Search results for "Hot Temperature"

showing 10 items of 310 documents

Cortical Temperature Change: A Tool for Modulating Brain States?12

2016

Reduction in temperature depolarizes neurons by a partial closure of potassium channels but decreases the vesicle release probability within synapses. Compared with cooling, neuromodulators produce qualitatively similar effects on intrinsic neuronal properties and synapses in the cortex. We used this similarity of neuronal action in ketamine-xylazine-anesthetized mice and non-anesthetized mice to manipulate the thalamocortical activity. We recorded cortical electroencephalogram/local field potential (LFP) activity and intracellular activities from the somatosensory thalamus in control conditions, during cortical cooling and on rewarming. In the deeply anesthetized mice, moderate cortical co…

Cerebral CortexMalewaking stateHot TemperatureAction PotentialsNeuronal ExcitabilityElectroencephalographyBrain WavesCold TemperatureMice Inbred C57BLMicebrain statesThalamusBiological ClocksneuromodulationNeural PathwaysCommentaryepilepsyAnimalsFemalesleepslow-wave rhythmseNeuro
researchProduct

Stress response in mesoangioblast stem cells

2006

Stem cells are presumed to survive various stresses, since they are recruited to areas of tissue damage and regeneration, where inflammatory cytokines and cytotoxic cells may result in severe cell injury. We explored the ability of mesoangioblasts to respond to different cell stresses such as heat, heavy metals and osmotic stress, by analyzing heat shock protein (HSP)70 synthesis as a stress indicator. We found that the A6 mesoangioblast stem cells constitutively synthesize HSP70 in a heat shock transcription factor (HSF)-independent way. However, A6 respond to heat shock and cadmium treatment by synthesizing HSP70 over the constitutive expression and this synthesis is HSF1 dependent. The e…

Chloramphenicol O-AcetyltransferaseHot TemperatureOsmotic shockRecombinant Fusion ProteinsBlotting WesternHypertonic SolutionsElectrophoretic Mobility Shift AssayBiologyResponse ElementsTransfectionMesodermMiceSTRESS RESPONSE STEM CELLS MOUSE MESOANGIOBLASTS.Heat Shock Transcription FactorsHeat shock proteinMetals HeavyAnimalsRNA MessengerHSF1Promoter Regions GeneticMolecular BiologyCells CulturedMesoangioblastHSC70 Heat-Shock ProteinsCell BiologyTransfectionHematopoietic Stem CellsMolecular biologyCell biologyHsp70Heat shock factorDNA-Binding ProteinsGene Expression RegulationStem cellTranscription Factors
researchProduct

Water-Soluble Chlorophyll Protein (WSCP) Stably Binds Two or Four Chlorophylls

2017

Water-soluble chlorophyll proteins (WSCPs) of class IIa from Brassicaceae form tetrameric complexes containing one chlorophyll (Chl) per apoprotein but no carotenoids. The complexes are remarkably stable toward dissociation and protein denaturation even at 100 °C and extreme pH values, and the Chls are partially protected against photooxidation. There are several hypotheses that explain the biological role of WSCPs, one of them proposing that they function as a scavenger of Chls set free upon plant senescence or pathogen attack. The biochemical properties of WSCP described in this paper are consistent with the protein acting as an efficient and flexible Chl scavenger. At limiting Chl concen…

ChlorophyllModels Molecular0106 biological sciences0301 basic medicineProtein DenaturationHot TemperatureLightLight-Harvesting Protein ComplexesGene ExpressionThylakoids01 natural sciencesBiochemistryProtein Structure SecondaryDissociation (chemistry)law.inventionchemistry.chemical_compoundlawpolycyclic compoundsDenaturation (biochemistry)CarotenoidPlant Proteinschemistry.chemical_classificationSinglet OxygenProtein Stabilityfood and beveragesHydrogen-Ion ConcentrationBiochemistryRecombinant DNAOxidation-ReductionProtein BindingRecombinant Fusion ProteinsBrassicamacromolecular substancesBiology03 medical and health sciencesProtein DomainsTetramerPlant senescenceChlorophyll APeasWaterOxygen030104 developmental biologyWater solubleSolubilitychemistryChlorophyllProtein MultimerizationApoproteins010606 plant biology & botanyBiochemistry
researchProduct

Analysis of eicosapentaenoic and docosahexaenoic acid geometrical isomers formed during fish oil deodorization.

