Search results for "INOS"

Einflu� einer Hyperglyk�mie auf die Sauerstoff- und Glucoseaufnahme von Implantationstumoren (DS-Carcinosarkom) in vivo

1972

Um den Einflus erhohter arterieller Glucosekonzentrationen auf die Glucose- und Sauerstoffaufnahme von Tumorgewebe unter in vivo-Bedingungen zu bestimmen, wurden Untersuchungen an gewebsisolierten Implantationstumoren von Ratten (DS-Carcinosarkom) durchgefuhrt.

Encapsulation of in vitro-derived propagules of two genotypes of Capparis spinosa (L.) from Pantelleria Island

2020

Encapsulation technology, combining the advantages of zygotic or gamic seed with those of micropropagation, has recently attracted the interest of researchers as a new propagation approach, mainly due to the unsatisfying results of the traditional propagation strategies. The encapsulation of uninodal microcuttings (3-4 mm long) from two Sicilian Capparis spinosa (L.) genotypes (from Pantelleria Island: 'Tracino' and 'Scauri') was evaluated, observing the influence of the calcium alginate coating and of three different growth regulators (PGRs): 6-benzylaminopurine (BAP), meta-topolin (MT) and zeatin (ZEA), on viability, regrowth and conversion of the propagules. Caper microcuttings were diss…

Selection and micropropagation of valuable caper genotypes

2020

The high quality of the various biotypes present in the natural or cultivated state is one of the main features for caper production. Up to now, however, no selection activity has been carried out in order to identify the most suitable accessions for providing better quality products. In this paper, we report the first results of a selection of caper genotypes characterised by significant qualitative traits. A micropropagation protocol was evaluated in order to improve and allow the multiplication of the most promising Capparis spinosa L. subsp. spinosa genotypes, selected in Salina (Aeolian Islands), Sicily (Italy), in collaboration with the most important local growers.

Micromorphological observations on leaf and pollen ofCapparisL. sect.Capparis(Capparaceae)

2004

Sect. Capparis is represented by a single species, Capparis spinosa L., divided into several intraspecific taxa showing plesiomorphic features and disjunct distributions in the Old World. Leaf surface and pollen features were investigated in the whole group by SEM and light microscope observations. The section is characterized by simple hairs, a reticulate to undulate cuticle, anomocytic stomata surrounded by a peristomal rim, and trizonocolporate, prolate pollen grains. The characteristics of the indumentum appear constant, while the studied taxa are fairly differentiated with respect to cuticular patterns and dimensions of the stomata, and show slight differences in pollen size and exine …

Aspidin PB, a phloroglucinol derivative, induces apoptosis in human hepatocarcinoma HepG2 cells by modulating PI3K/Akt/GSK3β pathway.

2012

Aspidin PB, a phloroglucinol derivative isolated from Dryopteris fragrans (L.) Schott, has been previously reported to exert high biological activities. In the present study, we analyzed the apoptotic mechanisms of aspidin PB on human hepatoma cell line, HepG2. Initially, aspidin PB was shown to inhibit the growth of HepG2 cells in a time and dose-dependent manner. After treatment with aspidin PB for 72 h, 48 h and 24 h using MTT assay, the IC(50) values were 10.59 μM, 20.86 μM and 46.59 μM, respectively. Aspidin PB was capable to induce apoptosis, as measured by mitochondrial membrane potential (ΔΨm), acridine orange (AO) staining and propidium iodide (PI)/annexin V-FITC double staining. T…

Carcinosarcoma of the parotid gland with osteosarcomatous differentiation: a case report

2020

Noticia de las fiestas celebradas por la M.I.N. y L. Villa de Reynosa en la proclamacion del señor Rey Don Cárlos IV.

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Role of IP3 Receptors in Shaping the Carotid Chemoreceptor Response to Hypoxia but not to Hypercapnia in the Rat Carotid Body: An Evidence Review

2021

This article addresses the disparity in the transduction pathways for hypoxic and hypercapnic stimuli in carotid body glomus cells. We investigated and reviewed the experimental evidence showing that the response to hypoxia, but not to hypercapnia, is mediated by 1,4,5-inositol triphosphate receptors (IP3R/s) regulating the intracellular calcium content [Ca2+]c in glomus cells. The rationale was based on the past observations that inhibition of oxidative phosphorylation leads to the explicit inhibition of the hypoxic chemoreflex. [Ca2+]c changes were measured using cellular Ca2+-sensitive fluorescent probes, and carotid sinus nerve (CSN) sensory discharge was recorded with bipolar electrode…

Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.

2016

Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters…

In vitro efficacy of a Hydrophilic Central Venous Catheter Loaded with Silver to Prevent Microbial Colonization

1998

A method was developed to load the surface of a central venous catheter with silver to prevent bacterial colonization. Silver confers a broad antimicrobial activity with a relatively low risk of resistance. Catheters were incubated with a silver nitrate solution in different concentrations. The solvent, incubation temperature and incubation period were varied to examine the influence on the catheter loading. With increasing incubation temperature, time and concentration of silver nitrate, higher rates of silver elution were observed by atomic absorption spectroscopy. Furthermore, by using ethanol-water as a solvent instead of pure water, the amount of silver bound to the catheter surface wa…