Search results for "Hsp27"

showing 3 items of 43 documents

Inhibition of HSP27 blocks fibrosis development and EMT features by promoting Snail degradation

2013

Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by myofibroblast proliferation. Transition of epithelial/mesothelial cells into myofibroblasts [epithelial-to-mesenchymal transition (EMT)] occurs under the influence of transforming growth factor (TGF)-β1, with Snail being a major transcription factor. We study here the role of the heat-shock protein HSP27 in fibrogenesis and EMT. In vitro, we have up- and down-modulated HSP27 expression in mesothelial and epithelial cell lines and studied the expression of different EMT markers induced by TGF-β1. In vivo, we inhibited HSP27 with the antisense oligonucleotide OGX-427 (in phase II clinical trials as anticancer agent)…

endocrine systemPathologymedicine.medical_specialtyEpithelial-Mesenchymal Transitionanimal structuresSnailsHSP27 Heat-Shock ProteinsBiologyBiochemistryCell LineRats Sprague-DawleyTransforming Growth Factor beta103 medical and health sciencesIdiopathic pulmonary fibrosis0302 clinical medicineIn vivoFibrosisPulmonary fibrosisGeneticsmedicineAnimalsHumansEpithelial–mesenchymal transitionMolecular Biology030304 developmental biology0303 health sciencesGene knockdownEpithelial CellsOligonucleotides AntisenseThionucleotidesCadherinsmedicine.diseaseFibrosisRats3. Good health030220 oncology & carcinogenesisembryonic structuresCancer researchMyofibroblastTranscription FactorsBiotechnologyTransforming growth factorThe FASEB Journal
researchProduct

SFRR-E Young Investigator AwardeeαB-crystallin modulation after acute exercise in skeletal muscle: the role of oxidative stress and fiber composition.

2015

αB-crystallin (CRYAB) is a member of the small heat shock proteins implicated in various biological functions, particularly in skeletal muscle where it is involved in adaptive remodelling processes, activation of gene transcription and stabilization of nascent proteins.In this research we analysed αB-crystallin' response in mouse gastrocnemius at 15' and 30' of recovery from an acute aerobic exercise (1hour), correlating its modulation with oxidative stress level and fiber composition, red (RG) and white gastrocnemius (WG).We found for the first time that the acute exercise lead to a short term, specific increase of phospho-αB-crystallin level (pCRYAB) in the RG, while no changes were obser…

medicine.medical_specialtybiologySkeletal muscleOxidative phosphorylationmedicine.disease_causeBiochemistryHsp70medicine.anatomical_structureEndocrinologyHsp27BiochemistryCrystallinPhysiology (medical)Internal medicinemedicinebiology.proteinPhosphorylationAerobic exerciseOxidative stressFree radical biologymedicine
researchProduct

Prognostic value of HSP27 in head and neck squamous cell carcinoma: a retrospective analysis of 57 tumours.

2006

Background: The aims of the present study were to assess the prevalence of HSP27 expression in oral squamous cell carcinoma (OSCC) and to verify whether HSP27 can be considered to be a marker of prognosis in patients with OSCC. Materials and Methods: The immunohistochemical expression of HSP27 was evaluated in 57 OSCC, who received standard treatment and monitoring. After grouping for HSP27 expression, OSCCs were statistically analysed for the variables age, gender, histological grading, TNM, staging and survival rate. Univariate and multivariate analyses were performed. Results: HSP27 was found to be reduced in 31 OSCC and was normally expressed in 26 OSCC. The pattern and intensity of HSP…

squamous cell carcinomaAdultAged 80 and overMaleAdolescentHSP27Middle AgedPrognosissurvivalImmunohistochemistrySurvival RateLogistic ModelsHead and Neck NeoplasmsCarcinoma Squamous CellHumanshead and neck cancerFemaleHeat-Shock ProteinsAgedNeoplasm StagingRetrospective StudiesAnticancer research
researchProduct