Search results for "Internal Transcribed Spacer"

Immunodeficiency Syndromes

2010

A new annual species of Bellium (Asteraceae) from the Balearic Islands

2007

A new species, Bellium artrutxensis (Asteraceae: Astereae), is described from the therophytic pastures of southern Minorca (Balearic Islands). The analysis of nuclear ribosomal internal transcribed spacer (ITS) sequences showed that the new taxon was closely related to Bellium bellidioides , but differed with regard to the annual lifespan, the absence of epigeal stolons, and the presence of a patent eglandular indumentum on the leaves. The new taxon shows morphological features that are well suited for the colonization of dry environments with a marked summer drought, and may be of adaptive significance. The new species is diploid (2 n = 18) and shows up to two accessory chromosomes in root…

Multiple approaches to identify bacteria in archaeological waterlogged wood

2013

This study was carried out in collaboration with Soprintendenza del Mare (SM) that started, since 2004, to plan and realize underwater archaeological parks, such as in the Sicilian islands of Pantelleria (Gadir), Levanzo (Cala Minnola), Ustica (Falconiera), Panarea (Basiluzzo) and Filicudi (Capo Graziano). In situ conservation, as well as virtual exhibitions of the same topics, can contribute to ensure the protection and best fruition of underwater cultural heritage. The focus of this study was the identification of bacterial colonies in waterlogged wood samples from the rostrum of a excellent workmanship, that is very likely one of the wrecks attributed to Sextus Pompey fleet (36 BC) and d…

Culturable endophytic microbial communities in the circumpolar grass,Deschampsia flexuosain a sub-Arctic inland primary succession are habitat and gr…

2014

Summary Little is known about endophytic microbes in cold climate plants and how their communities are formed. We compared culturable putative endophytic bacteria and fungi in the ecologically important circumpolar grass, Deschampsia flexuosa growing in two successional stages of subarctic sand dune (68°29′N). Sequence analyses of partial 16S rRNA and internal transcribed spacer (ITS) sequences of culturable endophytes showed that diverse bacteria and fungi inhabit different tissues of D. flexuosa. A total of 178 bacterial isolates representing seven taxonomic divisions, Alpha, Beta and Gammaproteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria, and 30 fungal isolates …

What is the origin of the Scottish populations of the European endemic Cherleria sedoides (Caryophyllaceae)?

2015

Cherleria sedoides L. (Minuartia sedoides (L.) Hiern) is a montane perennial which, with some species in Minuartia sect. Spectabiles, is more closely related to Scleranthus than to other Minuartia species and is therefore best restored to the reinstated and redefined genus Cherleria. Reconstruction of the ancestral area of the clade containing C. sedoides suggests that it evolved in the Alps or the Balkan peninsula. The species now has an unusual distribution, being present in the mountains of southern Europe and Scotland but absent from the Arctic. Three historical scenarios that might have led to the presence of the species in Scotland are outlined and tested by a molecular analysis compa…

Characterization of a β-glucosidase isolated from an alpeorujo strain of Candida adriatica

2017

ABSTRACTA β-glucosidase-producing strain, Candida adriatica CECT13142, was isolated from olive oil wastes (alpeorujo) and identified by PCR/restriction fragment length polymorphism of the rDNA internal transcribed spacer and sequence analysis of the D1/D2 region of the 26S rRNA gene techniques. The enzyme was purified by sequential chromatography on DEAE-cellulose and Sephadex G-100. The relative molecular mass of the enzyme was estimated to be 50 kDa by SDS-PAGE. The hydrolytic activity of the β-glucosidase had an optimum pH of 8.2 and an optimum temperature of 40°C. The enzyme displayed high substrate specificity and high catalytic efficiency (Km 0.85 mM, Vmax 12.5 U/g of cells) for p-nit…

Diversity and complexity of microbial communities from a chlor-alkali tailings dump

2015

Abstract Revegetation of the tailings dumps produced by various industrial activities is necessary to prevent dust storms and erosion and represents a great challenge for ecological restoration. Little is known about the microbial colonisation and community structure of revegetated tailings following site exploitation. Here, we report the sequencing of 16S rRNA and internal transcribed spacer (ITS) fungal RNA gene amplicons from chlor-alkali residue and from an adjacent undisturbed soil to define the composition and assembly of the rhizosphere microbial communities. After quality filtering, a total of 72,373 and 89,929 bacterial sequences and 122,618 and 111,209 fungal sequences remained fo…

BythinellaMoquin-Tandon, 1856 (Gastropoda: Rissooidea: Bythinellidae) in Romania: species richness in a glacial refugium

2009

Mitochondrial cytochrome oxidase I (COI) and ribosomal internal transcribed spacer 1 (ITS-1) sequences were analysed in 12 Romanian Bythinella populations. Phylogenetic relationships were inferred using maximum parsimony, maximum likelihood and Bayesian techniques. For COI, the Kimura two-parameter (K2P) distances and haplotype networks were computed. Two sympatric and four allopatric groups were distinguished. The K2P distances are similar to those for congeneric rissooids, so each of the six groups represents a species. Two are identified as Bythinella molcsanyi H. Wagner, 1941, and Bythinella dacica Grossu, 1946. The other four groups cannot be ascribed to any known Bythinella. The occur…

Phylogenetics of Anthyllis (Leguminosae: Papilionoideae: Loteae): Partial incongruence between nuclear and plastid markers, a long branch problem and…

2010

Abstract Phylogenetic relationships in the genus Anthyllis (Leguminosae: Papilionoideae: Loteae) were investigated using data from the nuclear ribosomal internal transcribed spacer regions (ITS) and three plastid regions (psbA–trnH intergenic spacer, petB–petD region and rps16 intron). Bayesian and maximum parsimony (MP) analysis of a concatenated plastid dataset recovered well-resolved trees that are topologically similar, with many clades supported by unique indels. MP and Bayesian analyses of the ITS sequence data recovered trees that have several well-supported topological differences, both among analyses, and to trees inferred from the plastid data. The most substantial of these concer…

A nuclear ribosomal DNA pseudogene in triatomines opens a new research field of fundamental and applied implications in Chagas disease

2015

A pseudogene, designated as "ps(5.8S+ITS-2)", paralogous to the 5.8S gene and internal transcribed spacer (ITS)-2 of the nuclear ribosomal DNA (rDNA), has been recently found in many triatomine species distributed throughout North America, Central America and northern South America. Among characteristics used as criteria for pseudogene verification, secondary structures and free energy are highlighted, showing a lower fit between minimum free energy, partition function and centroid structures, although in given cases the fit only appeared to be slightly lower. The unique characteristics of "ps(5.8S+ITS-2)" as a processed or retrotransposed pseudogenic unit of the ghost type are reviewed, wi…