2006

International audience; Addition of long-chain polyunsaturated fatty acids (LC-PUFAs) from marine oil into food products implies preliminary refining procedures of the oil which thermal process affects the integrity of LC-PUFAs. Deodorization, the major step involving high temperatures, is a common process used for the refining of edible fats and oils. The present study evaluates the effect of deodorization temperature on the formation of LC-PUFA geometrical isomers. Chemically isomerized eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were used as reference samples. Fish oil samples have been deodorized at 180, 220 and 250 °C for 3 h and pure EPA and DHA fatty acid methyl esters…

Chromatography GasHot TemperatureDocosahexaenoic Acids[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering030309 nutrition & dietetics01 natural sciencesBiochemistryHigh-performance liquid chromatographyAnalytical ChemistrySILVER-ION CHROMATOGRAPHY03 medical and health sciencesFish OilsIsomerismDECOSAHEXAENOIC ACID[SDV.IDA]Life Sciences [q-bio]/Food engineeringOrganic chemistry[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringChromatography High Pressure Liquidchemistry.chemical_classificationFlame IonizationFISH OIL0303 health sciencesChromatographyChemistry010401 analytical chemistryOrganic ChemistryFatty acidReproducibility of ResultsGeneral Medicine[SDV.IDA] Life Sciences [q-bio]/Food engineeringFish oilEicosapentaenoic acidTRANS FATTY ACIDS0104 chemical sciencesEicosapentaenoic Acid13. Climate actionDocosahexaenoic acidDEODORIZATIONOdorantsFatty Acids Unsaturatedlipids (amino acids peptides and proteins)Gas chromatographyChromatography Thin LayerIsomerizationCis–trans isomerismTolueneJournal of chromatography. A
researchProduct

High-performance liquid chromatographic determination of Maillard compounds in store-brand and name-brand ultra-high-temperature-treated cows' milk.

2000

Furosine and furfural products of the Maillard reaction are used as specific indicators of the effect of heating treatments on milk quality. Their contents were measured in representative samples of store- and name-brand ultra-high-temperature-treated milks using RP-HPLC with UV detection. Furosine contents ranged from 40.32 to 50.67 and from 65.48 to 310.58 mg/100 g protein in name- and store-brand milks, respectively. Of the furfurals, only hydroxymethylfurfural was detected. The free hydroxymethylfurfural contents of store-brand milks ranged from 0.22 to 1.70 mg/100 g protein. Total hydroxymethylfurfural contents ranged from 0.29 to 0.41 and from 0.72 to 2.21 mg/100 g protein, for name- …

ChromatographyHot TemperatureOrganic ChemistryFood composition dataGeneral MedicineReversed-phase chromatographyFurfuralBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryMaillard Reactionchemistry.chemical_compoundMaillard reactionsymbols.namesakeMilkchemistrysymbolsAnimalsCattleSpectrophotometry UltravioletUv detectionQuantitative analysis (chemistry)HydroxymethylfurfuralChromatography High Pressure LiquidJournal of chromatography. A
researchProduct

Filter-based infrared detectors for high temperature size exclusion chromatography analysis of polyolefins: calibration with a small number of standa…

2012

Infrared detection has been shown to be very appropriate for high temperature analysis of polyolefins. After some early reports in which dispersive or single-band filter-based detectors were applied, Fourier transform detectors have been described for this application, in order to improve the method sensitivity. Modern simple filter-based detectors prove, however, comparable sensitivity while providing a number of practical advantages when coupled to chromatographic systems: reduced cell volume, simplified hardware, continuous generation of absorbance chromatograms, as well as simpler data collection and processing. A practical method for calibration, using multiple-band signals obtained wi…

ChromatographyHot TemperatureResolution (mass spectrometry)Spectrophotometry InfraredChemistryOrganic ChemistryDetectorGeneral MedicinePolyenesSignal-To-Noise RatioBiochemistryAnalytical ChemistryAbsorbanceMolecular Weightsymbols.namesakeSignal-to-noise ratioFourier transformFilter (video)CalibrationMultivariate AnalysisCalibrationsymbolsChromatography GelLinear ModelsSensitivity (control systems)Journal of chromatography. A
researchProduct

Cr(VI)/Cr(III) and As(V)/As(III) Ratio Assessments in Jordanian Spent Oil Shale Produced by Aerobic Combustion and Anaerobic Pyrolysis

2011

With the increase in the awareness of the public in the environmental impact of oil shale utilization, it is of interest to reveal the mobility of potentially toxic trace elements in spent oil shale. Therefore, the Cr and As oxidation state in a representative Jordanian oil shale sample from the El-Lajjoun area were investigated upon different lab-scale furnace treatments. The anaerobic pyrolysis was performed in a retort flushed by nitrogen gas at temperatures in between 600 and 800 °C (pyrolytic oil shale, POS). The aerobic combustion was simply performed in porcelain cups heated in a muffle furnace for 4 h at temperatures in between 700 and 1000 °C (burned oil shale, BOS). The high loss-…

