Search results for "INTERFACE"

Adsorption and Conformation Behavior of Biotinylated Fibronectin on Streptavidin-Modified TiOX Surfaces Studied by SPR and AFM

2011

It is well-known that protein-modified implant surfaces such as TiO(2) show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiO(X) surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformati…

Reactive Surface Coatings Based on Polysilsesquioxanes: Controlled Functionalization for Specific Protein Immobilization

2009

The key designing in reliable biosensors is the preparation of thin films in which biomolecular functions may be immobilized and addressed in a controlled and reproducible manner. This requires the controlled preparation of specific binding sites on planar surfaces. Poly(methylsilsesquioxane)-poly(pentafluorophenyl acrylates) (PMSSQ-PFPA) are promising materials to produce stable and adherent thin reactive coatings on various substrates. Those reactive surface coatings could be applied onto various materials, for example, gold, polycarbonate (PC), poly(tetrafluoroethylene) (PTFE), and glass. By dipping those substrates in a solution of a desired amine, specific binding sites for protein ads…

Layer-by-Layer Assembly of a Streptavidin–Fibronectin Multilayer on Biotinylated TiOX

2013

The biomodification of surfaces, especially titanium, is an important issue in current biomedical research. Regarding titanium, it is also important to ensure a specific protein modification of its surface because here protein binding that is too random can be observed. Specific nanoscale architectures can be applied to overcome this problem. As recently shown, streptavidin can be used as a coupling agent to immobilize biotinylated fibronectin (bFn) on a TiO(X) surface. Because of the conformation of adsorbed biotinylated fibronectin on a streptavidin monolayer, it is possible to adsorb more streptavidin and biotinylated fibronectin layers. On this basis, an alternating protein multilayer c…

Molecular recognition in biotin-streptavidin systems and analogues at the air-water interface

1992

Abstract Specific interaction between biotin and the protein streptavidin in monolayers of synthetic lipids with biotin headgroups has been shown to lead to formation of highly ordered two-dimensional streptavidin crystals. The same behaviour is observed when using desthiobiotin as lipid headgroup which exhibits a significantly lower binding constant compared with biotin (5 × 10 13 M -1 compared with 10 15 M -1 ). This offers the possibility of detaching competetively the 2D crystalline streptavidin layer by addition of free biotin to the aqueous phase. Use of lipoic acid as lipid headgroup ( K a = 7 × 10 7 M −1 ) leads to formation of small snisotropic protein domains indicating a crystall…

Molecular Recognition of Biotinyl Hydrophobic Helical Peptides with Streptavidin at the Air/Water Interface

1994

Bilayer Formation of Streptavidin Bridged by Bis(biotinyl) Peptide at the Air/Water Interface

1994

Nonlocal Elastic-Damage Interface Mechanical Model

2007

The paper presents a nonlocal extension of the elastic-damage interface mechanical model, which is able to describe the effects of the spatially extended microstructure on the decohesion (or fracture) process along a surface. The key feature of the proposed model is an integral constitutive relation between tractions and displacement jumps at the interface. The presence of an integral kernel brings in the model an internal length measure, which characterizes the transition from the microscale, dominated by heterogeneities and discontinuous media, to the mesoscale, characterized as an enhanced homogenized continuum with nonlocal features. The motivations and the fields of applications of the…

Heterogeneous Database Browsing in WWW Based on Meta Model of Data Sources

2001

This paper describes a development principle and technique for a simple universal multiple database browser. The browser operates by getting information from metamodel of data sources and actual data from legacy data sources. Every element such as entity, field, and relation is mapped to some component of HTML page with appropriate structure and layout. Many templates of information layouts can be created allowing to dynamical changing of HTML page to acceptable user interface. The wrappers are used to provide browser with actual data and to act as mediators between data sources and browser. This approach allows to quickly describing new data sources, creating wrappers, making modifications…

A software tool via web for the statistical data analysis; R-php

2007

The spread of Internet and the growing demand of services from the web users have changed and are still changing the way to organize the work or the study. Nowadays, the main part of information and many services are on the web and the software is going toward the same direction: in fact, the use of software implemented via web is ever-increasing, with a client-server logic that enables the “centralized” use of software installed on a server. In this paper we describe the Structure and the running of R-php, an environment for statistical analysis, freely accessible and attainable through the World Wide Web, based on the statistical environment R. R-php is based on two modules: a base module…

Two-dimensional filters for structured text

1997

The paper introduces a method for defining filters for structured text. In the method, the text structure is originally defined by a grammar consisting of a set of productions. To describe the information interests, a two-dimensional template is first created interactively from the grammar to show the structure of a set of textual elements, at a chosen level of detail. The template depicts the hierarchical structure of the elements and indicates also optionality, alternatives, and iteration in the structure. Then, the template is filled by constraints and annotations. The constraints allow giving conditions to the content of parts, to the position of parts in an ordered set of parts, and to…