Search results for "Hsp"
showing 10 items of 557 documents
Mouse mesoangioblasts release Hsp70 in a controlled manner through membrane vesicle shedding
2009
Mesoangioblaststs (Mabs) are mesodermal stem cells associated with vessels which can differentiate into different mesoderm cell types. They have the property to cross endothelial barrier and when injected into circulation they localize into damaged tissues. A6 cells, a clone of mouse Mabs, express Hsp70 protein in physiological conditions and this synthesis may be required to answer to several intra- and/or extra-cellular needs. It was found that Hsp70 may be released outside from several type of cells, but its role remains still undefined. In order to clarify the reason of Hsp70 constitutive expression, we prepared several A6 clones with different levels of Hsp70 expression by stable RNA i…
Effect of sodium dodecyl sulfate (SDS) on stress response in the Mediterranean mussel (Mytilus Galloprovincialis): Regulatory volumedecrease (Rvd) an…
2014
In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are assessed on the Mediterranean mussel (. Mytilus galloprovincialis), exposed for 18 days at a concentration ranging from 0.1. mg/l to 1. mg/l. The effects are monitored using biomarkers related to stress response, such as regulatory volume decrease (RVD), and to oxidative stress, such as reactive oxygen species (ROS), endogenous antioxidant systems and Hsp70 levels. The results demonstrate that cells from the digestive gland of M. galloprovincialis, exposed to SDS were not able to perform the RVD owing to osmotic stress. Further, SDS causes oxidative stress in treated organisms, as demonstrated by the incre…
THE ROLE OF HSP60 IN AMYLOID BETA PATHWAY: RELEVANCE TO ALZHEIMER’S DISEASE
Alzheimer’s disease (AD) is a devastating neurodegenerative disorder affecting more than 40 million individuals worldwide. The high number of factors triggering the onset of AD justifies the current absence of disease-modifying therapies. The involved pathological mechanisms are still elusive and, therefore, the finding of effective therapies requires further elucidation of biomolecular mechanisms controlling AD pathogenesis. Particularly, the aberrant amyloidogenic cleavage of amyloid precursor protein (APP), amyloid beta (Aβ) peptide misfolding and oligomerization, and the impairment of the protein quality control machinery are key hallmarks characterizing the onset of the disease. Furthe…
The pathogenetic role of the chaperonin Hsp60 in chronic obstructive pulmonary disease: new data and perspectives
2012
The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90
2022
Breast cancer (BC) is a major public health problem, with key pieces of information needed for developing preventive and curative measures still missing. For example, the participation of the chaperone system (CS) in carcinogenesis and anti-cancer responses is poorly understood, although it can be predicted to be a crucial factor in these mechanisms. The chief components of the CS are the molecular chaperones, and here we discuss four of them, Hsp27, Hsp60, Hsp70, and Hsp90, focusing on their pro-carcinogenic roles in BC and potential for developing anti-BC therapies. These chaperones can be targets of negative chaperonotherapy, namely the elimination/blocking/inhibition of the chaperone(s)…
Hsp60 and AChR cross-reactivity in myasthenia gravis: An update.
2010
Focusing on a quantification and characterization of extracellular vesicles from blood in brain tumors
Molecular chaperones are required to maintain the proteome in a folded and functional state. Chaperones are structurally and functionally normal but participate in pathways that favor disease, as a tumorigenesis (1). The incidence of brain tumors is increasing rapidly and surgery is the first therapeutic intervention to safeguard the patient's life. The prognosis is poor even after surgical resection, followed by post-operatory chemo- and radio-therapy (2). Tumor-secreted extracellular vesicles (EVs) are critical mediators of intercellular communication between tumor cells and stromal cells in local and distant microenvironments. EVs play an essential role in both primary tumor growth and m…
Two isoelectric variants of HSP10 are down-regulated by cigarette smoke exposure in airway cells: A Proteomic study
2008
NOVEL ANTAGONISTS FOR AN Hsp60-BASED ANTICANCER CHAPERONOTHERAPY
2012
The Heat shock proteins (Hsps) are nowadays considered the most important cell chaperones, which result overexpressed in response to a number of cell stress stimuli.In tumor cells, when Hsp60 accumulates in the cytosol, without mitochondrial release, it exerts an anti-apoptotic effect, by inhibiting pro-caspase-3 (pC3) activation. In this context, our study aims to elucidate the structural details of the interaction between Hsp60 and pC3 and to design novel antagonists able to specifically block this interaction. The analysis of virtual screening results highlights the 4-(3-chloro-4-fluorophenylamino)- 6-[(1H-imidazol-4-yl-methyl)-3-carbonitrile-quinolines of type 1 and the N-{5-[1H-imidazo…