Search results for "InSe"
showing 10 items of 3069 documents
The onset of metamorphosis in Tenebrio molitor larvae (Insecta, Coleoptera) under grouped, isolated and starved conditions
1991
Abstract The post-embryonic development of the beetle Tenebrio molitor presents a variable number of larval instars. Several parameters (instar length, time of apolysis and cuticle deposition) were compared during the larval-larval and larval-pupal cycles of mealworms over 50 mg, reared in grouped or isolated conditions. In grouped conditions comparable to mass breeding, larval-larval and larval-pupal apolyses were found to occur at the same time, but instar duration was longer in the case of prepupae. However, isolation was found to accelerate larval-pupal (but not larval-larval) apolyses and to reduce the number of larval instars, whereas starvation inhibited larval-larval (but not larval…
Evolution of ecdysteroids and of their apolar conjugates during the post-embryonic development of the tickOrnithodoros moubata
1997
The ecdysteroid (ES) content of the soft tick Ornithodoros moubata was investigated during the five successive nymphal molting cycles by means of an enzyme immunoassay (EIA). Samples were submitted to esterase hydrolysis in order to release free ecdysteroids from the acyl-ester conjugates (AP = apolar products). Crude and hydrolysed extracts were then analyzed by EIA using two different antibodies, a monoclonal raised against 20-hydroxyecdysone (20E) and a polychlonal raised against ecdysone (E). With the crude extracts, each molting cycle was associated with an ES peak, occurring in the middle of the instar. 20E was preponderant during the first 2 nymph cycles, but the proportion of E and …
Ecdysteroid titres in a tenebrionid beetle, Zophobas atratus: effects of grouping and isolation.
1997
Metamorphosis in Zophobas atratus is dependent on isolation: when kept in grouped conditions, larvae undergo numerous supernumerary moults, growing in size, without pupating. This beetle thus represents an interesting model for the analysis of possible differences in the endocrine regulation of normal vs. supernumerary larval moults. In this study, the ecdysteroid titres have been analysed in this species, using enzyme immunoassay. The hormonal variations of larvae undergoing normal or supernumerary larval cycles were particularly examined, in either grouped or isolated conditions. Normal larval cycles presented very similar ecdysteroid variations in grouped as well as isolated conditions, …
Slow Infection due to Lowering the Amount of Intact versus Empty Particles Is a Characteristic Feature of Coxsackievirus B5 Dictated by the Structura…
2019
Enterovirus B species typically cause a rapid cytolytic infection leading to efficient release of progeny viruses. However, they are also capable of persistent infections in tissues, which are suggested to contribute to severe chronic states such as myocardial inflammation and type 1 diabetes. In order to understand the factors contributing to differential infection strategies, we constructed a chimera by combining the capsid proteins from fast-cytolysis-causing echovirus 1 (EV1) with nonstructural proteins from coxsackievirus B5 (CVB5), which shows persistent infection in RD cells. The results showed that the chimera behaved similarly to parental EV1, leading to efficient cytolysis in both…
Coxsackievirus A9 Infects Cells via Nonacidic Multivesicular Bodies
2014
ABSTRACT Coxsackievirus A9 (CVA9) is a member of the human enterovirus B species in the Enterovirus genus of the family Picornaviridae . According to earlier studies, CVA9 binds to αVβ3 and αVβ6 integrins on the cell surface and utilizes β2-microglobulin, dynamin, and Arf6 for internalization. However, the structures utilized by the virus for internalization and uncoating are less well understood. We show here, based on electron microscopy, that CVA9 is found in multivesicular structures 2 h postinfection (p.i.). A neutral red labeling assay revealed that uncoating occurs mainly around 2 h p.i., while double-stranded RNA is found in the cytoplasm after 3 h p.i. The biogenesis of multivesicu…
Early entry events in Echovirus 30 infection
2020
Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, surprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 strain Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for successful infection, but not the coxsackievirus and adenovirus receptor (CAR) or decay-accelerating factor (DAF), although an interaction with …
Les Diplômés DNAT 2005 : Enquête nationale sur le parcours d'insertion professionnelle 18 mois après le diplôme
2007
Le diplôme national d'arts et techniques (DNAT) conclut le cycle court (3 ans) des écoles supérieures d'art françaises. Cette étude présente les résultats d'une enquête nationale sur les diplômés de la promotion 2005. Les conditions d'insertion de ces diplômés sont observées et analysées 18 mois après le diplôme. La prochaine enquête sur cette promotion interviendra à l'échéance de 36 mois. (Rapport fin 2008)
Dinner with the roommates: trophic niche differentiation and competition in a mutualistic ant‐ant association
2020
1. The potential for competition is highest among species in close association. Despite net benefits for both parties, mutualisms can involve costs, including food competition. This might be true for the two neotropical ants Camponotus femoratus and Crematogaster levior, which share the same nest in a presumably mutualistic association (parabiosis). 2. While each nest involves one Crematogaster and one Camponotus partner, both taxa were recently found to comprise two cryptic species that show no partner preferences and seem ecologically similar. Since these cryptic species often occur in close sympatry, they might need to partition their niches to avoid competitive exclusion. 3. Here, we in…
Different trophic positions among social vespid species revealed by stable isotopes
2021
The social vespid wasps are common insect predators and several species behave in unison in the same biotopes. It is commonly accepted that social wasps are mainly opportunistic generalist predators without differences in prey selection and hence they compete for the same food resources. Trophic positions of six vespid wasp species and their potential prey from four sites in Finland and one in the UK were evaluated using carbon and nitrogen stable isotopes (δ13C and δ15N). The difference in isotope values indicated different trophic positions among species. In general, Dolichovespula spp. showed higher δ15N values than Vespula spp., which suggests that Dolichovespula forage on higher trophi…
Soil decomposer animal community in heavy-metal contaminated coniferous forest with and without liming
2002
Abstract Responses of decomposer animals to heavy-metal contamination were studied near a Cu–Ni smelter in Finland. Samples were taken 0.5, 2 and 8 km from the smelter. In addition, plots fertilised with lime were sampled. Decomposer community in coniferous forest soil appeared to be quite resistant to heavy-metals. Only in the vicinity (0.5 km) of the smelter, were numbers of soil animals clearly decreased and their community structure strongly altered as compared to the control site (8 km). At the 2-km site, the community structure was only slightly changed. Most of the collembolan species were still found at the 0.5-km site. High metal sorption capacity of the humus, and heterogeneous di…