Search results for "IL-2"

showing 10 items of 267 documents

Regulatory T Cells Accumulate and Proliferate in the Ischemic Hemisphere for up to 30 Days after MCAO

2012

Local and peripheral immune responses are activated after ischemic stroke. In our present study, we investigated the temporal distribution, location, induction, and function of regulatory T cells (Tregs) and the possible involvement of microglia, macrophages, and dendritic cells after middle cerebral artery occlusion (MCAO). C57BL/6J and Foxp3EGFP transgenic mice were subjected to 30 minutes MCAO. On days 7, 14, and 30 after MCAO, Tregs and antigen presenting cells were analyzed using fluorescence activated cell sorting multicolor staining and immunohistochemistry. A strong accumulation of Tregs was observed on days 14 and 30 in the ischemic hemisphere accompanied by the elevated presence …

CD4-Positive T-LymphocytesGenetically modified mousePathologymedicine.medical_specialtyTime FactorsAntigen-Presenting CellsMice Transgenicchemical and pharmacologic phenomenaLymphocyte ActivationT-Lymphocytes RegulatoryNeuroprotectionFlow cytometryMice03 medical and health sciences0302 clinical medicineImmune systemGenes ReportermedicineAnimalsLymphocyte CountIL-2 receptorAntigen-presenting cellCell Proliferation030304 developmental biologyHomeodomain Proteins0303 health sciencesmedicine.diagnostic_testMicrogliabusiness.industryInterleukin-2 Receptor alpha SubunitFOXP3Forkhead Transcription FactorsInfarction Middle Cerebral Arteryhemic and immune systemsFlow CytometryImmunohistochemistryMice Inbred C57BLmedicine.anatomical_structureNeurology030220 oncology & carcinogenesisImmunologyOriginal ArticleNeurology (clinical)CorrigendumCardiology and Cardiovascular Medicinebusiness030217 neurology & neurosurgeryJournal of Cerebral Blood Flow & Metabolism
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Rapid identification and sorting of viable virus-reactive CD4+ and CD8+ T cells based on antigen-triggered CD137 expression

2008

Abstract Current methods for the detection and isolation of antigen-specific CD4 + and CD8 + T cells require the availability of peptide/MHC multimers or are restricted to cells that produce cytokines after antigen contact. Here we show that de novo cell surface expression of the TNF-receptor family member CD137 (4-1BB) identifies recently activated, but not resting, human CD4 + and CD8 + memory T cells. Maximum CD137 expression level is uniformly observed in both T-cell subsets at 24h after stimulation with antigen. In experiments with CMV and EBV-reactive T cells, we confirmed the specificity of CD137 expression by co-staining with peptide/HLA tetramers. Substantial proportions of CD137 +…

CD4-Positive T-LymphocytesHerpesvirus 4 HumanImmunologyCytomegalovirusStreptamerCD8-Positive T-LymphocytesLymphocyte ActivationViral Matrix ProteinsInterferon-gammaTumor Necrosis Factor Receptor Superfamily Member 9Interleukin 21HumansImmunology and AllergyCytotoxic T cellIL-2 receptorAntigen-presenting cellAntigens ViralCD40biologyImmunomagnetic SeparationCD28PhosphoproteinsNatural killer T cellAdoptive TransferMolecular biologyGene Expression Regulationbiology.proteinK562 CellsJournal of Immunological Methods
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Regulatory activity of human CD4 CD25 T cells depends on allergen concentration, type of allergen and atopy status of the donor.

2005

Regulatory CD4+ CD25+ FoxP3-positive T cells (Treg) are functional in most atopic patients with allergic rhinitis and are able to inhibit T helper type 1 (Th1) and Th2 cytokine production of CD4+ CD25- T cells. This study was designed to analyse the following additional aspects: influence of allergen concentration, influence of the type of allergen, and influence of the atopy status of the donor on the strength of the regulatory activity. CD4+ CD25- T cells from healthy non-atopic controls or from grass-pollen-allergic or wasp-venom-allergic donors were stimulated alone or in the presence of Treg with autologous mature monocyte-derived dendritic cells which were pulsed with different concen…

CD4-Positive T-LymphocytesHypersensitivity ImmediateAllergymedicine.medical_treatmentImmunologyDose-Response Relationship Immunologicchemical and pharmacologic phenomenaWasp VenomsReceptors Nerve Growth FactorBiologymedicine.disease_causePoaceaeReceptors Tumor Necrosis FactorAtopyInterleukin 21AllergenTh2 CellsAntigenT-Lymphocyte SubsetsGlucocorticoid-Induced TNFR-Related ProteinmedicineImmunology and AllergyHumansIL-2 receptorReceptorCells CulturedCell Proliferationhemic and immune systemsForkhead Transcription FactorsReceptors Interleukin-2Original ArticlesAllergensTh1 Cellsmedicine.diseaseCoculture TechniquesCytokineImmunologyCytokinesPollenImmunology
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Modification of the human allergic immune response by allergen-DNA-transfected dendritic cells in vitro.

2004

Abstract Background Atopic-allergic diseases are characterized by T H 2-dominated immune responses, resulting in IgE production. DNA-based immunotherapies have been shown to shift the immune response toward a T H 1-type response in animal models. Objective The aim of the study was to analyze whether dendritic cells (DCs) transfected with allergen-DNA conjugates are able to stimulate human autologous CD4 + T cells, CD8 + T cells, or both from atopic individuals to produce T H 1 cytokines instead of T H 2 cytokines. Methods For this purpose, human mature DCs from atopic donors were transfected with an adenovirus encoding the allergen Phl p 1. Autologous CD4 + and CD8 + T cells were stimulated…

CD4-Positive T-LymphocytesHypersensitivity Immediatemedicine.medical_treatmentImmunologyGenetic Vectorschemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesLymphocyte ActivationTransfectionInterleukin 21Interferon-gammaImmune systemmedicineImmunology and AllergyCytotoxic T cellHumansIL-2 receptorAntigen-presenting cellCells CulturedPlant ProteinsAdenoviruses HumanDendritic cellDendritic CellsAllergensTh1 CellsMolecular biologyCytokineImmunologyCytokinesCD8The Journal of allergy and clinical immunology
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Direct Cellular Interaction with Activated CD4+T Cells Overcomes Hyporesponsiveness of B-Cell Chronic Lymphocytic Leukemiain Vitro

1998

The proliferative response of clonal B cells from patients with chronic lymphocytic leukemia (B-CLL) is drastically reduced compared to normal B lymphocytes stimulated via the B cell antigen receptor complex or by CD40 ligation. In the present study we demonstrate that hyporesponsiveness of CLL-B cells can be overcome by stimulatory pathways mediated by activated CD4(+) T cells. In contrast to CD40 ligation, costimulation with activated T cells promotes a proliferative response in CLL-B cells identical to that in normal B cells. Furthermore, coculture with activated T cells improved survival of CLL-B cells in vitro. Differentiation of CLL-B cells into IgM producing cells was promoted, as we…

CD4-Positive T-LymphocytesImmunologyB-cell receptorLymphocyte ActivationInterleukin 21Antigens CDhemic and lymphatic diseasesHumansCytotoxic T cellIL-2 receptorCD40 AntigensAntigen-presenting cellCells CulturedB-LymphocytesCD40biologyZAP70Cell DifferentiationLeukemia Lymphocytic Chronic B-CellCell biologyB-1 cellImmunoglobulin MAntigens Surfacebiology.proteinInterleukin-2Cell DivisionCellular Immunology
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Cutting Edge: TGF-β Induces a Regulatory Phenotype in CD4+CD25− T Cells through Foxp3 Induction and Down-Regulation of Smad7

2004

Abstract CD4+CD25+ regulatory cells are a subpopulation of T lymphocytes of thymic origin. However, recent data suggest an alternative commitment of regulatory T cells in the periphery, although the precise mechanism is unknown. In the present work, we demonstrate that TGF-β is able to induce Foxp3 expression and subsequently a regulatory phenotype in CD4+CD25− peripheral murine T cells. Similarly, TGF-β induced Foxp3 in human CD4+CD25− T cells. Moreover, we show that the inhibitory Smad7 protein that is normally induced by TGF-β and limits TGF-β signaling, is strongly down-regulated by Foxp3 at the transcriptional level. Foxp3-mediated down-regulation of Smad7 subsequently rendered CD4+CD2…

CD4-Positive T-LymphocytesImmunologyDown-Regulationchemical and pharmacologic phenomenaThymus GlandBiologyImmunophenotypingSmad7 ProteinMiceInterleukin 21Downregulation and upregulationT-Lymphocyte SubsetsTransforming Growth Factor betaTGF beta signaling pathwayAnimalsHumansImmunology and AllergyCytotoxic T cellIL-2 receptorCells CulturedZAP70FOXP3Cell DifferentiationForkhead Transcription FactorsReceptors Interleukin-2hemic and immune systemsPhenotypeCell biologyDNA-Binding ProteinsTrans-ActivatorsSpleenSignal TransductionThe Journal of Immunology
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Differential Regulatory Capacity of CD25+ T Regulatory Cells and Preactivated CD25+ T Regulatory Cells on Development, Functional Activation, and Pro…

2004

Abstract CD25+ T regulatory (Treg) cells play a central role regarding the maintenance of peripheral tolerance via suppression of autoaggressive CD4+ T cells, CD8+ T cells, and Th1 cells. In this study we demonstrate that CD25+ Treg cells can also suppress the differentiation of murine conventional CD4+ T cells toward Th2 cells in a contact-dependent manner. However, the cytokine production and proliferation of established Th2 cells could not be inhibited by freshly isolated CD25+ Treg cells, whereas a strong inhibition of differentiated Th2 cells by in vitro preactivated CD25+ Treg cells could be observed. Inhibition of both conventional CD4+ T cells and Th2 cells is accompanied by a stron…

CD4-Positive T-LymphocytesImmunologySuccinimideschemical and pharmacologic phenomenaLymphocyte ActivationMiceInterleukin 21Th2 CellsT-Lymphocyte SubsetsAnimalsImmunology and AllergyCytotoxic T cellIL-2 receptorAntigen-presenting cellInterleukin 3Mice Inbred BALB CCD40biologyPeripheral toleranceForkhead Transcription FactorsReceptors Interleukin-2hemic and immune systemsFluoresceinsCell biologyDNA-Binding ProteinsMice Inbred C57BLbiology.proteinInterleukin 12CytokinesThe Journal of Immunology
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The receptor NLRP3 is a transcriptional regulator of TH2 differentiation.

