatomyTelepathologyResearch ArticleAdultTelemedicinemedicine.medical_specialtyHistologyConcordanceIndiaSurgical and Invasive Medical ProceduresRemote evaluationSensitivity and SpecificityCarcinomasYoung Adult03 medical and health sciencesSigns and SymptomsDiagnostic MedicineCancer Detection and DiagnosisHumansMedical physicsAgedDemographybusiness.industrylcsh:RBiology and Life SciencesCancers and NeoplasmsCell Biology030206 dentistryGold standard (test)Anatomical PathologyMobile phoneLesionslcsh:QCytologybusinessCell PhonePLOS ONE
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Immunohistochemical marker for Na+ CP type Vα (C-20) and heterozygous nonsense SCN5A mutation W822X in a sudden cardiac death induced by mild anaphyl…

2009

A sudden death likely due to mild anaphylactic reaction in a young man is described. Autoptic, histologic, immunohistochemical, and laboratory findings were strongly consistent with the diagnosis of a mild anaphylactic reaction. Genetic molecular analysis, performed on formalin-fixed, paraffin-embedded tissues, showed a mutation described as W822X in a family with electrocardiographic pattern typical of Brugada Syndrome. It results in a nonsense mutation generating a truncated form of the channel protein. The mutation is due to a point substitution of a guanine with an adenine residue (G2466A). Immunohistochemistry and laser scanning confocal microscopy on sections from heart formalin-fixed…

MaleChannellopathies; Confocal laser scanning microscopy; Immunohistochemistry; Na+ CP type Vα (C-20); Sodium channel; Sudden cardiac death; W822X; Adult; Anaphylaxis; Brugada Syndrome; Fatal Outcome; Humans; Male; Muscle Proteins; Myocardium; Myocytes Cardiac; NAV1.5 Voltage-Gated Sodium Channel; Peanut Hypersensitivity; Sodium Channels; Death Sudden Cardiac; Mutation Missense; 2734; Medical Laboratory Technology; HistologyMuscle Proteinsmedicine.disease_causeSodium ChannelsSudden cardiac deathNAV1.5 Voltage-Gated Sodium ChannelNa+ CP type V[alpha] (C-20)Fatal OutcomeMissense mutationMyocytes CardiacConfocal laser scanning microscopyCP type Vα (C-20)Cellular localizationBrugada syndromeBrugada SyndromeMutationChemistrySodium channelChannellopathiesImmunohistochemistryChannellopathies; Confocal laser scanning microscopy; Immunohistochemistry; Na; +; CP type Vα (C-20); Sodium channel; Sudden cardiac death; W822XDeathMedical Laboratory TechnologyCardiologyCardiacAdultmedicine.medical_specialtyHistologyNa+ CP type V[alpha] (C-20) confocal laser scanning microscopy immunohistochemistry sodium channel channellopathies W822X sudden cardiac deathNonsense mutation2734Mutation MissenseSocio-culturaleNa+ CP type Vα (C-20)+Sudden deathPathology and Forensic MedicineInternal medicinemedicineHumansPeanut HypersensitivityNacardiovascular diseasesW822XAnaphylaxisMyocytesSodium channelMyocardiummedicine.diseaseMolecular biologySuddenSudden cardiac deathDeath Sudden CardiacMutationMissenseNa+ CP type V[alpha] (C-20); confocal laser scanning microscopy; immunohistochemistry; sodium channel; channellopathies; W822X; sudden cardiac death
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Fetal rhabdomyomatous nephroblastoma. Pathologic histology and special clinical and biologic features.

