Search results for "Hybridization"
showing 10 items of 812 documents
Territorial localization of heat shock mRNA production in sea urchin gastrulae.
1985
In situ hybridization experiments with a labeled DNA probe indicate that the ability to respond to heat shock with the production of the mRNA for the 70 kd heat shock protein is segregated into the ectodermal cells already at the gastrula stage or earlier during the embryonic development of Paracentrotus lividus.
Identification and quantification of microbial populations in activated sludge and anaerobic digestion processes
2014
Eight different phenotypes were studied in an activated sludge process (AeR) and anaerobic digester AnD) in a full-scale wastewater treatment plant by means of fluorescent in situ hybridization (FISH) and automated FISH quantification software. The phenotypes were ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosphate-accumulating organisms (PAO), glycogen-accumulating organisms (GAO), sulphate-reducing bacteria (SRB), methanotrophic bacteria and methanogenic archaea. Some findings were unexpected: (a) Presence of PAO, GAO and denitrifiers in the AeR possibly due to unexpected environmental conditions caused by oxygen deficiencies or its ability to survive …
The pattern of neuroblast formation, mitotic domains and proneural gene expression during early brain development in Drosophila.
2003
In the Drosophila embryo, studies on CNS development have so far mainly focused on the relatively simply structured ventral nerve cord. In the trunk, proneural genes become expressed in small cell clusters at specific positions of the ventral neuroectoderm. A lateral inhibition process mediated by the neurogenic genes ensures that only one cell within each proneural cluster delaminates as a neural stem cell (neuroblast). Thus, a fixed number of neuroblasts is formed, according to a stereotypical spatiotemporal and segmentally repeated pattern, each subsequently generating a specific cell lineage. Owing to higher complexity and hidden segmental organisation, the mechanisms underlying the dev…
Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Dro…
2013
In Drosophila, the brain arises from about 100 neural stem cells (called neuroblasts) per hemisphere which originate from the neuroectoderm. Products of developmental control genes are expressed in spatially restricted domains in the neuroectoderm and provide positional cues that determine the formation and identity of neuroblasts. Here, we present a protocol for nonfluorescent double in situ hybridization combined with antibody staining which allows the simultaneous representation of gene expression patterns in Drosophila embryos in up to three different colors. Such visible multiple stainings are especially useful to analyze the expression and regulatory interactions of developmental cont…
Expression Therapies Through Installation
2017
La instalación como vía de expresión híbrida en la que se contempla la utilización del espacio con un sentido de intervención creativa, - arquitectura, pintura, escultura, fotografía, vídeo, performance, danza, dramatización y música- entre otras disciplinas implicadas, proporciona al campo de la terapia un universo sensorial y proposicional que puede tomarse como base conceptual en el proceso terapéutico mediante las artes.Partiendo del análisis sobre aspectos conceptuales y artistas de referencia de la instalación y tomando en consideración aspectos pertenecientes a terapias de expresión artística, se plantean sesiones arteterapéuticas fundamentadas en el uso de la instalación. The hybrid…
FISH mapping of 18S rDNA and (TTAGGG)n sequences in two pipefish species (Gasteroisteiformes: Syngnathidae).
2006
1Istituto di Scienze Marine, Sezione di Venezia, CNR, Castello 1364/a, 30122 Venezia, Italy 2Dipartimento di Biologia Animale, Universita di Palermo, Via Archirafi 18, 90123 Palermo, Italy 3Dipartimento di Scienze Ambientali, Universita “Ca’ Foscari”, Castello 2737/b 30122 Venezia, Italy 4Istituto di Ecologia e Biologia Ambientale, Universita di Urbino “Carlo Bo”, Via I. Maggetti 22, 61029 Urbino (PU), Italy
Biogeography and ecology of geographically distant populations of sibling Cryptocephalus leaf beetles
2020
Different populations of two closely related species, Cryptocephalusflavipes and C. bameuli, from western (Alps, Apennines and Pyrenees) and central Europe (Poland, Ukraine and Pannonia) were analysed. On the basis of DNA sequences from two genes, cox1 and ef1-alpha, distinctiveness of both species was confirmed. Nevertheless, possible hybrids were identified in Carpathian mountains. We found a significant genetic differentiation among populations of C. flavipes and C. bameuli from distant regions but a high genetic similarity between populations of C. bameuli from north and south of the Carpathians. Demographic estimates suggest a past population expansion in the case of C. bameuli and a r…
A method for taxonomic determination ofCandida albicans with DNA probes
1993
Determination of Candida species represents an important problem derived from the clinical implications of the species belonging to this genus. DNA probes have already been used for the epidemiology of Candida albicans, as well as for taxonomic analysis of Candida and other genera, although these probes are based on non-species-specific DNA sequences. In this work we carried out a 48-h assay, allowing the identification of C. albicans from clinical isolates, using DNA probes based on C. albicans LEU2 and URA3 genes. Another probe related to C. albicans SEC18 gene was shown not to be C. albicans specific.
Possible association of sudden infant death with partial complement C4 deficiency revealed by post-mortem DNA typing of HLA class II and III genes
1989
Based on evidence of an increased rate of respiratory infections in sudden infant death (SID) infants as well as the observation of familial occurrence, we analysed in a retrospective study class II and class II genes of the major histocompatibility complex in 40 cases of SID by Southern blot analysis of DNA obtained post mortem from tissue samples. In 24 cases, the parents were interviewed and confirmatory human lymphocyte antigen (HLA) and DNA typing was carried out. Using HLA-DR beta and -DQ beta probes, no evidence of an abnormal HLA-DR frequency distribution in SID infants was detected (P = 0.97). Using DNA probes for the tandemly arranged complement C4 and steroid 21-hydroxylase genes…
Differential enumeration and in situ localization of microorganisms in the hindgut of the lower termite Mastotermes darwiniensis by hybridization wit…
1999
We examined the abundance and spatial distribution of major phylogenetic groups of the domain Bacteria in hindguts of the Australian lower termite Mastotermes darwiniensis by using in situ hybridization with group-specific, fluorescently labeled, rRNA-targeted oligonucleotide probes. Between 32.0 +/- 7.2% and 52.3 +/- 8.2% of the DAPI-stained cells in different hindgut fractions were detected with probe EUB338, specific for members of the domain Bacteria. About 85% of the prokaryotic cells were associated with the flagellates of the thin-walled anterior region (P3a) and the thick wall of the posterior region (P3b/P4) of the hindgut, as shown by DAPI staining. At most, half of the EUB338-det…