Search results for "Hybridization"
showing 10 items of 812 documents
Early evolutionary colocalization of the nuclear ribosomal 5S and 45S gene families in seed plants: evidence from the living fossil gymnosperm Ginkgo…
2012
In seed plants, the colocalization of the 5S loci within the intergenic spacer (IGS) of the nuclear 45S tandem units is restricted to the phylogenetically derived Asteraceae family. However, fluorescent in situ hybridization (FISH) colocalization of both multigene families has also been observed in other unrelated seed plant lineages. Previous work has identified colocalization of 45S and 5S loci in Ginkgo biloba using FISH, but these observations have not been confirmed recently by sequencing a 1.8 kb IGS. In this work, we report the presence of the 45S–5S linkage in G. biloba, suggesting that in seed plants the molecular events leading to the restructuring of the ribosomal loci are much o…
The effect of inbreeding rate on fitness, inbreeding depression and heterosis over a range of inbreeding coefficients
2014
Understanding the effects of inbreeding and genetic drift within populations and hybridization between genetically differentiated populations is important for many basic and applied questions in ecology and evolutionary biology. The magnitudes and even the directions of these effects can be influenced by various factors, especially by the current and historical population size (i.e. inbreeding rate). Using Drosophila littoralis as a model species, we studied the effect of inbreeding rate over a range of inbreeding levels on (i) mean fitness of a population (relative to that of an outbred control population), (ii) within‐population inbreeding depression (reduction in fitness of offspring fro…
Data from: The effect of inbreeding rate on fitness, inbreeding depression and heterosis over a range of inbreeding coefficients
2014
Understanding the effects of inbreeding and genetic drift within populations and hybridization between genetically differentiated populations is important for many basic and applied questions in ecology and evolutionary biology. The magnitudes and even the directions of these effects can be influenced by various factors, especially by the current and historical population size (i.e., inbreeding rate). Using Drosophila littoralis as a model species, we studied the effect of inbreeding rate over a range of inbreeding levels on 1) mean fitness of a population (relative to that of an outbred control population), 2) within-population inbreeding depression (reduction in fitness of offspring from …
Data from: Genomics of speciation and introgression in Princess cichlid fishes from Lake Tanganyika
2016
How variation in the genome translates into biological diversity and new species originate has endured as the mystery of mysteries in evolutionary biology. African cichlid fishes are prime model systems to address speciation-related questions for their remarkable taxonomic and phenotypic diversity, and the possible role of gene flow in this process. Here, we capitalize on genome sequencing and phylogenomic analyses to address the relative impacts of incomplete lineage sorting, introgression and hybrid speciation in the Neolamprologus savoryi-complex (the ‘Princess cichlids’) from Lake Tanganyika. We present a time-calibrated species tree based on whole-genome sequences and provide strong ev…
Chromosomal assignment of the ovine hairless (hr) gene by fluorescence in situ hybridization.
2008
Congenital hypotrichosis in mammalian species consists of partial or complete absence of a hair coat at birth. Affected individuals having a partial hair coat at birth may loose it subsequently.The aim of this paper was to physically map the ovine hr gene using fluorescence in situ hybridization (FISH).A preliminary study on an internet data bank (http://www.informatics.jax.org) showed that the regions of the hr gene on murine chromosome 14 and human chromosome 8 present homology with ovine chromosome 2.
bbFISH-ing in the sonication fluid
2019
Abstract By 2030, the annual number of combined total hip and knee arthroplasty is estimated to reach 3.5 to 4 million in the US alone. In the context of a constant increase of the number of primary and revision total hip and knee arthroplasty, an increased risk of complication is expected. Prosthetic joint infections (PJIs) represent major cause of healthcare expenditure and morbidity. PJI still remain the most common and feared arthroplasty complication. A rapid and correct diagnosis of infection is decisive for a correct therapeutical management. In this setting, the Academic Emergency Hospital Sibiu adopted and implemented, with the beginning of September 2016, a new strategy for the di…
Absolute quantification of noncoding RNA by microscale thermophoresis
2019
Abstract Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization‐based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA‐Seq‐based quantification approaches, strongly suggesting a bias due to tRNA modifica…
Variants of human CLDN9 cause mild to profound hearing loss
2021
Hereditary deafness is clinically and genetically heterogeneous. We investigated deafness segregating as a recessive trait in two families. Audiological examinations revealed an asymmetric mild to profound hearing loss with childhood or adolescent onset. Exome sequencing of probands identified a homozygous c.475G>A;p.(Glu159Lys) variant of CLDN9 (NM_020982.4) in one family and a homozygous c.370_372dupATC;p.(Ile124dup) CLDN9 variant in an affected individual of a second family. Claudin 9 (CLDN9) is an integral membrane protein and constituent of epithelial bicellular tight junctions that form semi-permeable, paracellular barriers between inner ear perilymphatic and endolymphatic compartment…
(no title)
2017
Biological invasions are recognized as a significant evolutionary factor over short time scales. In particular, their effect is well recorded on the genetic structure of populations, the patterns of phenotypic evolution and the richness of parasite fauna associated to invasive populations. This study aims at quantifying the consequences of a biological invasion according to these three levels (genetical, phenotypical and parasitological) taking as example the Mozambique tilapia Oreochromis mossambicus. This African cichlid is characterized by an unusual conservation status since it is both (i) ranked among the world's worst invasive species due to its global dispersion during the 20th centu…
Julkiset palvelutuotantotehtävät suomalaisten liikuntaseurojen muodonmuutosten lähteenä
2022
Suomalaisessa yhteiskunnassa kansalaistoimijoilla on aina ollut merkittävä asema väestön hyvinvoinnin ja sosiaalisen turvallisuuden tuottamisessa. Viime vuosina julkinen sektori on kuitenkin pyrkinyt laajentamaan muiden yhteiskuntasektorien toimijoiden, kuten liikunta- ja urheiluseurojen, osallistumista kansalaisten palvelutuotantoon. Tarkastelemme artikkelissa, (1) millaisiin julkisten palvelujen tuotantotehtäviin liikuntaseurat ovat olleet valmiita sitoutumaan ja (2) miten tehtävät ovat muuttaneet seuratoimintaa. Lähestymme tutkimusaihettamme vertailemalla kahdeksaa julkiseen palvelutuotantoon paikallisesti sitoutunutta liikuntaseuraa. Tutkimusaineistomme koostuu tapaustutkimusseurojen to…