1980

Fetal rhabdomyomatous nephroblastoma is a very rare variant of Wilms' tumor. The special clinical and histologic features of this variant are presented on the basis of a case seen in a boy who was 13 months old at nephrectomy 8 months ago and has remained healthy since then. The tumor chiefly consists of fetal striated muscle; it occurs predominantly in infancy and early childhood and is often bilateral. Angiography shows a relative paucity of vessels. The volume of the tumor (determined by ultrasonography) decreases only slightly after preoperative radio- and chemotherapy because of a predominance of mesenchymal structures. The finding of nodular renal blastema in renal parenchyma with an …

MaleChemotherapyPathologymedicine.medical_specialtyFetusmedicine.diagnostic_testbusiness.industrymedicine.medical_treatmentInfantWilms' tumorHistologymedicine.diseaseNephrectomyWilms TumorNephrectomyKidney NeoplasmsFetal rhabdomyomaPediatrics Perinatology and Child HealthAngiographyRhabdomyosarcomamedicineHumansbusinessRhabdomyosarcomaEuropean journal of pediatrics
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Blue-light imaging compared with high-definition white light for real-time histology prediction of colorectal polyps less than 1 centimeter: a prospe…

2019

Blue-light imaging (BLI) is a new chromoendoscopy technique, potentially useful for differentiating neoplastic from nonneoplastic lesions. The present study was aimed at comparing BLI with high-definition white light (HDWL) in the real-time histology prediction of colon polyps 10 mm.Consecutive outpatients undergoing colonoscopy with the ELUXEO 7000 endoscopy platform and 760 series video colonoscopes (Fujifilm Co, Tokyo, Japan) who had at least 1 polyp 10 mm were randomized to BLI or HDWL for polyp characterization. The accuracy of high-confidence real-time histology prediction (adenoma vs not adenoma) by either BLI or HDWL for polyps 10 mm (primary end-point) and diminutive (≤5 mm) polyps…

MaleColonoscopyColonic PolypsSensitivity and SpecificityChromoendoscopy03 medical and health sciencesAdenomatous PolypsNarrow Band Imaging0302 clinical medicineWhite lightMedicineHumansRadiology Nuclear Medicine and imagingProspective randomized studyBlue lightAgedCentimetermedicine.diagnostic_testbusiness.industryGastroenterologyIntestinal PolypsHistologyColonoscopyMiddle Ageddigestive system diseasesEndoscopyTumor Burden030220 oncology & carcinogenesis030211 gastroenterology & hepatologyFemalebusinessNuclear medicineColorectal NeoplasmsGastrointestinal endoscopy
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Intrinsic Expression of Drug Resistance-Associated Factors in Meningiomas

2001

Meningiomas, commonly benign tumors, rarely display aggressive behavior by recurrences and invasion. In addition to surgery, irradiation is beneficial for recurrent, atypical, and malignant meningiomas. The role of chemotherapy, however, remains controversial, although there is evidence that meningiomas respond well to adjuvant chemotherapy. A major obstacle in chemotherapy remains drug resistance with reduced cellular drug accumulation through membrane efflux pumps, drug detoxification, and alterations in drug target specificity. In 84 classic, atypical, and malignant meningiomas, the immunohistochemical expression profile of P-glycoprotein (P-gp), multidrug resistance-associated protein (…

MaleDrugHistologyAdolescentmedia_common.quotation_subjectmedicine.medical_treatmentAntineoplastic AgentsDrug resistancePathology and Forensic MedicineMeningeal Neoplasmsotorhinolaryngologic diseasesmedicineHumansMetallothioneinneoplasmsAgedmedia_commonAged 80 and overChemotherapybiologybusiness.industryTopoisomeraseDrug detoxificationMiddle AgedImmunohistochemistryNeoplasm Proteinsnervous system diseasesMedical Laboratory TechnologyDrug Resistance NeoplasmDisease ProgressionCancer researchbiology.proteinImmunohistochemistryFemaleEffluxMeningiomabusinessApplied Immunohistochemistry & Molecular Morphology
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Evaluation of the clinical and histological effectiveness of isoretinoin in the therapy of oral leukoplakia. Ten years of experience: is management s…

2006

Various studies have evaluated the therapeutic effectiveness of vitamin A derivatives in the treatment of oral leukoplakia (OL). Not all the studies have shown concordant results. The aim of our study, which has a 10-year follow-up, was to test the effectiveness of topical therapy based on 0.18% isotretinoin by comparing it with that most frequently used, i.e., at 0.05% concentration. Forty patients with an established diagnosis of OL were involved in the study. The patients were randomly divided into two groups and the drug was administered topically at 0.05% and 0.18% concentrations. The drug was applied twice a day for 3 consecutive months; then it was suspended for 1 month, and the biop…

