Search results for "IAPR"
showing 10 items of 11 documents
SICILIAN NATURALISTIC NEWS: 11 Aclista alticollis; 12 Mycomya (Mycomya) prominens; 13 Empis (Leptempis) confusa; 14 Sciapus platypterus; 15 Myopa pic…
2023
SICILIAN NATURALISTIC NEWS: 11 Aclista alticollis; 12 Mycomya (Mycomya) prominens; 13 Empis (Leptempis) confusa; 14 Sciapus platypterus; 15 Myopa picta; 16 Diaea dorsata; 17 Franklinothrips megalops; 18 Dasyhelea bilineata; 19 Forcipomyia (Synthridomyia) murina; 20 Forcipomyia (Euprojoannisia) psilonota; 21 Incertana drepanensis
MOLECULAR BASIS OF DRUG PHOTOTOXICITY: PHOTOSENSITIZED CELL DAMAGE BY THE MAJOR PHOTOPRODUCT OF TIAPROFENIC ACID
1994
Tiaprofenic acid is a photosensitizing nonsteroidal anti-inflammatory drug, whose major photoproduct (decarboxytiaprofenic acid) is also a potent photosensitizer. Because of the lack of the carboxylate moiety, this photoproduct is more lipophilic and might bind more efficiently to cell membranes, thereby causing phototoxic damage. To verify the feasibility of this hypothesis, we have prepared the 3H-labeled analogs of tiaprofenic acid and its photoproduct and examined the binding, persistence and phototoxicity of the photoproduct using poorly metabolizing (fibroblasts) and actively metabolizing cells (hepatocytes). The photoproduct of tiaprofenic acid accumulates in both cell types as it is…
Photobinding of Tiaprofenic Acid and Subprofen to Proteins and Cells: A Combined Study Using Radiolabeling, Antibodies and Laser Flash Photolysis of …
1998
Drug photoallergy is a matter of current concern. It involves the formation of drug-protein photoadducts (photoantigens) that may ultimately trigger an immunological response. Tyrosine residues appear to be key binding sites in proteins. The present work has investigated the photobinding of tiaprofenic and (TPA) and the closely related isomer suprofen (SUP) to proteins and cells by means of radioactive labeling and drug-directed antibodies. To ascertain whether preassociation with the protein may play a role in photoreactivity, two model bichromophoric compounds (TPA-Tyr and SUP-Tyr) have been prepared and studied by laser flash photolysis. The results of this work show that (a) TPA and SUP…
Isolation of cross-coupling products in model studies on the photochemical modification of proteins by tiaprofenic acid
1999
To gain insight into the chemical nature of drug-induced photoallergy, model studies have been carried out on the photochemical modification of proteins by tiaprofenic acid. Irradiation of decarboxylated tiaprofenic acid (DTPA) in the presence of p-cresol leads to C–C- and C–O-connected p-cresol “dimers”, together with DTPA hydrodimers. The p-cresol–DTPA cross-coupling product was not detected in this reaction. However, a product of this type is formed using a more hindered phenol, such as 2,6-di-tert-butylphenol. Similar results are obtained when tiaprofenic acid (TPA) or its methyl ester are used as photosensitizers. The observed formation of “dimers” can be related to protein photo-cross…
Psykoterapiaprosessin keskeiset tekijät perheterapeuttien arvioitavina : Psychotherapy Process Q-set -menetelmän sovittaminen pariterapian tutkimiseen
2016
Tutkimuksessa selvitettiin Psychotherapy Process Q-set -menetelmän (PQS) ominaisuuksia ja sovellettavuutta pari- ja perheterapian näkökulmasta. Tarkastelu suoritettiin asiantuntija-arvioiden avulla kolmessa eri vaiheessa. Asiantuntijoina toimi joukko suomalaisia perheterapiakouluttajia. Ensimmäisessä vaiheessa asiantuntijat (n=8) arvioivat PQS:n kuvaamien yksilöpsykoterapiatekijöiden relevanssia pari- ja perheterapiassa. Toisessa vaiheessa asiantuntijat(n=10) arvioivat muokattujen PQS:n osioiden ja uusien pariterapiaosioiden merkitystä pariterapiaprosessin tutkimisessa. Kolmannessa vaiheessa käytettiin pientä asiantuntijaryhmää(n=5), jonka avulla etsittiin konsensusperiaatetta noudattaen sa…
DRM BLOG MAPPA DELLA RICERCA IN DESIGN: DESIGN DEI MATERIALI,DESIGN E MPI, DISTRETTI INDUSTRIALI.
2008
L'intervista riguarda la direzione di ricerca intrapresa dal dipartimento rilevando il contributo nel settore disciplinare per gli aspetti inerenti lo sviluppo sostenibile, la ripresa delle attività radicate nel contesto meridionale.
Salient Pixels and Dimensionality Reduction for Display of Multi/Hyperspectral Images
2012
International audience; Dimensionality Reduction (DR) of spectral images is a common approach to different purposes such as visualization, noise removal or compression. Most methods such as PCA or band selection use either the entire population of pixels or a uniformly sampled subset in order to compute a projection matrix. By doing so, spatial information is not accurately handled and all the objects contained in the scene are given the same emphasis. Nonetheless, it is possible to focus the DR on the separation of specific Objects of Interest (OoI), simply by neglecting all the others. In PCA for instance, instead of using the variance of the scene in each spectral channel, we show that i…
Antibodies directed to drug epitopes to investigate the structure of drug-protein photoadducts. Recognition of a common photobound substructure in ti…
2001
Drug-induced photoallergy is an immune adverse reaction to the combined effect of drugs and light. From the mechanistic point of view, it first involves covalent binding of drug to protein resulting in the formation of a photoantigen. Hence, determination of the structures of drug-protein photoadducts is of great relevance to understand the molecular basis of photoallergy and cross-immunoreactivity among drugs. Looking for new strategies to investigate the covalent photobinding of drugs to proteins, we generated highly specific antibodies to drug chemical substructures. The availability of such antibodies has allowed us to discriminate between the different modes by which tiaprofenic acid (…
Structure and Photochemical Behavior of the Cyclodextrin Inclusion Complexes of the Benzoylthiophene-Derived Drugs Tiaprofenic Acid (=5-Benzoyl-α-met…
2001
The effect of β-cyclodextrin (β-CD) on the excited-state reactivity of the two benzoylthiophene derivatives, tiaprofenic acid (TPA; 2) and suprofen (SPF; 3) in their carboxylate forms is studied. The presence of β-cyclodextrin does not affect the nature of the photoproduced transients and the photoproducts, but increases the photodegradation quantum yields of both drugs. The efficiency of the photodecarboxylation process is enhanced. This effect is rationalized in the light of the inclusion of 2 and 3 in the β-CD cavity, affecting the energy of the lowest excited states of the drugs. The structure of the complexes is determined by induced circular dichroism, and molecular-mechanics and dyna…
Mechanisms of photosensitization by drugs: Involvement of tyrosines in the photomodification of proteins mediated by tiaprofenic acid in vitro
1997
The photosensitizing potential of drugs must be related to their photoreactivity towards the target biomolecules. In this context, a representative photosensitizing drug (tiaprofenic acid) was co-irradiated with a model protein, bovine serum albumin (BSA). This led to a significant degree of protein crosslinking and to the formation of trace amounts of drug-BSA photoadducts. Amino acid analysis of the hydrolysed (HC1) protein showed that His and Tyr undergo a dramatic decrease (approx. 90%) as a consequence of drug-mediated photodynamic processes. When the drug was irradiated in the presence of the pure amino acids, extensive phototransformation of the latter was observed. Other photosensit…