Search results for "InSe"
showing 10 items of 3069 documents
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymati…
1997
The NS5B protein of the hepatitis C virus (HCV) is an RNA-dependent RNA polymerase (RdRp) (S.-E. Behrens, L. Tomei, and R. De Francesco, EMBO J. 15:12-22, 1996) that is assumed to be required for replication of the viral genome. To further study the biochemical and structural properties of this enzyme, an NS5B-hexahistidine fusion protein was expressed with recombinant baculoviruses in insect cells and purified to near homogeneity. The enzyme was found to have a primer-dependent RdRp activity that was able to copy a complete in vitro-transcribed HCV genome in the absence of additional viral or cellular factors. Filter binding assays and competition experiments showed that the purified enzym…
Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry
2012
ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…
Papillomavirus assembly requires trimerization of the major capsid protein by disulfides between two highly conserved cysteines.
1998
ABSTRACT We have used viruslike particles (VLPs) of human papillomaviruses to study the structure and assembly of the viral capsid. We demonstrate that mutation of either of two highly conserved cysteines of the major capsid protein L1 to serine completely prevents the assembly of VLPs but not of capsomers, whereas mutation of all other cysteines leaves VLP assembly unaffected. These two cysteines form intercapsomeric disulfides yielding an L1 trimer. Trimerization comprises about half of the L1 molecules in VLPs but all L1 molecules in complete virions. We suggest that trimerization of L1 is indispensable for the stabilization of intercapsomeric contacts in papillomavirus capsids.
Genome sequence of SeIV-1, a novel virus from the Iflaviridae family infective to Spodoptera exigua
2011
Analysis of the transcriptome of Spodoptera exigua larvae revealed the presence of several ESTs with homology to virus of the order Picornavirales and with the highest similarity to Infectious flacherie virus (Iflaviridae) that infects Bombyx mori larvae. Iflaviridae is a recently defined family of insect-infecting viruses that consist of positive single strand RNA genomes translated into a single polyprotein of around 3000 amino acids long. Using the sequence information derived from the obtained ESTs, we have completed the genomic sequence of this virus. The novel S. exigua iflavirus (SeIV-1) has a genome of 10.3 kb and codes for a 3222 aa polyprotein. Expression analysis has revealed the…
Human Papillomavirus Type 16 E7 Peptide-Directed CD8+ T Cells from Patients with Cervical Cancer Are Cross-Reactive with the Coronavirus NS2 Protein
2003
ABSTRACTHuman papillomavirus type 16 (HPV16) E6 and E7 oncoproteins are required for cellular transformation and represent candidate targets for HPV-specific and major histocompatibility complex class I-restricted CD8+-T-cell responses in patients with cervical cancer. Recent evidence suggests that cross-reactivity represents the inherent nature of the T-cell repertoire. We identified HLA-A2 binding HPV16 E7 variant peptides from human, bacterial, or viral origin which are able to drive CD8+-T-cell responses directed against wild-type HPV16 E7 amino acid 11 to 19/20 (E711-19/20) epitope YMLDLQPET(T) in vitro. CD8+T cells reacting to the HLA-A2-presented peptide from HPV16 E711-19(20)recogni…
SARS-CoV-2 envelope protein topology in eukaryotic membranes
2020
Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…
“Voz del alumnado”: hacer de los estudiantes participantes activos y responsables en los procesos formativos activados en la escuela y en la universi…
2017
"Voce degli studenti" è un movimento pedagogico nato negli anni novanta in alcuni paesi di lingua inglese (Stati Uniti, Canada, Inghilterra e Australia). Supporta la partecipazione attiva e responsabile degli studenti ai processi di formazione attivati nella scuola e nell'università. Per includere le voci degli studenti in questi processi, gli adulti (insegnanti e amministratori) devono riconoscere i loro diritti di essere ascoltati e rispettati come persone con interessi, bisogni di apprendimento e idee che possono risolvere i problemi della didattica scolastica/universitaria, della ricerca educativa e delle politiche culturali in cui si devono prendere decisioni che li riguardano. In ques…
Trabalho inclusivo e digno de pessoas em situação de vulnerabilidade: das políticas às metodologias de acompanhamento em organizações da economia soc…
2022
En un contexto de transición justa e inclusiva, este estudio analiza el ámbito de la inserción sociolaboral de las personas en situación de vulnerabilidad, a partir de las políticas que lo configuran y de las metodologías de seguimiento en las organizaciones de la economía social (OES). Aunque el derecho al trabajo está consagrado legalmente como un derecho universal, para una amplia gama de grupos clasificados como vulnerables, desfavorecidos o en situación de desventaja, este derecho no está garantizado. Esta investigación se centra en las OES tanto por su idoneidad en cuanto a la finalidad y los principios que guían su funcionamiento como por su tradición e importancia en cuanto a la int…
Šinsengumi militārās vienības loma Japānas vēsturē un kultūrā
2018
Bakalaura darbā “Šinsengumi militārās vienības loma Japānas vēsturē un kultūrā” tiek atspoguļota Šinsengumi militārās vienības loma Edo perioda norieta posmā un šī tēla izmantošana mūsdienu populārajā kultūrā. Konkrēti tiek pētīta vienības vēstures gaita un tās tēla ietekme uz populāro kultūru. Tēma tika izvēlēta, pamatojoties uz to, ka pēdējo desmit gadu laikā Šinsengumi tēls pasaulē izplatās vienlaikus Japānas populārās kultūras produktu popularitātes uzplaukumam. Populārās kultūras veidotais tēls tiek romantizēts un realitāte nereti pārklājas ar izdomu, taču interesentiem nav iespēju vērtēt atveidotās vēstures patiesumu, jo pastāv autoritatīvu avotu trūkums jebkādā no valodām, izņemot ja…
Seeing red? Colour biases of foraging birds are context dependent.
2020
Funder: Suomen Kulttuurirahasto; doi: http://dx.doi.org/10.13039/501100003125