Search results for "Inmunosorbent"

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Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected r…

2003

The present study reports on the development of a coproantigen capture enzyme-linked immunosorbent assay (ELISA) for detecting Echinostoma caproni in experimentally infected rats. The capture ELISA was based on polyclonal rabbit antibodies that recognize excretory–secretory (ES) antigens. The detection limit of pure ES was 3 ng/ml in sample buffer and 60 ng/ml in fecal samples. The test was evaluated using a follow-up of 10 rats experimentally infected with 100 metacercariae of E. caproni, and the results were compared with those of other diagnostic methods such as parasitological examination and antibody titers determined by indirect ELISA. Coproantigens were detected in all the infected r…

MaleAntibodies HelminthEchinostoma caproniEnzyme-Linked Immunosorbent Assay:CIENCIAS DE LA VIDA [UNESCO]ExcretionFecesAntigenEchinostomaCoproantigen ExcretionUNESCO::CIENCIAS DE LA VIDAAnimalsRats WistarAntibodyEcology Evolution Behavior and SystematicsFecesEchinostomiasisbiology:CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Parasitología animal [UNESCO]Antibody titerInfected ratsbiology.organism_classificationAntibody ; Echinostoma caproni ; Infected rats ; Coproantigen Excretion ; InmunosorbentVirologyRatsKineticsUNESCO::CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Parasitología animalPolyclonal antibodiesAntigens HelminthImmunologybiology.proteinParasitologyRabbitsAntibodyTrematodaEchinostomaInmunosorbent
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