ChromiumHot TemperatureJordanChemistryMetallurgychemistry.chemical_elementGeneral ChemistryRetortCombustionArseniclaw.inventionChromiumchemistry.chemical_compoundPetroleumCalcium carbonatelawEnvironmental chemistryEnvironmental ChemistryMuffle furnaceOxidation-ReductionPyrolysisOil shaleArsenicEnvironmental Science & Technology
researchProduct

Inactivation and structural changes of polyphenol oxidase in quince ( Cydonia oblonga Miller) juice subjected to ultrasonic treatment

2020

Background Polyphenol oxidase (PPO) is considered a problem in the food industry because it starts browning reactions during fruit and vegetable processing. Ultrasonic treatment is a technology used to inactivate the enzyme; however, the mechanism behind PPO inactivation is still unclear. For this reason, the inactivation, aggregation, and structural changes in PPO from quince juice subjected to ultrasonic treatments were investigated. Different intensities and times of ultrasonic treatment were used. Changes in the activity, aggregation, conformation, and structure of PPO were investigated through different structural analyses. Results Compared to untreated juice, the PPO activity in treat…

Circular dichroismHot TemperatureChemical PhenomenaFood Handling030309 nutrition & dieteticsColorProtein aggregationPolyphenol oxidaseProtein Structure Secondary03 medical and health sciences0404 agricultural biotechnologyVegetablesBrowningUltrasonicsParticle SizeRosaceaeProtein secondary structurePlant Proteinschemistry.chemical_classification0303 health sciencesNutrition and Dieteticsbusiness.industryChemistryCircular DichroismUltrasound04 agricultural and veterinary sciencesHydrogen-Ion Concentration040401 food scienceProtein tertiary structureMaillard ReactionFruit and Vegetable JuicesEnzymeFruitBiophysicsbusinessAgronomy and Crop ScienceCatechol OxidaseFood ScienceBiotechnologyJournal of the Science of Food and Agriculture
researchProduct

Nuclear Spin Relaxation in Viscous Liquids: Relaxation Stretching of Single-Particle Probes

2021

Spin-lattice relaxation rates R1(ω,T), probed via high-field and field-cycling nuclear magnetic resonance (NMR), are used to test the validity of frequency-temperature superposition (FTS) for the reorientation dynamics in viscous liquids. For several liquids, FTS is found to apply so that master curves can be generated. The susceptibility spectra are highly similar to those obtained from depolarized light scattering (DLS) and reveal an excess wing. Where FTS works, two approaches are suggested to access the susceptibility: (i) a plot of deuteron R1(T) vs the spin-spin relaxation rate R2(T) and (ii) a plot of R1(T) vs an independently measured reference time τref(T). Using single-frequency s…

Coupling constantCouplingHot TemperatureMagnetic Resonance SpectroscopyMaterials scienceViscosityTemperatureViscous liquidMagnetic Resonance ImagingMolecular physicsLight scatteringSpectral lineSurfaces Coatings and FilmsSuperposition principleDeuteriumMaterials ChemistryRelaxation (physics)Physical and Theoretical ChemistryThe Journal of Physical Chemistry B
researchProduct

Biodiversity and dairy traits of lactic acid bacteria from foliage of aromatic plants before and after dehydration process monitored by a smart senso…

2020

ABSTRACT The main hypothesis of this work was to evaluate the presence of lactic acid bacteria (LAB) intrinsically resistant to plant essential oils in sage (Salvia officinalis L.) and laurel (Laurus nobilis), for future applications in functional cheese production by addition of aromatic herbs. The effect of the drying process on the viability of LAB was evaluated with three biomass densities (3, 4 and 5 kg/m2). The drying densities did not affect weight loss, but influenced the levels of LAB of sage and laurel. A total of 10 different strains of Enterococcus faecium, Enterococcus mundtii, Enterococcus raffinosus and Leuconostoc mesenteroides were identified from laurel, while sage did not…

DNA BacterialHot TemperatureEnterococcus mundtiived/biology.organism_classification_rank.speciesMicrobial Sensitivity TestsMicrobiologyLauraceaefoodLaurus nobilisCheeseLactobacillalesRNA Ribosomal 16Ssmart systemGeneticsdryingFood scienceDesiccationSalvia officinalisMolecular BiologyPhylogenyMicrobial Viabilitybiologyved/biologyChemistryEnterococcus raffinosusSAGESalvia officinalisSettore AGR/09 - Meccanica Agrariafood and beveragesBiodiversitybiology.organism_classificationfood.foodAnti-Bacterial AgentsRandom Amplified Polymorphic DNA Techniquelactic acid bacterialaurelsageEnterococcusLeuconostoc mesenteroidesFood Microbiologydairy potentialSettore AGR/16 - Microbiologia AgrariaEnterococcus faeciumFEMS Microbiology Letters
researchProduct