Search results for "InSe"
showing 10 items of 3069 documents
Response and Recovery of Brain Acetylcholinesterase Activity in the European Eel,Anguilla anguilla,Exposed to Fenitrothion
1998
European eel (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. Brain acetylcholinesterase (AChE) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h pesticide exposure. Results indicated that AChE activity in eel brains decreased as the concentration of fenitrothion increased. The pesticide induced significant inhibitory effects on the AChE activity ofA. anguilla,ranging from >40% inhibition at a sublethal concentration of 0.02 ppm to >60% inhibition at a sublethal concentration of 0.04 ppm. Eel were exposed to both fenitrothion concentrations for 96 h and then allowed a period of recovery in pesticide-f…
A pheromone-binding protein from the cockroach Leucophaea maderae: cloning, expression and pheromone binding
2003
0264-6021 (Print) Journal Article; Odorant-binding proteins (OBPs) are thought to transport volatile compounds from air to their receptors through the sensillary lymph. In this protein family, the subgroup of pheromone-binding proteins (PBPs) is specifically tuned to the perception of the sexual pheromone. To date, the description of OBPs has been restricted to Endopterygota and Paraneoptera. Their expression in Orthopteroid has been hypothesized, but no evidence of OBP has been produced in this assemblage to date. In the present study, we describe the first OBP from a Dictyopteran insect that belongs to the cockroach Leucophaea maderae. The PBP of L. maderae (PBPLma) shares all the hallmar…
Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi.
2016
Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters…
Identification and characterisation of the dopamine receptor II from the cat flea Ctenocephalides felis (CfDopRII)
2006
International audience; G protein-coupled receptors (GPCRs) represent a protein family with a wide range of functions. Approximately 30% of human drug targets are GPCRs, illustrating their pharmaceutical relevance. In contrast, the knowledge about invertebrate GPCRs is limited and is mainly restricted to model organisms like Drosophila melanogaster and Caenorhabditis elegans. Especially in ectoparasites like ticks and fleas, only few GPCRs are characterised. From the cat flea Ctenocephalides felis, a relevant parasite of cats and dogs, no GPCRs are known so far. Thus, we performed a bioinformatic analysis of available insect GPCR sequences from the honeybee Apis mellifera, the mosquito Anop…
Near-infrared imaging and structured light ranging for automatic catheter insertion
2006
Vein localization and catheter insertion constitute the first and perhaps most important phase of many medical procedures. Currently, catheterization is performed manually by trained personnel. This process can prove problematic, however, depending upon various physiological factors of the patient. We present in this paper initial work for localizing surface veins via near-infrared (NIR) imaging and structured light ranging. The eventual goal of the system is to serve as the guidance for a fully automatic (i.e., robotic) catheterization device. Our proposed system is based upon near-infrared (NIR) imaging, which has previously been shown effective in enhancing the visibility of surface vein…
Bacillus thuringiensis Cry1Ac Toxin-Binding and Pore-Forming Activity in Brush Border Membrane Vesicles Prepared from Anterior and Posterior Midgut R…
2008
ABSTRACT It is generally accepted that Bacillus thuringiensis Cry toxins insert into the apical membrane of the larval midgut after binding to specific receptors, and there is evidence that the distribution of binding molecules along the midgut is not uniform. By use of the voltage-sensitive dye DiSC 3 (5) and 125 I-labeled Cry1Ac, we have measured the effect of Cry1Ac in terms of permeabilization capacity and of binding parameters on brush border membrane vesicles (BBMV) prepared from the anterior and the posterior regions of the larval midgut from two insect species, Manduca sexta and Helicoverpa armigera . The permeabilizing activity was significantly higher with BBMV from the posterior …
A GFP-tagged Muscleblind C protein isoform reporter construct
2010
Drosophila muscleblind (mbl), the ortholog of human Muscleblind-like 1 (MBNL1) gene involved in Myotonic Dystrophy (DM), gives raise to protein isoforms MblA to G. The specific functions and subcellular distribution of isoforms are still largely unknown. To overcome the lack of isoform-specific antibodies we generated transgenic flies that express a GFP:MblC fusion protein under the control of the Gal4/UAS system. The reporter fusion protein was able to functionally complement mbl loss of function mutations, demonstrating activity, and accumulated predominantly in adult muscle nuclei. The fluorescent nature of the reporter makes it appropriate for live imaging detection of MblC protein isof…
Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering.
2003
We have examined a novel biomaterial consisting of a non-woven fibroin net produced from silk (Bombyx mori) cocoons for its ability to support the growth of human cells. Various human cells of different tissue and cell types (endothelial, epithelial, fibroblast, glial, keratinocyte, osteoblast) were examined for adherence and growth on the nets by confocal laser microscopy after staining of the cells with calcein-AM and by electron microscopy. All the cells readily adhered and spread over the individual fibers of the nets. Most of the cells were able to grow and survive on the nets for at least 7 weeks and growth not only covered the individual fibers of the net but generally bridged the ga…
Mesadenes maturation and its hormonal control in the milkweed bug, Oncopeltus fasciatus
1993
Abstract Adult maturation of the mesadenia was examined by electron microscopy. The glandular epithelium exhibits only one cell type with a polarity along the haemocoel-lumen axis. In newly emerged males, the glands show little signs of secretory activity although the narrow lumen contains some material. Dramatic changes commence at day 2. The rER increases drastically, and at day 3 cisternae of the rER are considerably distended and include a fine granulated product. The lumen of the gland widens greatly and stores material of the same appearance as in the rER inclusions. The mode of the secretory process is not clear since membrane bound vesicles are not formed. SDS-PAGE of the secretion …
Morphology of follicle cells of Libellulidae (Odonata)
2011
In libellulids, mature oocyte size varies within and between individual ovaries. The regulating mechanism is not yet understood. Variations in the contents of the follicle cells, and thereby their ability to secrete material into the oocyte, might explain some of the observed differences in oocyte size. We therefore investigated the follicle cell surface, the interstitial space width between follicle cells and between follicle cells and oocytes, the number of nuclei, and the cell compartment proportions using scanning and transmission electron microscopy. In all investigated species, the follicle cells were covered by a basal lamina. We found cytoplasmic microvilli and septate junctions. As…