Search results for "ISIA"
showing 10 items of 1032 documents
Polyclonal antibodies to mannan from yeast also recognize the carbohydrate structure of gp120 of the AIDS virus: an approach to raise neutralizing an…
1990
This study initiates a new method of developing an antigen which might be useful in the prevention of HIV-1 infection. Using a mannan preparation from Saccharomyces cerevisiae neutralizing antiserum was raised in rabbits which prevents HIV-1 infection in vitro up to a titre of 1:128. The corresponding antibody preparation neutralized the in vitro infectivity down to a concentration of 5 micrograms/ml. Analytical studies suggest that the antibodies are directed against the mannose residues of the HIV-1 glycoprotein (gp) 120 and its precursor gp 160.
General biological properties of antioxidant permeatoxins and their influence on natural and model membranes
1994
Antioxidant defense parameters as predictive biomarkers for fermentative capacity of active dried wine yeast.
2014
The production of active dried yeast (ADY) is a common practice in industry for the maintenance of yeast starters and as a means of long term storage. The process, however, causes multiple cell injuries, with oxidative damage being one of the most important stresses. Consequentially, dehydration tolerance is a highly appreciated property in yeast for ADY production. In this study we analyzed the cellular redox environment in three Saccharomyces cerevisiae wine strains, which show markedly different fermentative capacities after dehydration. To measure/quantify the effect of dehydration on the S. cerevisiae strains, we used: (i) fluorescent probes; (ii) antioxidant enzyme activities; (ii) in…
Use of Saccharomyces cerevisiae and Caenorhabditis elegans as model organisms to study the effect of cocoa polyphenols in the resistance to oxidative…
2011
Developing functional foods to improve the quality of life for elderly people has great economic and social impact. Searching for and validating ingredients with in vivo antioxidant effects is one of the key steps in developing this kind of food. Here we describe the combined use of simple biological models and transcriptomics to define the functional intracellular molecular targets of a polyphenol-enriched cocoa powder. Cocoa powder supplemented culture medium led to increased resistance to oxidative stress, in both the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans, and, in the latter, lifespan was also increased. These effects are fully dependent on the po…
Proteomic Analysis of Saccharomyces cerevisiae Response to Oxidative Stress Mediated by Cocoa Polyphenols Extract
2020
The present study addressed the protective effects against oxidative stress (OS) of a cocoa powder extract (CPEX) on the protein expression profile of S. cerevisiae. A proteomic analysis was performed after culture preincubation with CPEX either without stress (&minus
The role of post-transcriptional modulators of metalloproteins in response to metal deficiencies
2021
Copper and iron proteins play a wide range of functions in living organisms. Metal assembly into metalloproteins is a complex process, where mismetalation is detrimental and energy-consuming to cells. Under metal deficiency, metal distribution is expected to reach a metalation ranking, prioritizing essential versus dispensable metalloproteins, while avoiding interferences with other metals and protecting metal-sensitive processes. In this review, we propose that posttranscriptional Modulators of Metalloprotein messenger RNA (ModMeR) are good candidates in metal prioritization under metal-limited conditions. ModMeR target high quota or redundant metalloproteins and, by adjusting their synthe…
Herbicidal potential of aqueous extracts from Melia azedarach L., Artemisia arborescens L., Rhus coriaria L. and Lantana camara L.
2017
In the search for new strategies for weed management in agricultural systems, a great interest is to use the plant extracts to replace or integrate, chemical weed control. Two experiments were done to test the effects of plant water extracts from Chinaberry (Melia azedarach L.), Tree Wormwood (Artemisia arborescens (Vaill.) L.), Sicilian Sumac (Rhus coriaria L.) and Lantana (Lantana camara L.) on seed germination of Rocket (Eruca sativa Mill.), Rapeseed (Brassica napus L.), Bladderflower (Araujia sericifera Brot.) and Psyllium (Plantago psyllium L). The water extracts (pure and 50% mixtures) from the donor species were applied on seeds of recipient plants. In second experiment in pots, thes…
Architectures des Italiens à l'époque du Protectorat francais
2011
Il contributo degli italiani al “nuovo” volto della Tunisia nel periodo del protettorato francese ha la sua più tangibile manifestazione nel settore della produzione edilizia. È principalmente nella capitale Tunisi che, fino all’inizio degli anni Quaranta del XX secolo, si manifesta con maggiore incisività e riconoscibilità questo fenomeno nel contesto della considerevole trasformazione urbana operata, a partire dall’ultimo decennio dell’Ottocento, con l’attuazione del piano di ampliamento redatto dai tecnici del Genio Militare dell’Esercito Francese. Inizia così la mutazione d’età contemporanea della forma urbana di Tunisi. Diversamente da altri impianti urbani realizzati sempre dall’ammin…
Effect of nitrogen limitation and nature of the feed upon Oenococcus oeni metabolism and extracellular protein production
2005
Aims: The aim of the study was to characterize the effect of various nitrogen sources on Oenococcus oeni growth, carbon source utilization, extracellular protease activity and extracellular proteins. More generally, the goal is to understand how nitrogen-based additives might act to enhance malolactic fermentation in wine. Methods and Results: Five yeast extracts were used. As the amino acid and nitrogen analyses revealed, they were similar in global amino acid composition, except for arginine level. Nevertheless the ratio of amino acids between free/bound, and low/high molecular weight fractions were highly different. One of the yeast extracts led to a significant protease activity in th…