Search results for "Histology"
showing 10 items of 985 documents
Silybin enhances mitochondrial function and inhibits NFkB activation in murine nonalcoholic fatty liver disease
2010
Italian Journal of Anatomy and Embryology, Vol 115, No 1/2 (Supplement) 2010
Feinstruktur der Cheliceren-Dr�se von Nemastoma dentigerum Canestrini (Opiliones, Nemastomatidae)
1973
1. Nemastoma dentigerum Canestrini besitzt als sekundares mannliches Geschlechtsmerkmal eine Apophyse auf dem ersten Chelicerenglied, die eine Druse enthalt. 2. Die einzelnen Drusenzellen schliesen sich jeweils zu Dreiergruppen zusammen, deren distales Ende immer von 3 Hullzellen umfast wird. Diese 6 Zellen bilden eine funktionelle Einheit innerhalb des komplexen Drusenorgans. Jede Drusenzelle gliedert sich in 3 Abschnitte: 1. in den basalen kernhaltigen Teil, 2. in den mittleren sehr langen Drusenzellschlauch und 3. in den distalen Kopf mit Mikrovilli-Becher. 3. An der Sekretion beteiligen sich 2 Zelltypen: die 3 Drusenzellen und die innere Hullzelle (H1). Das Sekret beider Zelltypen gelan…
Cryptochrome in Sponges: A Key Molecule Linking Photoreception with Phototransduction
2013
Sponges (phylum: Porifera) react to external light or mechanical signals with contractile or metabolic reactions and are devoid of any nervous or muscular system. Furthermore, elements of a photoreception/phototransduction system exist in those animals. Recently, a cryptochrome-based photoreceptor system has been discovered in the demosponge. The assumption that in sponges the siliceous skeleton acts as a substitution for the lack of a nervous system and allows light signals to be transmitted through its glass fiber network is supported by the findings that the first spicules are efficient light waveguides and the second sponges have the enzymatic machinery for the generation of light. Now…
Monoclonal antibodies SMI 311 and SMI 312 as tools to investigate the maturation of nerve cells and axonal patterns in human fetal brain
1998
Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and dendrites or axons. Non-phosphorylated neurofilaments typical of somata and dendrites can be visualized with the aid of monoclonal antibody SMI 311, whereas antibody SMI 312 is directed against highly phosphorylated axonal epitopes of neurofilaments. The maturation of neuronal types, the development of area-specific axonal networks, and the gradients of maturation can thus be …
NG2-expressing cells in the nervous system: role of the proteoglycan in migration and glial–neuron interaction
2005
The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. In the mouse CNS, expression of NG2 protein is already observed at embryonic day 13 and peaks between postnatal days 8 and 12. NG2+ cells persist in grey and white matter in adult mouse brain: cells in the developing and adult brain show clear differences in migration, cell-cycle length and lineage restriction. Several groups have provided evidence that subpopulations of NG2+ cells can generate neurons in vivo. Neuronal stimulation in the developin…
Morphological aspects of the neuronal ceroid lipofuscinoses
2000
Morphological aspects of the neuronal ceroid lipofuscinoses (NCL) encompass two main features: loss of nerve cells and accumulation of autofluorescent lipopigments within cellular compartments. The former requires histology and morphometry for assessment, while the latter necessitates fluorescence microscopy, electron microscopy, and immunohistochemistry. Accumulation of lipopigments is widespread throughout the central nervous system and extracerebrally. The latter feature enables diagnosis of NCL and its clinical subtype. Loss of neurons is most pronounced in cerebral and cerebellar cortices, in early childhood forms. In subcortical grey matter and in later-onset forms, juvenile and adult…
Supravital Uptake of Cationic Dyes by Mast Cell Granules: A Light and Electron Microscope Study
1994
Methylene blue and neutral red were selected for staining mast cell granules by supravital injections. A new technique was applied for embedding in paraffin and Araldite without dislocation or loss of dye. Stabilization and electron microscopic identification of the dyes were achieved by transforming them into electron-dense precipitates using phosphomolybdic acid dissolved in a paraformaldehyde-glutaraldehyde mixture to preserve the ultrastructure of the tissues. It was found that in general the intensity of the light microscopic staining correlated directly with the electron density. Closer study revealed that not all cytoplasmic granules exhibited the same strong affinity for the cationi…
Metabolism of reduced pyridine nucleotides in ascites cell nuclei
1964
1. The conditions are described under which the fluorescence due to reduced pyridine nucleotides can be studied separately at nuclear and cytoplasmic sites of glass-grown ascites cells, by the use of a flow chamber in the microfluorimeter ofChance andLegallais.
The “Journal of Functional Morphology and Kinesiology” Journal Club Series: Highlights of Recent Papers in Pediatric Exercise
2019
We are glad to introduce the ninth Journal Club. This edition is focused on several relevant studies published in the last years in the field of Pediatric Exercise, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share with you the passion for the sport as seen also from the scientific point of view. The Editorial Board members wish you an inspiring lecture.
Optimization of a decellularized protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study
2021
[EN] Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from …