Search results for "INFECTIONS"

Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (R…

2015

The bacterium causing cholera, Vibrio cholerae, is a marine organism and coastal waters are important reservoirs of the organism. There are more than 200 serogroups of V. cholerae, of which serogroups O1 and O139 are known to be the causative agent of the cholera. The main virulent factor in V. cholerae is cholera toxin gene (ctx) that is found from the epidemic O1 and O139 strains, but may also be found in some strains other than O1 and O139 (non-O1 and non-O139). In this study, 48 V. cholerae strains isolated from three estuaries of Tanzania and 20 stool isolates were characterized in terms of their serogroups and possession of ctx gene and then compared using two PCR based fingerprinting…

Evaluation of the different exposure parameters for the accurate diagnosis of peri-implantitis severity in digital panoramic radiography.

2022

To evaluate the accuracy of the diagnosis of peri-implant bone defects? severities in digital panoramic radiographs obtained at different tube voltage and/or tube current settings. Two different sizes of peri-implant bone defects (type 1 and type 2) were prepared after the implants were inserted into 29 bovine rib blocks. Digital panoramic radiographs were obtained at eight different tube voltage and/or tube current settings for all rib blocks. Implant images were cropped separately. The average intensity value (AIV) of cropped images were analyzed using Adobe Photoshop CC software. The Kruskal-Wallis H test was used to compare AIVs. All cropped images were evaluated using a five-point Like…

Double copies of blaKPC-3::Tn4401a on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy

2015

ABSTRACT A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla KPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla KPC-3 gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla KPC-3 ::Tn 4401a caused by intramolecular transposition events were detected in the plasmid.

Chorobotwórczość pałeczki ropy błękitnej Pseudomonas aeruginosa w chorobach uszu i oczu

2016

Mast cells as protectors of health.

2019

Mast cells (MCs), which are well known for their effector functions in T(H)2-skewed allergic and also autoimmune inflammation, have become increasingly acknowledged for their role in protection of health. It is now clear that they are also key modulators of immune responses at interface organs, such as the skin or gut. MCs can prime tissues for adequate inflammatory responses and cooperate with dendritic cells in T-cell activation. They also regulate harmful immune responses in trauma and help to successfully orchestrate pregnancy. This review focuses on the beneficial effects of MCs on tissue homeostasis and elimination of toxins or venoms. MCs can enhance pathogen clearance in many bacter…

Evolution of nurses' social representation of hospital hygiene: From training to practice

2011

Nurses' social representations of hospital hygiene were analyzed. A cross-sectional method was used to compare and analyze the social representations of the concept domain of "hospital hygiene" across three independent groups of participants (N=744). The groups included: (a) professional nurses (N=114); (b) student nurses (N=315); and (c) psychology students (N=315). Comparisons were drawn: (a) between professionals and students; and (b) between student nurse cohorts at three different levels (years) of training. The results show an ongoing evolution of the social representations of hospital hygiene during training and in the course of professional practice. The representation of hospital h…

Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli

2005

Type 1 fimbriae ofEscherichia colifacilitate attachment to the host mucosa and promote biofilm formation on abiotic surfaces. The transcriptional regulator LrhA, which is known as a repressor of flagellar, motility and chemotaxis genes, regulates biofilm formation and expression of type 1 fimbriae. Whole-genome expression profiling revealed that inactivation oflrhAresults in an increased expression of structural components of type 1 fimbriae.In vitro, LrhA bound to the promoter regions of the twofimrecombinases (FimB and FimE) that catalyse the inversion of thefimApromoter, and to the invertible element itself. TranslationallacZfusions with these genes and quantification offimEtranscript le…

In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass

2019

© 2019 by the authors.

Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells.

2022

Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporo…

CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…

2021

Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…