Search results for "IN VITRO CULTURE"

Oxidative stress in olive in vitro culture: effects of organized development and mass propagation of Biancolilla and Cerasuola cvs.

2006

The in vitro culture of olive explants very often results in poor growth and reduced proliferation of axillary buds. Many different factors have been considered and in many cases the unusual, for this species, surrounding environment has been held responsible for the scarce results obtained. Recently oxidative stresses have also been investigated and among the different factors involved, wounding, sterilisation, nutritional components and culture environment were claimed as main stress-inducing agents. This experiment reports on a preliminary study on two important Sicilian varieties, Biancolilla and Cerasuola, aiming to find the existing relationships among olive explant growth and prolife…

In vitro establishment and culture of two Sicilian cultivars of Olea europaea L

2012

Sicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtai…

In vitro rooting and acclimatization of Sicilian Hypericum perforatum

2015

Hypericum perforatum L. is traditionally used as a medicinal plant because of different bioactive compounds with documented antidepressant and anti-inflammatory activities. Plantlets mass production with high content of these secondary metabolites has been enhanced through in vitro culture but often the process has been stopped at the multiplication phase. A study was conducted in order to set up an efficient in vitro rooting and acclimatization protocol of a H. perforatum Sicilian genotype well-adapted to south Mediterranean conditions. Aseptic nodal segments were cultured on to a Murashige and Skoog (MS) basal medium supplemented with 4.44 μM 6-benzyladenine (BA) for multiplication. Micro…

Correlating stress and somatic embryogenesis for NBT: myth or reality?

2018

Global warming and growing demography have increased demand for agronomic resources, leading to increasing lack of land suitable for agriculture and provoking several abiotic stresses which, added to biotic ones, result in physiological and metabolic disorders that impact on crop yield when most needed. Reducing this impact is a major scientific and agronomic challenge and biotechnology would be an efficient alternative. However, to reduce risks of somaclonal variation among regenerants it is better to produce them by somatic embryogenesis directly from explants or gametes, or indirectly from callus or cell suspensions. Thus, globular embryos regenerate and develop through to the heart, tor…

Ploidy manipulation for genetic improvement in some Mediterranean fruit crops

2017

Plant breeding is focused on selection of new genotypes with improved traits. Conventional methods based on hybridization and those based on biotechnology (somatic hybridization, genetic transformation, ploidy manipulation, etc.) are used to create novel genetic variations. Biotechnology provides powerful tools for plant breeding, for instance, haploid technology allows achievement of homozygous lines from heterozygous parents in one step, which reduces significantly the time required by conventional methods. Concerning woody species, characterized by self-incompatibility, long juvenile period and high degree of heterozygosity, this technique is the only way to get homozygous lines. Haploid…

Somatic embryogenesis and flow cytometric assessment of nuclear genetic stability for Sansevieria spp.: an approach for in vitro regeneration of orna…

2023

Sansevieria Thunb. species are traditionally known as succulent ornamental plants worldwide. They are also cultivated for medicinal, fodder, soil conservation and fiber uses, and for their capacity to reduce environmental pollution. Sansevieria sexual propagation is limited by the lack of viable seeds, and reproduction is largely made via vegetative propagation by suckers or cuttings. For these reasons, genetic improvement by conventional breeding is limited. To overcome this problem and to address the increasing demand from customers for novel Sansevieria varieties, many commercial companies regularly use in vitro propagation, as is the case in the breeding process of several ornamental pl…

Oral Mucosa of celiac disease patients produces anti-endomysial and anti-transglutaminase antibodes. Diagnostic usefulness of an in vitro culture sys…

2007

Micropropagazione di Thymus capitatus

2011

Thymus capitatus is a compact woody shrub, endemic to the Mediterranean region, with attractive pink flowers. Although conventional vegetative propagation of T. capitatus is currently used, micropropagation could be effective for rapid mass propagation of selected clones and would preserve wild plants. Shoot tips of T. capitatus cultured on a gelled MS medium, supplemented with 0.4 mg l-1 2-iP, were used as primary explants to establish in vitro culture. Axillary shoots produced on uncontaminated explants were excised and subcultured to increase the stock of shoot cultures. To determine the optimal medium for shoot multiplication, increasing concentrations (0, 0.4, 0.8, 1.6 e 3.2 mg l-1) of…

Research on conversion of Carrizo citrange [C. sinensis (L.) Osb. × P. trifoliata (L.) Raf.] synthetic seeds.

2008

Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

2021

Cryopreservation by droplet vitrification was applied to hazelnut (Corylus avellana L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from in vitro growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced…