Search results for "INVESTIGATION"

showing 10 items of 366 documents

Apis mellifera sicula Montagano Polymorphism investigation by molecular methods.

2006

Settore AGR/11 - Entomologia Generale E Applicatamolecular investigation Apis m. sicula.
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Il caglio in pasta utilizzato per la produzione di formaggi tradizionali siciliani: indagine microbiologica

2014

Settore AGR/19 - Zootecnica Specialetraditional sicilian cheeses microbial investigationrennet paste
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Vastedda della valle del Belìce cheese: production and microbiological investigation

2007

Settore AGR/19 - Zootecnica Specialevastedda cheese microbiological investigation
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DNA analysis as tool for identification of bacteria in archaeological watellogged wood

2014

S32. Wooden artifacts: from wood identification to dating and conservation/restoration strategies DNA analysis as tool for identification of bacteria in archaeological waterlogged wood Palla, Franco; University of Palermo; Palermo; Italy Barresi, Giovanna; University of Palermo; Palermo; Italy Di Carlo, Enza; University of Palermo; Palermo; Italy In this work molecular techniques were applied in order to integrate the results obtained by Optical (OM) and Scanning Electron (SEM) Microscopy, to understanding and assessing the changes in the anatomical structure of archaeological waterlogged wood (Pinus sp.) induced by bacteria colonization. Observation of wooden thin sections by OM showed the…

Settore BIO/03 - Botanica Ambientale E Applicata16S rDNA ITS rDNA SEM molecula investigation
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Plant products as biocides for conservation of cultural asset sustainable for human and environmental health

2022

In the last decades plants products are increasingly used also in defining innovative procedures for sustainable conservation of cultural heritage. Particularly, essential oils or hydro-alcoholic extracts have been fruitful applied to contrast microbial colonization on organic and inorganic artworks or insect infestation (Anobidae) having regard to the repellent action.
 In this paper, extracts from Asteraceae and Lamiaceae plant families are utilized to counteract widespread microbial colonization (bacteria, cyanobacteria, fungi) due to their antimicrobial activity. In order to define the adequate concentration correlating it to microbial species detected, the antimicrobial activity o…

Settore BIO/03 - Botanica Ambientale E ApplicataGeneral EngineeringGeneral Earth and Planetary SciencesBiodeterioration plant extract green biocides molecular investigation cultural heritage.General Environmental Science
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Bacteria consortia and deterioration of archaeological waterlogged wood: identification by molecular and microscopy techniques

2014

In this study molecular tools are applied to reveal and identify bacterial colonization in waterlogged wood to assessing the changes induced in anatomical structure, previously observed by Optical and Scanning Electron Microscopy (1). The results obtained by observation of wooden thin sections (OM), shown the presence of black and dark-brown areas and mineral concretions. The SEM analysis revealed a specific cell walls alteration, attributable to bacterial activity, other than abundant pyrite framboids (FeS2). The presence of sulfur compounds in archaeological waterlogged wood can indicate both long-term burial in anoxic environment and colonization by sulfate-reducing bacteria. Molecular m…

Settore BIO/03 - Botanica Ambientale E ApplicataWaterlogged wood Biodeterioration Molecular investigation
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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Ionic Liquids: as the different structural order degree can affect the outcome of a reaction

Settore CHIM/06 - Chimica Organicaionic liquids MRH NMR investigation
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Diagnostic Multidisciplinary Investigations for Cultural Heritage at Etna Volcano: A Case Study from the 1669 Eruption in the Mother Church at the Ol…

2022

Misterbianco is located on the southern flank of Mt. Etna (Unesco site), in eastern Sicily (Italy). This site, also known as Monasterium Album, has a long and tormented history linked with volcanic activity of Mt. Etna and regional seismicity. This site received much attention in the 2000s when excavation works brought to light a 14th century church remains below the thick layer of the 1669 lava. This study documents the first diagnostic multidisciplinary survey performed at this site 350 years after the eruption: the investigations were performed by using techniques such as ground-penetrating radar, infrared thermography, a terrestrial laser scanner and a drone survey to analyze the site’s…

Settore GEO/11 - Geofisica Applicata1669 eruption cultural heritage Etna GPR IRT multidisciplinary investigation old Misterbianco TLS UASGeneral Earth and Planetary Sciencesmultidisciplinary investigation; GPR; IRT; TLS; UAS; cultural heritage; old Misterbianco; Etna; 1669 eruption
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Microstructure and mechanical behaviour of a saturated compacted scaly clay

2011

The paper presents the results of a research on the influence of structure characteristics on the mechanical behaviour of a saturated compacted scaly clay. To this aim, mechanical properties of compacted scaly clay samples after saturation are compared with the corresponding properties measured on the clay reconstituted in the laboratory. For the stress range explored in the experimental programme, compacted scaly clay samples are stiffer and stronger than reconstituted clay samples owing to their greater density. Collected results are discussed taking in consideration microstructure characteristics of the compacted and reconstituted clay samples

Settore ICAR/07 - Geotecnicascaly clay experimental investigation microstructure mechanical behaviour
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