Search results for "I h"

A complete set of nascent transcription rates for yeast genes

2010

The amount of mRNA in a cell is the result of two opposite reactions: transcription and mRNA degradation. These reactions are governed by kinetics laws, and the most regulated step for many genes is the transcription rate. The transcription rate, which is assumed to be exercised mainly at the RNA polymerase recruitment level, can be calculated using the RNA polymerase densities determined either by run-on or immunoprecipitation using specific antibodies. The yeast Saccharomyces cerevisiae is the ideal model organism to generate a complete set of nascent transcription rates that will prove useful for many gene regulation studies. By combining genomic data from both the GRO (Genomic Run-on) a…

Genome-wide studies of mRNA synthesis and degradation in eukaryotes

2012

In recent years, the use of genome-wide technologies has revolutionized the study of eukaryotic transcription producing results for thousands of genes at every step of mRNA life. The statistical analyses of the results for a single condition, different conditions, different transcription stages, or even between different techniques, is outlining a totally new landscape of the eukaryotic transcription process. Although most studies have been conducted in the yeast Saccharomyces cerevisiae as a model cell, others have also focused on higher eukaryotes, which can also be comparatively analyzed. The picture which emerges is that transcription is a more variable process than initially suspected,…

Chromatin structure of the yeast FBP1 gene: transcription-dependent changes in the regulatory and coding regions.

1993

We have studied the chromatin structure of the Saccharomyces cerevisiae FBP1 gene, which codes for fructose-1,6-bisphosphatase. A strong, constitutive, DNase I, micrococcal nuclease and S1 nuclease hypersensitive site is present close to the 3′ end of the coding region. In the repressed state, positioned nucleosomes exist around this site, and subtle changes occur in this nucleosomal organization upon derepression. A DNase I hypersensitive region is located within the promoter between positions −540 and −400 and it extends towards the gene in the derepressed state, leading to an alteration of nucleosomal positioning. Psoralen crosslinking of chromatin, which is used for the first time to st…

Domestic violence against women as a reason to sanctification in Byzantini hagiography

2018

Aa. Dd.: La Germania a Xàtiva. Actes de les XI Jornades d'Art i Història. Xàtiva, 2019

2021

Like barn leker mest, men er det best? : Integrering av enslige mindreårige gjennom fritidsaktiviteter

2016

Master thesis development management - University of Agder 2016

Doctor Honoris Causa a Santiago Muñoz Machado

2013

El prestigiós jurista Santiago Muñoz Machado serà investit doctor `honoris causa´ per la Universitat de València, en un acte que se celebra al Paranimf de l?edifici històric de La Nau hui dijous, dia 7 de març a les 12 hores.

Conoscenze ed attitudini nei confronti della vaccinazione anti HPV delle mamme e delle vaccinate: risultati di un monitoraggio di strategie di preven…

2013

409 Using rai-hc assessment instrument to classify home care clients in Finland based on fire emergency evacuation capacity

2016

Background When an uncontrollable fire is detected in a building there may be a 2–3 minutes window for the occupants to move to safety. Poor physical function and cognition may become critical factors in an evacuation situation. In this study a novel functional scale measuring emergency evacuation capacity of home care clients is presented. Methods A fire security expert visited about 250 home care clients assessing their evacuation capacities. For the evacuation capacity three categories were used: 1) is able to evacuate; 2) may be able; 3) unable to evacuate. This data was linked to comprehensive assessments of clients functional and health status performed by home care nurses using the i…

An N-terminal deletion variant of HCN1 in the epileptic WAG/Rij strain modulates HCN current densities.

2015

Rats of the Wistar Albino Glaxo/Rij (WAG/Rij) strain show symptoms resembling human absence epilepsy. Thalamocortical neurons of WAG/Rij rats are characterized by an increased HCN1 expression, a negative shift in Ih activation curve, and an altered responsiveness of Ih to cAMP. We cloned HCN1 channels from rat thalamic cDNA libraries of the WAG/Rij strain and found an N-terminal deletion of 37 amino acids. In addition, WAG-HCN1 has a stretch of six amino acids, directly following the deletion, where the wild-type sequence (GNSVCF) is changed to a polyserine motif. These alterations were found solely in thalamus mRNA but not in genomic DNA. The truncated WAG-HCN1 was detected late postnatal …