MaleDrugVitaminmedicine.medical_specialtyAdministration Topicalmedia_common.quotation_subjectlaw.inventionchemistry.chemical_compoundKeratolytic AgentsRandomized controlled trialRecurrencelawmedicineHumansEstablished diagnosisIsotretinoinIsotretinoinAgedmedia_commonLeukoplakiaDose-Response Relationship Drugbusiness.industryoral leukoplakia isotretinoinHistologyMiddle Agedmedicine.diseaseDermatologySurgeryOral leukoplakiachemistryFemaleLeukoplakia Oralbusinessmedicine.drug
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Morphogenesis of mechanoreceptor and epidermal cells of crickets during the last instar, and its relation to molting-hormone level.

1980

(1) The fine structure of the cercal campaniform sensilla and epidermal cells of Gryllus bimaculatus Deg. (Saltatoria, Gryllidae) was examined, and the ecdysteroid level was monitored throughout the last larval instar. (2) The epidermal cells show changes in shape, cytoplasmic inclusions and differentiation of the apical cell membrane, coupled to the phases of buildup and breakdown of the (cercus) cuticle. (3) The imaginal epicuticle of the epidermal cells begins to form later (by about approximately 6h) than that of the campaniform sensilla. (4) The campaniform sensilla were studied with respect to (a) the morphogenesis of the cuticular apparatus, (b) the inclusion of phenol oxidases in th…

MaleEcdysoneanimal structuresHistologyCuticleCampaniform sensillaApolysisMolting cyclePathology and Forensic Medicinechemistry.chemical_compoundAnimalsEcdysteroidintegumentary systembiologyGryllus bimaculatusfungiMetamorphosis BiologicalCell BiologyAnatomybiology.organism_classificationCell biologychemistryEpidermal CellsEcdysisLarvaOrthopteraFemaleMechanoreceptorsEcdysoneCell and tissue research
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Training labels for hippocampal segmentation based on the EADC-ADNI harmonized hippocampal protocol

2015

Abstract Background The European Alzheimer's Disease Consortium and Alzheimer's Disease Neuroimaging Initiative (ADNI) Harmonized Protocol (HarP) is a Delphi definition of manual hippocampal segmentation from magnetic resonance imaging (MRI) that can be used as the standard of truth to train new tracers, and to validate automated segmentation algorithms. Training requires large and representative data sets of segmented hippocampi. This work aims to produce a set of HarP labels for the proper training and certification of tracers and algorithms. Methods Sixty-eight 1.5 T and 67 3 T volumetric structural ADNI scans from different subjects, balanced by age, medial temporal atrophy, and scanner…

MaleEpidemiologyIntraclass correlationpathology [Cognitive Dysfunction]methods [Pattern Recognition Automated]Hippocampal formationHippocampusFunctional LateralityPattern Recognition Automatedpathology [Alzheimer Disease]ddc:616.89methods [Magnetic Resonance Imaging]methods [Image Processing Computer-Assisted]Image Processing Computer-AssistedSegmentationHARPAged 80 and overmedicine.diagnostic_testHealth PolicyOrgan SizeMiddle AgedMagnetic Resonance Imaginginstrumentation [Magnetic Resonance Imaging]Temporal LobePsychiatry and Mental healthFemalePsychologymethods [Neuroimaging]Algorithmsmethods [Imaging Three-Dimensional]anatomy & histology [Hippocampus]educationNeuroimagingTemporal lobeCellular and Molecular NeuroscienceImaging Three-DimensionalDevelopmental NeuroscienceNeuroimagingAlzheimer DiseasemedicineHumansCognitive Dysfunctionddc:610AgedProtocol (science)business.industryReproducibility of ResultsMagnetic resonance imagingpathology [Temporal Lobe]pathology [Hippocampus]Neurology (clinical)Geriatrics and GerontologyAtrophyNuclear medicinebusinessNeuroscience
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