Search results for "Inoculation"
showing 10 items of 105 documents
Metarhizium anisopliae, a potential agent for the control of grape phylloxera
2004
This investigation deals with the control effects of the insect pathogenic fungus Metarhizium anisopliae var. anisopliae on Daktulosphaira vitifoliae. In pot experiments, the soil surrounding phylloxera-infected grapes was inoculated with barley colonised with M. anisopliae. After thirty-two days, ineight of ten Metarhizium-applied pots nofresh phylloxera infections could be observed.In two of ten plants, a few fresh nodositiessingly occupied with phylloxera or phylloxeraeggs could be found. In all untreated plants,fresh nodosities with either single (two of sixplants) or multiple (four of six plants)occupation with phylloxera could be observed.M. anisopliae could be re-isolated in aconcent…
Supression of Botrytis cinerea causing grey mould disease of grape vine (Vitis vinifera) and its pectinolytic activities by a soil bacterium
1997
Abstract Botrytis cinerea was found to be pathogenic to grapevine, producing characteristic grey mould symptoms within 7 days of inoculation into vitroplants. An endospore forming bacterial strain (GI 070), Bacillus circulans, was found to be antagonist against this disease causing fungus. Under laboratory conditions the bacterial culture or its filtrate brings about complete suppression of the fungus in Petri-dishes and the grey mould symptoms on grapevine vitroplants. Pectinolytic activities (Polygalacaturonase, Pectin lyases) of Botrytis cinerea were also influenced by these bacteria. Details of the fungal parasite, its biological control and its pectinolytic activities are discussed in …
Degradation of stilbene-type phytoalexins in relation to the pathogenicity of Botrytis cinerea to grapevines
1996
The ability of eight isolates of Botrytis cinerea to degrade the stilbene phytoalexins, resveratrol and pterostilbene, was compared with their pathogenicity to grapevines. All strains which degraded resveratrol and pterostilbene were highly or moderately pathogenic to in vitro cultures of grapevines (Vitis rupestris) after inoculation with agar disks containing mycelium, while those which were unable to degrade phytoalexins were non-pathogenic. In all cases, the hydroxystilbene-degrading activity was related to the presence of laccase activity in the culture filtrates, as shown by using syringaldazine as substrate. The role of laccase-mediated degradation of phytoalexins in relation to path…
Yeast interaction on Chardonnay wine composition: Impact of strain and inoculation time.
2022
Abstract It is of great importance to understand the molecular characteristics and substantial chemical transformations due to yeast-yeast interaction. Non-targeted metabolomics was used to unravel must in fermentation composition, inoculated with non-Saccharomyces (NS) yeasts and Saccharomyces cerevisiae (S) for sequential fermentation. ultrahigh-resolution mass spectrometry was able to distinguish thousands of metabolites and provides deep insights into grape must composition allowing better understanding of the yeast-yeast interactome. The dominance of S, characterized by a metabolic richness not found with NS, is dependent on inoculation time and on the yeast species present. Co-inocula…
The effect of arbuscular mycorrhizal fungi on total plant nitrogen uptake and nitrogen recovery from soil organic material
2013
SUMMARYArbuscular mycorrhizal (AM) fungi increase nitrogen (N) uptake by their host plants, but their role in plant N capture from soil organic material is still unclear. In particular, it is not clear if AM fungi compete with the host plant for the N coming from the decomposing organic matter (OM), especially when the AM extraradical mycelium (ERM) and plant roots share the same soil volume. The goal of the present research was to study the effects of AM fungi on wheat N capture after the addition of 15N-labelled OM to soil. Durum wheat (Triticum durum) was grown under controlled conditions in a sand:soil mix and the following treatments were applied: (1) AM inoculation with Glomus mosseae…
Response to salinity stress of Rhizobium leguminosarum bv. viciae strains in the presence of different legume host plants
2011
We investigated the effect of residual salts from the previous summer’s irrigation on two non-irrigated cover crops—broad bean and common vetch—and on their rhizobial symbiontics. Before sowing, seeds were inoculated with a salt-tolerant strain and a salt-sensitive strain of Rhizobium leguminosarum biovar viciae. An increase in the electrical conductivity of the saturated-soil extract from 2.0 dS m−1 to 6.0 dS m−1 caused a severe reduction of broad bean biomass, while growth of common vetch was almost unaffected by the salinity level. Our results clearly indicate that common vetch as a cover crop may increase the availability of nitrogen in soil more than broad bean also in saline environme…
L'inoculation des légumineuses en France : un exemple de coopération entre l'INRA de Dijon et les fabricants d'inoculant
2013
EAMERSslides (31 diapositives); L'inoculation des légumineuses en France : un exemple de coopération entre l'INRA de Dijon et les fabricants d'inoculant
Investigation of the hygienic safety of aromatic plants cultivated in soil contaminated with Listeria monocytogenes
2012
Abstract The present work was undertaken to investigate the survival of Listeria monocytogenes ATCC 19114T in soil during the whole crop cycle of rocket (Eruca sativa Mill.) and basil (Ocimum basilicum L.), to monitor its transfer to the leaves, and to evaluate its viability at harvest. To this purpose, the soil was N–P–K fertilized and four trials, obtained with different combinations of soil treatment, listerial inoculums and seed planting, were followed for each aromatic plant. Soil was weekly investigated for total microbial counts and L. monocytogenes evolution. At the starting time, un-inoculated autoclaved soil showed a limited microbial load (103 CFU g dw−1), while un-inoculated non…
The influence of backslopping on lactic acid bacteria diversity in tarhana fermentation
2020
Tarhana is produced at batch systems in which the microbiota has changed accordingly to the microbial load from ingredients. In order to stabilize the microbiota, the effects of backslopping carried out under different temperature regimes (25 and 30 °C), pH (3.70 and 4.00) and inoculation rates (5, 10 and 15%) on lactic acid bacteria (LAB) diversity were determined in tarhana dough. LAB and Total Aerobic Mesophilic Bacteria (TAMB) numbers increased in all tarhana dough samples subjected to backslopping. Temperature and pH significantly affected the microbiological diversity of tarhana whereas the different inoculation rates did not. Tarhana dough showed complex tarhana microbiota following …
Immunology of dermatophytosis
1983
By causing experimental lesions of dermatophytosis in rabbits inoculated and reinoculated with T. mentagrophytes var. granulosum and T. rubrum a study was carried out of the state of cellular immunity response, during infection, and also of the antigens responsible for the sensitization. Cellular immunity response was detected using the leucocyte migration test (L.M.T.) in the presence of antigenic compounds of the 'Keratinase' of Eleuterio et al.