Search results for "Insect"

Characterization and evolution of two bacteriome-inhabiting symbionts in cixiid planthoppers (Hemiptera: Fulgoromorpha: Pentastirini).

2009

International audience; Like other plant sap-sucking insects, planthoppers within the family Cixiidae (Insecta: Hemiptera: Fulgoromorpha) host a diversified microbiota. We report the identification and first molecular characterization of symbiotic bacteria in cixiid planthoppers (tribe: Pentastirini). Using universal eubacterial primers we first screened the eubacterial 16S rRNA sequences in Pentastiridius leporinus (Linnaeus) with PCR amplification, cloning, and restriction fragment analysis. We identified three main 16S rRNA sequences that corresponded to a Wolbachia bacterium, a plant pathogenic bacterium, and a novel gammaproteobacterial symbiont. A fourth bacterial species affiliated w…

Redescription of the forgotten New Caledonian weevil genus Callistomorphus Perroud, 1865 (Coleoptera, Curculionidae, Eugnomini) with descriptions of …

2019

Callistomorphusis one of the “forgotten” genera of the tribe Eugnomini inhabiting rain forest in New Caledonia. In this paper, the genusCallistomorphusand the type speciesC.farinosusare redescribed. Eight new species,Callistomorphusfundatussp. n.,C.gibbussp. n.,C.malleussp. n.,C.minimussp. n.,C.rutaisp. n.,C.szoltysisp. n.,C.torosussp. n.andC.turbidussp. n., are described, originating from the main island of New Caledonia. Illustrations and SEM photographs of the external morphology and the male and female terminalia are provided, as well as dorsal habitus colour photographs of the adults, a key to the species, a distribution map, and a discussion of the systematic position ofCallistomorphu…

Sex versus parthenogenesis: A transcriptomic approach of photoperiod response in the model aphid Acyrthosiphon pisum (Hemiptera: Aphididae)

2007

International audience; Most aphids develop a cyclic parthenogenesis life-cycle. After several generations of viviparous parthenogenetic females, it follows a single annual generation of sexual individuals, usually in autumn, that mate and lay the sexual eggs. Shortening of photoperiod at the end of the summer is a key factor inducing the sexual response. With the survey here reported we aimed at identifying a collection of candidate genes to participate at some point in the cascade of events that lead to the sexual phenotypes. Following a suppression subtractive hybridization methodology (SSH) on the model aphid Acyrthosiphon pisum, we built and characterised two reciprocal cDNA libraries …

2021

Although insect herbivores are known to evolve resistance to insecticides through multiple genetic mechanisms, resistance in individual species has been assumed to follow the same mechanism. While both mutations in the target site insensitivity and increased amplification are known to contribute to insecticide resistance, little is known about the degree to which geographic populations of the same species differ at the target site in a response to insecticides. We tested structural (e.g., mutation profiles) and regulatory (e.g., the gene expression of Ldace1 and Ldace2, AChE activity) differences between two populations (Vermont, USA and Belchow, Poland) of the Colorado potato beetle, Lepti…

The Spodoptera exigua ABCC2 Acts as a Cry1A Receptor Independently of its Nucleotide Binding Domain II

2019

ABC proteins are primary-active transporters that require the binding and hydrolysis of ATP to transport substrates across the membrane. Since the first report of an ABCC2 transporter as receptor of Cry1A toxins, the number of ABC transporters known to be involved in the mode of action of Cry toxins has increased. In Spodoptera exigua, a mutation in the SeABCC2 gene is described as genetically linked to resistance to the Bt-product XentariTM. This mutation affects an intracellular domain involved in ATP binding, but not the extracellular loops. We analyzed whether this mutation affects the role of the SeABCC2 as a functional receptor to Cry1A toxins. The results show that Sf21 cells express…

Revision of the Afrotropical genus Leiodontocercus (Orthoptera, Tettigoniidae, Phaneropterinae) with a description of four new species

2020

Specimens belonging to the genus Leiodontocercus are rare or even absent in natural history museum collections; this is likely due to at least two reasons, notably, their relatively small size, and, the sheer difficulty in finding them in dense Afrotropical forests. Until recently, three species from less than fifteen specimens were known from this genus, whose identification relied on a singular diagnostic character, that is, the shape of the male cerci. The present contribution is based on the examination of thirty specimens collected from various countries, ranging from central to west Africa; apart from the male cerci, a second diagnostic character – the stridulatory file – is used to d…

Palaearctic species of Charipinae (Hymenoptera, Figitidae): two new species, synthesis and identification key

2018

The Charipinae Dalla Torre & Kieffer, 1910 present in the Palaearctic region are revised; 2410 specimens have been identified, belonging to 75 species: 52 to Alloxysta, one to Apocharips, six to Dilyta and 16 to Phaenoglyphis. For 33 species, new country-level distribution records are provided. Two new species are here described: Alloxysta palearctica Ferrer-Suay & Pujade-Villar sp. nov. and Alloxysta pascuali Ferrer-Suay sp. nov. A diagnosis for these species is included and their diagnostic features are shown in different figures. A key to identify all the species of Charipinae in the Palaearctic region is also given.

Charipinae (Hymenoptera: Cynipoidea: Figitidae) present in the Museum of Natural History of the University of Wroclaw, with an identification key for…

2020

Charipinae (Cynipoidea: Figitidae) deposited in the Museum of Natural History of Wroclaw University (Poland) have been studied. Seven species are recorded for the first time from Poland: Alloxysta brachyptera (Hartig, 1840), A. castanea (Hartig, 1841), A. citripes (Thomson, 1862), A. consobrina (Zetterstedt, 1838), A. mullensis (Cameron, 1883), A. nottoni Ferrer-Suay & Pujade-Villar, 2015 and Phaenoglyphis heterocera (Hartig, 1841). The presence of previously recorded species is confirmed and new records are specified. A key to all Charipinae species ever recorded in Poland is given.

New Charipinae ( Hymenoptera : Cynipoidea : Figitidae ) records from China

2016

Abstract Charipinae material collected from different provinces of China has been studied. Twenty-one previously described species have been identified: Alloxysta arcuata, A. brevis , A. carinata , A. castanea , A. consobrina , A. fracticornis , A. leunisii , A. macrophadna , A. mullensis , A. obscurata , A. paretasmartinezi , A. pilipennis , A. pilosa , A. postica , A. sawoniewiczi , A. victrix , A. xanthopa , Dilyta subclavata , Phaenoglyphis chinensis , P. heterocera and P. villosa . All species, except for P. chinensis and P. villosa , are here recorded for the first time from China. Diagnosis, material studied and distribution are given for each species. Plates with the diagnostic morp…

Establishing laboratory cultures and performing ecological and evolutionary experiments with the emerging model speciesChironomus riparius

2019

Chironomus riparius is a well‐established model organism in various fields such as ecotoxicology and ecology, and therefore, environmental preferences, ecological interactions and metabolic traits are well‐studied. With the recent publication of a high‐quality draft genome, as well as different population genetic parameters such as mutation and recombination rate, the species can be used as an alternative to the Drosophila models in experimental population genomics or molecular ecology. To facilitate access to this promising experimental model species for a wider range of researchers, we describe experimental methods to first create and sustain long‐term cultures of C. riparius and then use…