Search results for "Human immunodeficiency"

Control of HIV-infection with Visits Scheduled Every Four or Every Six Months. A Comparative Randomized Study

2011

IL-23: a master regulator in Crohn disease

2007

Three studies should shift thinking about the causes of inflammatory bowel disease. It seems that researchers have been focusing on the wrong cytokine as a driving force.

Evidence for a direct interaction of Rev protein with nuclear envelop mRNA-translocation system.

1991

The interaction of the Rev protein from human immunodeficiency virus type 1 (HIV-1) with the nucleocytoplasmic mRNA-transport system was investigated. In gel-shift assay, the recombinant Rev protein used in this study selectively bound to the Rev-responsive element (RRE) region of HIV-1 env-specific RNA. Nitrocellulose-filter-binding studies and Northern/Western-blotting experiments revealed an association constant of approximately 1 x 10(10) M-1. The Rev protein also strongly bound to isolated nuclear envelopes from H9 cells, containing the poly(A)-binding site (= mRNA carrier) and the nucleoside triphosphatase (= NTPase), which are thought to be involved in nuclear export of poly(A)-rich …

Post-cesarean section morbidity in HIV-positive women

1999

Background The present work is an audit of post-cesarean section morbidity in HIV-positive women in the tertiary teaching hospital La Fe, Valencia, Spain. Study design. Retrospective case-control study. Subjects. Forty-five HIV-positive pregnant women and 90 appropriately matched controls, delivered by cesarean section in the same hospital and managed using a uniform protocol. Main outcome measures. The duration of stay in hospital after cesarean section, the need for postoperative antibiotics and the incidence of major and minor puerperal complications. Baseline characteristics of HIV-positive women were also analyzed in relation to the morbidity after surgery. Statistical analysis. Chi-sq…

Vaccination with TAT-Antigen Fusion Protein Induces Protective, CD8+ T Cell-Mediated Immunity Against Leishmania Major

2010

In murine leishmaniasis, healing is mediated by IFN-γ-producing CD4 + and CD8 + T cells. Thus, an efficacious vaccine should induce Th1 and Tc1 cells. Dendritic cells (DCs) pulsed with exogenous proteins primarily induce strong CD4-dependent immunity; induction of CD8 responses has proven to be difficult. We evaluated the immunogenicity of fusion proteins comprising the protein transduction domain of HIV-1 TAT and the Leishmania antigen LACK ( Leishmania homolog of receptors for activated C kinase), as TAT-fusion proteins facilitate major histocompatibility complex class I-dependent antigen presentation. In vitro , TAT–LACK-pulsed DCs induced stronger proliferation of Leishmania -specific C…

Evolución de la declaración de la tuberculosis en un área sanitaria de la Comunidad Valenciana desde 1987 hasta 1999

2001

Objetivos La infradeclaracion de la tuberculosis (TBC) es un hecho frecuente a escala mundial que ha sido escasa-mente estudiado en nuestro pais. El objetivo de nuestro es-tudio fue conocer el estado de la declaracion de los casos de TBC en nuestra area sanitaria Material y metodo Desde 1987 a 1999 se estudio de for-ma directa la incidencia y el estado de declaracion de los casos de TBC en nuestra area (censo: 106.632 habitantes). Los casos se obtuvieron desde el registro de EDO, anatomia patologica y bacteriologia, con posterior revision de la historia clinica Resultados Se diagnosticaron 410 casos de TBC, de los cuales 378 eran recientes del area (incidencia media anual: 27 por 100.000 ha…

Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineage

2021

The accessory protein Nef of human and simian immunodeficiency viruses (HIV and SIV) is an important pathogenicity factor known to interact with cellular protein kinases and other signaling proteins. A canonical SH3 domain binding motif in Nef is required for most of these interactions. For example, HIV-1 Nef activates the tyrosine kinase Hck by tightly binding to its SH3 domain. An archetypal contact between a negatively charged SH3 residue and a highly conserved arginine in Nef (Arg77) plays a key role here. Combining structural analyses with functional assays, we here show that Nef proteins have also developed a distinct structural strategy—termed the "R-clamp”—that favors the formation …

Rev protein suppression of complex formation between nuclear proteins and rev-responsive element-containing RNA of human immunodeficiency virus-1

1995

The Rev protein from human immunodeficiency virus type 1 (HIV-1) is known to bind Rev responsive element (RRE) sequence of HIV-1 mRNA. This interaction is thought to enhance expression of viral structural proteins but the mechanism for this effect is uncertain. The aim of this study was to investigate (i) whether other cellular proteins also bind to the RRE sequence and (ii) whether binding of cellular proteins to RRE RNA is influenced by Rev protein. Our results revealed that a variety of RNA-protein complexes are formed when in vitro transcribed RRE-containing RNA is incubated with proteins present in HeLa nuclear extracts. The molecular masses of the most prominent bands in RNase protect…

La diagnostica delle malattie infettive: Tra metafora del passato e itinerario nel futuro

1993

A global emergency is threatening mankind, awakening dead ghosts and causing alarm about our present and future: it is the apparently irreversible spread of infectious diseases.This critical and worrying situation should be acknowledged and carefully followed by the radiological profession since all of us – teachers, specialists, professionals – have to deal with imaging diagnostic modalities deriving information from a deceitful and camouflageing anatomo-functional substratum.The increasing spread of infectious diseases is due to a wide range of concomitant factors: HIV infection, drug addiction, migration fenomena and the related opportunistic infections combined with poor hygienic, physi…

How can hepatitis C be prevented in the long term?

2007

Abstract Significant advances have been made in preventing HIV infection among injectors but we still know little about preventing hepatitis C (HCV). Both prevalence and incidence of hepatitis C can remain high among IDUs even in the context of widespread implementation of harm reduction programmes. We need to develop new ways to fill the knowledge gap regarding HCV prevention. One way is to learn from the experts—those IDUs who, after long-term injection in social milieus of high hepatitis C prevalence, nonetheless remain uninfected. We describe a recently commenced program of research that focuses on understanding the strategies, behaviours, and environmental factors associated with "stay…