Search results for "INTERFERENCE"
showing 10 items of 573 documents
Live cell imaging of duplex siRNA intracellular trafficking.
2015
Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach allowed the observation of a number of hitherto uncharacterized intracellular distribution and degradation processes, commencing with a burst of endosomal releases, followed, in some cases, by fast siRNA influx into the nucleus. The continued observation of intact siRNA against a background of free fluorophores resulting from advanced degradation was possible by a specifically developed imaging …
Endothelial transcriptomic changes induced by oxidized low density lipoprotein disclose an up-regulation of Jak-Stat pathway.
2015
Oxidized low density lipoproteins (oxLDLs) act as an etiological factor in the development of atherosclerosis by modifying the biological properties of endothelial cells through mechanisms of vascular inflammation. To deepen the oxLDL changes at cellular level, a transcriptomic analysis of human umbilical artery endothelial cells (HUAECs) treated with oxLDL was performed to identify the modified signaling pathways. Total RNA was isolated from HUAECs treated with oxLDL (100 μg/ml). Gene expression analysis was carried out using Affymetrix oligonucleotide microarrays. Biological pathway analysis was performed using Ingenuity Pathway Analysis software. Microarray assay demonstrated that oxLDL …
New Calibration Model: Combining Integrated Calibration Method and H-point Standard Addition Method to Detect and Avoid Interference Effects
2018
A new calibration methodology based on the combination of integrated calibration method (ICM) and the H-point standard addition method (HPSAM) is presented. It allows the diagnosis and correction o...
On-line vapor-phase generation combined with Fourier transform infrared spectrometry
2008
We present a critical review of on-line vapor-phase generation combined with Fourier transform infrared spectrometry for detection. We discuss different experimental approaches developed for generating the vapor phase based on different principles (e.g., thermal volatilization, acid-base, redox reaction and covalent hydride generation). Using examples of applications, we try to demonstrate the analytical potential of on-line vapor-phase generation in vibrational spectrometry in order to improve the selectivity and the sensitivity of the measurement step and to make environmentally-friendly procedures available for problem solving in the analytical laboratory. We pay special attention to int…
Ultra-high-pressure liquid chromatography tandem mass spectrometry method for the determination of 9 organophosphate flame retardants in water samples
2016
Few methods are available for comprehensive organophosphate flame retardants (PFRs) detection in water and wastewater. Gas chromatography has been employed previously, but this approach is less selective, not amenable for use with deuterated standards and can suffer unfavorable fragmentation. Ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-QqQ-MS/MS) has become the most promising platform, already applied successfully for analysis of selected PFRs in some environmental matrices like water and wastewater. However, the presence of some interferences from the dissolvent, the equipment and the used materials should be taken into account. The procedure involves: The fir…
Transcriptome profiling of citrus fruit response to huanglongbing disease.
2010
Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of phot…
Data for: Secondary Users Selection and Sparse Narrow-band Interference Mitigation in Cognitive Radio Networks
2018
Spectrum scarcity is a critical problem that may reduce the effectiveness of wireless technologies and services. To address this problem, different spectrum management techniques have been proposed such as overlay cognitive radio (CR) where the unlicensed users can share the same spectrum with the licensed users. The main challenges in overlay CR networks are the identification and detection of the Primary User (PU) signals in a multi-source narrow-band interference (NBI) scenario. Therefore, in this paper, we investigate the performance of an orthogonal frequency division multiplexing (OFDM) overlay CR network with Secondary Users (SUs) and subcarriers selection schemes. Three approaches f…
Best not to bet on the horserace: A comment on Forrin and MacLeod (2017) and a relevant stimulus-response compatibility view of colour-word contingen…
2018
International audience; One powerfully robust method for the study of human contingency learning is the colour-word contingency learning paradigm. In this task, participants respond to the print colour of neutral words, each of which is presented most often in one colour. The contingencies between words and colours are learned, as indicated by faster and more accurate responses when words are presented in their expected colour relative to an unexpected colour. In a recent report, Forrin and MacLeod (2017b, Memory & Cognition) asked to what extent this performance (i.e., response time) measure of learning might depend on the relative speed of processing of the word and the colour. With keypr…
An Improved Detection Technique for Cyclic-Prefixed OFDM
2010
A novel Orthogonal Frequency Division Multiplexing detection technique compatible to standard (e.g. Wireless LAN) transmitters is proposed. It features enhanced error-rate performance with flexible computational complexity and robustness to imperfect channel estimation. It is based on exploitation of the redundancy available in the cyclic prefix after cancellation of interference from the preceding block. In order to show the effectiveness of our proposal, an analysis of computational complexity and a number of comparisons to the standard per-subcarrier receiver and a previously existing method in terms of error rates are reported.
Resource allocation for clustered network MIMO OFDMA systems
2012
In this article, we address the resource allocation problem for the downlink of a large network multiple input multiple output orthogonal frequency division multiplexing system with 3-sector base stations. The system is statically divided into a number of disjoint clusters of sectors. A two-step resource allocation scheme is proposed involving the inter-cluster and the intra-cluster levels. As a first step or inter-cluster level, two cooperative frequency reuse approaches are designed to mitigate the inter-cluster interference. A user partition method is proposed to divide the users of each cluster into cluster-edge and cluster-center users. To balance the cell-edge and the cell-average per…