2015

The receptor NLRP3 is involved in the formation of the NLRP3 inflammasome that activates caspase-1 and mediates the release of interleukin 1β (IL-1β) and IL-18. Whether NLRP3 can shape immunological function independently of inflammasomes is unclear. We found that NLRP3 expression in CD4(+) T cells specifically supported a T helper type 2 (TH2) transcriptional program in a cell-intrinsic manner. NLRP3, but not the inflammasome adaptor ASC or caspase-1, positively regulated a TH2 program. In TH2 cells, NLRP3 bound the Il4 promoter and transactivated it in conjunction with the transcription factor IRF4. Nlrp3-deficient TH2 cells supported melanoma tumor growth in an IL-4-dependent manner and …

CD4-Positive T-LymphocytesInflammasomesImmunologyBlotting WesternBiologyInterleukin 21MiceTh2 CellsCell Line TumorNLR Family Pyrin Domain-Containing 3 ProteinImmunology and AllergyCytotoxic T cellAnimalsIL-2 receptorPromoter Regions GeneticInterleukin 3Oligonucleotide Array Sequence AnalysisMice KnockoutCD40integumentary systemReverse Transcriptase Polymerase Chain ReactionZAP70Gene Expression ProfilingCell DifferentiationNeoplasms ExperimentalAsthmaCell biologyGene Expression Regulation NeoplasticMice Inbred C57BLInterleukin 10Interferon Regulatory FactorsInterleukin 12biology.proteinNIH 3T3 CellsTrans-ActivatorsFemaleInterleukin-4Carrier ProteinsProtein BindingSignal TransductionNature immunology
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CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated human dendritic cells display antigen-specific suppressor activity.

2002

Interleukin-10 (IL-10)–treated dendritic cells (DCs) induce an alloantigen- or peptide-specific anergy in various CD4+ and CD8+ T-cell populations. In the present study, we analyzed whether these anergic T cells are able to regulate antigen-specific immunity. Coculture experiments revealed that alloantigen-specific anergic CD4+ and CD8+ T cells suppressed proliferation of syngeneic T cells in a dose-dependent manner. The same effect was observed when the hemagglutinin-specific CD4+T-cell clone HA1.7 or tyrosinase-specific CD8+ T cells were cocultured with anergic T cells of the same specificity. Anergic T cells did not induce an antigen-independent bystander inhibition. Suppression was depe…

CD4-Positive T-LymphocytesIsoantigensImmunoconjugatesImmunologyAntigen-Presenting Cellschemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesLymphocyte ActivationBiochemistryT-Lymphocytes RegulatoryAbataceptInterleukin 21Antigens CDAntigens NeoplasmCytotoxic T cellHumansCTLA-4 AntigenIL-2 receptorLeukapheresisAntigen-presenting cellMelanomaCells CulturedClonal AnergyImmunosuppression TherapyMonophenol MonooxygenaseCD28Cell BiologyHematologyDendritic cellT lymphocyteDendritic CellsNatural killer T cellAntigens DifferentiationCoculture TechniquesCell biologyInterleukin-10ImmunologyCD4 AntigensLeukocytes MononuclearCell DivisionBlood
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Murine liver antigen presenting cells control suppressor activity of CD4+CD25+ regulatory T cells.

2005

CD4(+)CD25(+) regulatory T cells (Treg) are important mediators of peripheral immune tolerance; however, whether Treg participate also in hepatic immune tolerance is not clear. Therefore, we tested the potential of Treg to suppress stimulation of CD4(+) T cells by liver sinusoidal endothelial cells (LSEC), Kupffer cells (KC), or hepatocytes. In the absence of Treg, all 3 types of liver cells could stimulate CD4(+) T cell proliferation; in the presence of Treg, however, CD4(+) T cell proliferation was suppressed. Interaction with KC even stimulated the expansion of the Treg population; LSEC or hepatocytes, in contrast, could not induce proliferation of Treg. Because liver inflammation can be…

CD4-Positive T-LymphocytesLiver cytologyKupffer CellsT cellT-LymphocytesAntigen-Presenting Cellschemical and pharmacologic phenomenaBiologyImmune toleranceMiceAntigenmedicineImmune ToleranceAnimalsIL-2 receptorAntigen-presenting cellCell ProliferationInflammationHepatologyLiver cellKupffer cellEndothelial Cellshemic and immune systemsReceptors Interleukin-2medicine.anatomical_structureLiverImmunologyCancer researchHepatocytesHepatology (Baltimore, Md.)
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