Search results for "IATE"
showing 10 items of 4252 documents
The effects of two-way communication and chat service usage on consumer attitudes in the e-commerce retailing sector
2018
In e-commerce retailing (e-retailing), where competitors are only one click away and prices are easy to compare, providing superior customer service and reciprocal communication via a company’s website are important aspects of attracting and retaining customers. One increasingly popular solution to improve customer service is a “live chat” interface that allows consumers to have real-time conversations online with customer service agents. As the literature on the impacts of real-time communication via live chat is currently very limited, this study develops and tests a model that demonstrates the moderating effects of a chat service on the relationship between two-way communication (i.e., a…
Tulokkaasta tuottavaksi asiantuntijaksi : perehdyttäminen kehittämisen välineenä eräissä suomalaisissa tietoalan yrityksissä
2010
Virtudes escolares percibidas por estudiantes y docentes ecuatorianos: diferencias según sexo, establecimiento y roles
2020
El objetivo del estudio es evaluar la virtuosidad percibida en organizaciones escolares y las diferencias por tipo de centro educativo (fiscal, municipal); sexo (mujer, hombre) y rol (estudiantes, docentes). Se trató de un estudio instrumental basado en el análisis de la estructura factorial y la fiabilidad de un cuestionario para evaluar virtudes percibidas en escuelas (VPOE); y, de un estudio descriptivo transversal que analizó diferencias por sexo, rol y tipo de organizaciones escolares con relación a las virtudes percibidas. Se trabajó con una muestra de 550 estudiantes (49.28 % mujeres) y 105 docentes (47.62% mujeres). El análisis factorial determinó un modelo de cuatro factores que pe…
Mouse models of cytomegalovirus latency: overview.
2002
Abstract Background: The molecular regulation of viral latency and reactivation is a central unsolved issue in the understanding of cytomegalovirus (CMV) biology. Like human CMV (hCMV), murine CMV (mCMV) can establish a latent infection in cells of the myeloid lineage. Since mCMV genome remains present in various organs after its clearance from hematopoietic cells first in bone marrow and much later in blood, there must exist one or more widely distributed cell type(s) representing the cellular site(s) of enduring mCMV latency in host tissues. Endothelial cells and histiocytes are candidates, but the question is not yet settled. Another long debated problem appears to be solved: mCMV establ…
Genomic structure and promoter analysis of pathogen-inducedrepatgenes fromSpodoptera exigua
2009
The repat gene family encodes midgut proteins overexpressed in response to pathogen infection in the lepidopteran Spodoptera exigua. Up-regulation of repat genes has been observed after challenging the larvae with both Bacillus thuringiensis toxins and after infection with the baculovirus Autographa californica multiple nucleopolyhedrovirus. In our study, PCR amplification of the genomic region and genome walking were used to obtain the genomic structure and the sequence of the 5'-upstream region of repat1 and repat2, two of the most phylogenetically distant members of the repat family. A similar gene structure between repat1 and repat2 has been found, with conserved exon-intron positions a…
Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.
2004
ABSTRACT Recent reports suggest that nuclear domain(s) 10 (ND10) is the site of papillomavirus morphogenesis. The viral genome replicates in or close to ND10. In addition, the minor capsid protein, L2, accumulates in these subnuclear structures and recruits the major capsid protein, L1. We have now used cell lines deficient for promyelocytic leukemia (PML) protein, the main structural component of ND10, to study the role of this nuclear protein for L2 incorporation into virus-like particles (VLPs). L2 expressed in PML protein knockout (PML −/− ) cells accumulated in nuclear dots, which resemble L2 aggregates forming at ND10 in PML protein-containing cells. These L2 assemblies also attracted…
Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry
2013
ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.
Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells
2007
Baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), has the ability to transduce mammalian cell lines without replication. The general objective of this study was to detect the transcription and expression of viral immediate-early genes in human cells and to examine the interactions between viral components and subnuclear structures. Viral capsids were seen in large, discrete foci in nuclei of both dividing and non-dividing human cells. Concurrently, the transcription of viral immediate-early transregulator genes (ie-1, ie-2) and translation of IE-2 protein were detected. Quantitative microscopy imaging and analysis showed that virus transduction altered the size of …
Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry
2012
ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…
Conflicts in workplace in technology-mediated communication
2017
Konfliktit työyhteisön teknologiavälitteisessä vuorovaikutuksessa. Tämän tutkielman tavoitteena oli kuvata työntekijöiden havaintoja konflikteista, jotka ilmenivät työpaikkojen teknologiavälitteisessä vuorovaikutuksessa. Työntekijöiden havaintoihin pohjaten tutkielma pyrki kasvattamaan ymmärrystä työelämässä ja teknologiavälitteisessä vuorovaikutuksessa ilmenevistä konflikteista etsimällä vastauksia siihen, millaisia syitä, seurauksia ja lopputuloksia konflikteilla on työyhteisöjen teknologiavälitteisessä vuorovaikutuksessa. Konfliktien ja niiden hallintakeinojen ymmärtäminen on tärkeää työyhteisöille ja niissä toimiville työyhteisön jäsenille. Tutkielman menetelmänä käytettiin teoriasidonn…