Search results for "Hybridisation"
showing 6 items of 16 documents
DNA sequence baits targeting ultra-conserved elements in marine invertebrates
2022
This dataset includes four files of DNA sequence baits targeted to ultra-conserved elements (UCEs) identified from four different taxonomic groups of marine invertebrates: Annelida, Bivalvia, Gastropoda and Crustacea. UCE loci are genomic regions of high conservation that can be found throughout the genomes that can be extracted from the genome and sequenced for use in phylogenetic, phylogeographic and population genetic analyses. The UCE loci were located by aligning a minimum of five species’ genomes to a base genome in each taxonomic group and searching for overlapping highly conserved areas. The UCE baits were designed with screening coverage of 4X using the pipeline phyluce v. 1.6.6 (h…
Identification of genes activated during arbuscular mycorrhiza interactions by suppressive substractive hybridisation and cloning of differential exp…
2000
International audience
The monophyly and evolution of Cynara L. (Asteraceae) sensu lato: evidence from the Internal Transcribed Spacer region of nrDNA
2005
The monophyly and evolution of Cynara was investigated using ITS sequence data. Parsimony analysis supports the monophyly of Cynara sensu lato, i.e. including the distinctive taxa C. humilis and C. tournefortii. This contradicts the recent decision to create a new monotypic genus Arcyna for C. tournefortii. A hypothesised close relationship between C. tournefortii and Silybum Adans. is also refuted. Four of the five species of Cynara, for which multiple accessions were sequenced, were shown to be monophyletic but C. baetica was found to be non-monophyletic. Free energy estimates for ITS1 secondary structure and conservation of the 5.8S region suggest that this is not due to the occurrence o…
Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review.
2021
The discovery of telomeric repeats in interstitial regions of plant chromosomes (ITRs) through molecular cytogenetic techniques was achieved several decades ago. However, the information is scattered and has not been critically evaluated from an evolutionary perspective. Based on the analysis of currently available data, it is shown that ITRs are widespread in major evolutionary lineages sampled. However, their presence has been detected in only 45.6% of the analysed families, 26.7% of the sampled genera, and in 23.8% of the studied species. The number of ITR sites greatly varies among congeneric species and higher taxonomic units, and range from one to 72 signals. ITR signals mostly occurs…
From Mobile Crimes to Crimes of Mobility
2020
Piipponen, Mäntymäki and Rodi-Risberg suggest that many contemporary crime narratives across the globe host a heightened interest in diverse and ambiguous mobilities, border crossings and borderlands. They propose that such mobilities and crossings reflect on recent sociocultural developments on local and global levels and communicate specific geopolitical anxieties. They position their own mobilities research perspective within existing crime fiction scholarship, especially within the so-called transnational and spatial turns. Introducing some key observations of mobilities research, they suggest that mobility can be considered both as an object of study in its own right and a critical len…
Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile
2004
Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments…