Search results for "Integration"
showing 10 items of 1465 documents
Epigenetic Status of an Adenovirus Type 12 Transgenome upon Long-Term Cultivation in Hamster Cells
2007
ABSTRACT The epigenetic status of integrated adenovirus type 12 (Ad12) DNA in hamster cells cultivated for about 4 decades has been investigated. Cell line TR12, a fibroblastic revertant of the Ad12-transformed epitheloid hamster cell line T637 with 15 copies of integrated Ad12 DNA, carries one Ad12 DNA copy plus a 3.9-kbp fragment from a second copy. The cellular insertion site for the Ad12 integrate, identical in both cell lines, is a >5.2-kbp inverted DNA repeat. The Ad12 transgenome is packaged around nucleosomes. The cellular junction is more sensitive to micrococcal nuclease at Ad12-occupied sites than at unoccupied sites. Bisulfite sequencing reveals complete de novo methylation i…
Sec61alpha and TRAM are Sequentially Adjacent to a Nascent Viral Membrane Protein during its ER Integration
2007
Co-translational integration of a nascent viral membrane protein into the endoplasmic reticulum membrane takes place via the translocon. We have been studying the early stages of the integration of a double-spanning plant viral movement protein to gain insights into how viral membrane proteins are transferred from the hydrophilic interior of the translocon into the hydrophobic environment of the bilayer, where the transmembrane (TM) segments of the viral proteins can diffuse freely. Photocrosslinking experiments reveal that this integration involves the sequential passage of the TM segments past Sec61alpha and translocating chain-associating membrane protein (TRAM). Each TM segment is first…
Issues Related to the Restoration of Mirrors of the Wooden paliotto della chiesa del Santissimo Crocifisso all’Albergheria, Sicily (Italy)
2017
ABSTRACTIn this work, the decision-making process involved in the restoration of the eighteenth century paliotto ligneo (wooden altar frontal) della chiesa del Santissimo Crocifisso all’Albergheria of Palermo is presented. Earlier research concerning mirror restoration was based on only a few case studies and the proposed techniques were not suitable for the artwork here. As a consequence, it was necessary to re-examine theories and protocols of modern restoration to plan an appropriate intervention of the altar frontal. Since in this artwork the role of mirrors is not to give back images, as usual, but rather to create special light effects and play of lights, this work aims to find an app…
Multivariate EEG spectral analysis evidences the functional link between motor and visual cortex during integrative sensorimotor tasks
2012
The identification of the networks connecting brain areas and the understanding of their role in executing complex tasks is a crucial issue in cognitive neuroscience. In this study, specific visuomotor tasks were devised to reveal the functional network underlying the cooperation process between visual and motor regions. Electroencephalography (EEG) data were recorded from twelve healthy subjects during a combined visuomotor task, which integrated precise grip motor commands with sensory visual feedback (VM). This condition was compared with control tasks involving pure motor action (M), pure visual perception (V) and visuomotor performance without feedback (V + M). Multivariate parametric …
Competition and horizontal integration in maritime freight transport
2010
This paper develops a theoretical model for freight transport characterized by competition between means of transport (the road and maritime sectors), where modes are perceived as differentiated products. Competitive behavior is assumed in the road freight sector, and there are constant returns to scale. In contrast, the freight maritime sector is characterized by oligopolistic behavior, where shipping lines enjoy economies of scale. The market equilibrium where the shipping lines behave as profit maximizers, provides a first approximation to the determinants of market shares, profits, and user welfare. We then characterize the equilibrium when horizontal integration of shipping lines occur…
Constructing Knowledge through a Role-Play in a Web-Based Learning Environment
2003
This study aimed to find out how and on what level the students of two separate secondary schools shared and constructed knowledge on imperialism by interacting through historical role characters in a Web-based environment. Furthermore, the study aimed to find out how social and contextual features affected the nature of knowledge sharing and construction. The data about the history project were gathered by various means in order to validate the findings of the case study. The results demonstrated that the level of the Web-based messages remained quite low. Also the use of the Web-based environment in terms of shared knowledge construction was rather weak. In comparison, different instruct…
Ontology languages for the semantic web: A never completely updated review
2006
This paper gives a never completely account of approaches that have been used for the research community for representing knowledge. After underlining the importance of a layered approach and the use of standards, it starts with early efforts used for artificial intelligence researchers. Then recent approaches, aimed mainly at the semantic web, are described. Coding examples from the literature are presented in both sections. Finally, the semantic web ontology creation process, as we envision it, is introduced.
Semantic Web Enabled Web Services: State-of-Art and Industrial Challenges
2003
Semantic Web technology has a vision to define and link Web data in a way that it can be understood and used by machines for automation, integration and reuse of data across various applications. Ontological definition of every resource as it is assumed in Semantic Web, along with new techniques for semantics processing and new vision Intelligent Web Services is expected to bring Web on its new level. At present, Web Services technology is stressed by the search of a right way for further development. Combination of Semantic Web and Web Services concepts may address many of difficulties of existing technology. It is not a question of whether Semantic Web is coming or not, but a question of …
Party Positions on Differentiated European Integration in the Nordic Countries: Growing Together, Growing Apart?
2020
The Nordic countries constitute an interesting laboratory for the study of differentiated European Integration. Even though Denmark, Finland, Iceland, Norway, and Sweden share some historical, cultural, socio-economic and political characteristics, all those countries have ultimately opted for a different kind of relationship with the EU. Whereas Finland, a member of the Eurozone since its inception in 1999, has been considered to be part of the Union’s ‘inner core’ for quite some time, Iceland and Norway, in contrast, have opted to remain outside the EU albeit closely associated via the European Economic Area Agreement. The variation of relationships has also been reflected in Nordic parti…
The Euro Area Crisis; Need for a Supranational Fiscal Risk Sharing Mechanism?
2013
The aim of this paper is to assess the effectiveness of risk sharing mechanisms in the euro area and whether a supranational fiscal risk sharing mechanism could insure countries against very severe downturns. Using an unbalanced panel of 15 euro area countries over the period 1979â2010, the results of the paper show that: (i) the effectiveness of risk sharing mechanisms in the euro area is significantly lower than in existing federations (such as the U.S. and Germany) and (ii) it falls sharply in severe downturns just when it is needed most; (iii) a supranational fiscal stabilization mechanism, financed by a relatively small contribution, would be able to fully insure euro area countries …